Eleni Zografos1, Stavros C Proikakis2, Athanasios K Anagnostopoulos2, Anna-Maria Korakiti3, Flora Zagouri3, Maria Gazouli4, George T Tsangaris2. 1. Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece; el_zogra@hotmail.com. 2. Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece. 3. Department of Clinical Therapeutics, Alexandra Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece. 4. Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Abstract
BACKGROUND/AIM: Until now, little emphasis has been placed on the protein expression profile of male breast cancer (MBC) tumors, due to the rarity of the disease. The present study aimed to identify a proteomic pattern that is characteristic for malignant male breast tissue epithelium. MATERIALS AND METHODS: The protein content of four male breast tumors and corresponding adjacent healthy (control) tissues was analyzed by high-throughput nano-liquid chromatography-MS/MS technology. RESULTS: A total of 2,352 proteins were identified, that correspond to 1,249 single gene products, with diverse biological roles. Of those, a panel of 119 differentially expressed tissue proteins was identified in MBC samples compared to controls; 90 were found to be over-expressed in MBC tissues, while 29 were down-regulated. Concurrently, 844 proteins were detected only in MBC tumors and 197 were expressed exclusively in control mammary samples. CONCLUSION: Differential proteomic expression was found in MBC tissue, leading to improved understanding of MBC pathology and highlighting the need for personalized management of male patients.
BACKGROUND/AIM: Until now, little emphasis has been placed on the protein expression profile of male breast cancer (MBC) tumors, due to the rarity of the disease. The present study aimed to identify a proteomic pattern that is characteristic for malignant male breast tissue epithelium. MATERIALS AND METHODS: The protein content of four male breast tumors and corresponding adjacent healthy (control) tissues was analyzed by high-throughput nano-liquid chromatography-MS/MS technology. RESULTS: A total of 2,352 proteins were identified, that correspond to 1,249 single gene products, with diverse biological roles. Of those, a panel of 119 differentially expressed tissue proteins was identified in MBC samples compared to controls; 90 were found to be over-expressed in MBC tissues, while 29 were down-regulated. Concurrently, 844 proteins were detected only in MBC tumors and 197 were expressed exclusively in control mammary samples. CONCLUSION: Differential proteomic expression was found in MBC tissue, leading to improved understanding of MBC pathology and highlighting the need for personalized management of male patients.
Authors: Elizabeth Yeh; Melissa Cunningham; Hugh Arnold; Dawn Chasse; Teresa Monteith; Giovanni Ivaldi; William C Hahn; P Todd Stukenberg; Shirish Shenolikar; Takafumi Uchida; Christopher M Counter; Joseph R Nevins; Anthony R Means; Rosalie Sears Journal: Nat Cell Biol Date: 2004-03-14 Impact factor: 28.824
Authors: E Senkus; S Kyriakides; S Ohno; F Penault-Llorca; P Poortmans; E Rutgers; S Zackrisson; F Cardoso Journal: Ann Oncol Date: 2015-09 Impact factor: 32.976
Authors: Ida Johansson; Cecilia Nilsson; Pontus Berglund; Martin Lauss; Markus Ringnér; Håkan Olsson; Lena Luts; Edith Sim; Sten Thorstensson; Marie-Louise Fjällskog; Ingrid Hedenfalk Journal: Breast Cancer Res Date: 2012-02-14 Impact factor: 6.466
Authors: Yingjie Xu; Tarek A Bismar; Jie Su; Bin Xu; Glen Kristiansen; Zsuzsanna Varga; Lianghong Teng; Donald E Ingber; Akiko Mammoto; Rakesh Kumar; Moulay A Alaoui-Jamali Journal: J Exp Med Date: 2010-10-11 Impact factor: 14.307
Authors: Karla Grisel Calderón-González; Ma Luz Valero Rustarazo; Maria Luisa Labra-Barrios; César Isaac Bazán-Méndez; Alejandra Tavera-Tapia; Maria Esther Herrera-Aguirre; Manuel M Sánchez del Pino; José Luis Gallegos-Pérez; Humberto González-Márquez; Jose Manuel Hernández-Hernández; Gloria León-Ávila; Sergio Rodríguez-Cuevas; Fernando Guisa-Hohenstein; Juan Pedro Luna-Arias Journal: J Proteomics Date: 2015-04-24 Impact factor: 4.044