Jovane Hamelin Morrissette1, Dominic Tremblay2, Alexis Marcotte-Chénard1, Farah Lizotte2, Marie A Brunet2,3, Benoit Laurent1,4, Eléonor Riesco1,5, Pedro Geraldes6,7. 1. Research Center on Aging, CIUSSS de l'Estrie-CHUS, Sherbrooke, QC, J1H 4C4, Canada. 2. Faculty of Medicine and Health Sciences, Research Center of the Centre Hospitalier Universitaire de Sherbrooke (CHUS), 3001 12e Avenue N, Sherbrooke, QC, J1H 5H3, Canada. 3. Medical Genetics Service, Department of Pediatrics, Université de Sherbrooke, Sherbrooke, QC, Canada. 4. Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada. 5. Faculty of Physical Activity Sciences, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada. 6. Faculty of Medicine and Health Sciences, Research Center of the Centre Hospitalier Universitaire de Sherbrooke (CHUS), 3001 12e Avenue N, Sherbrooke, QC, J1H 5H3, Canada. pedro.geraldes@USherbrooke.ca. 7. Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada. pedro.geraldes@USherbrooke.ca.
Abstract
PURPOSE: Type 2 diabetes is associated with a higher risk of cardiovascular diseases, lowering the quality of life and increasing mortality rates of affected individuals. Circulating monocytes are tightly involved in the atherosclerosis process leading to cardiovascular diseases (CVD), and their inflammatory profile can be modified by exercise. The objective was to exploratory identify genes associated with CVD that could be regulated by high-intensity interval training (HIIT) in monocytes of type 2 diabetes patients. METHODS: Next-generation RNA sequencing (RNA-seq) analyses were conducted on isolated circulating monocytes (CD14+) of six women aged 60 and over with type 2 diabetes who completed a 12-week supervised HIIT intervention on a treadmill. RESULTS: Following the intervention, a reduction of resting diastolic blood pressure was observed. Concomitant with this result, 56 genes were found to be downregulated following HIIT intervention in isolated monocytes. A large proportion of the regulated genes was involved in cellular adhesion, migration and differentiation into an "atherosclerosis-specific" macrophage phenotype. CONCLUSION: The downregulation of transcripts in monocytes globally suggests a favorable cardiovascular effect of the HIIT in older women with type 2 diabetes. In the context of precision medicine and personalized exercise prescription, shedding light on the fundamental mechanisms underlying HIIT effects on the gene profile of immune cells is essential to develop efficient nonpharmacological strategies to prevent CVD in high-risk population.
PURPOSE: Type 2 diabetes is associated with a higher risk of cardiovascular diseases, lowering the quality of life and increasing mortality rates of affected individuals. Circulating monocytes are tightly involved in the atherosclerosis process leading to cardiovascular diseases (CVD), and their inflammatory profile can be modified by exercise. The objective was to exploratory identify genes associated with CVD that could be regulated by high-intensity interval training (HIIT) in monocytes of type 2 diabetes patients. METHODS: Next-generation RNA sequencing (RNA-seq) analyses were conducted on isolated circulating monocytes (CD14+) of six women aged 60 and over with type 2 diabetes who completed a 12-week supervised HIIT intervention on a treadmill. RESULTS: Following the intervention, a reduction of resting diastolic blood pressure was observed. Concomitant with this result, 56 genes were found to be downregulated following HIIT intervention in isolated monocytes. A large proportion of the regulated genes was involved in cellular adhesion, migration and differentiation into an "atherosclerosis-specific" macrophage phenotype. CONCLUSION: The downregulation of transcripts in monocytes globally suggests a favorable cardiovascular effect of the HIIT in older women with type 2 diabetes. In the context of precision medicine and personalized exercise prescription, shedding light on the fundamental mechanisms underlying HIIT effects on the gene profile of immune cells is essential to develop efficient nonpharmacological strategies to prevent CVD in high-risk population.
Authors: Hussein Al-Mossawi; Nicole Yager; Chelsea A Taylor; Evelyn Lau; Sara Danielli; Jelle de Wit; James Gilchrist; Isar Nassiri; Elise A Mahe; Wanseon Lee; Laila Rizvi; Seiko Makino; Jane Cheeseman; Matt Neville; Julian C Knight; Paul Bowness; Benjamin P Fairfax Journal: Nat Commun Date: 2019-10-08 Impact factor: 14.919
Authors: David B Bartlett; Leslie H Willis; Cris A Slentz; Andrew Hoselton; Leslie Kelly; Janet L Huebner; Virginia B Kraus; Jennifer Moss; Michael J Muehlbauer; Guillaume Spielmann; William E Kraus; Janet M Lord; Kim M Huffman Journal: Arthritis Res Ther Date: 2018-06-14 Impact factor: 5.156