Assessing Bone Quality of the Spine in Children with Scoliosis Using the Ultrasound Reflection Frequency Amplitude Index Method: A Preliminary Study.

Osteopenia is considered a common phenomenon in patients who have scoliosis. Quantitative ultrasound has been used to assess skeletal status for decades, and recently ultrasound imaging using reflection signals from vertebrae were as well applied to measure spinal curvatures in children with scoliosis. The objectives of this study were to develop a new method that can robustly extract a parameter from ultrasound spinal data for estimating bone quality of scoliotic patients and to investigate the potential of the parameter in predicting curve progression. The frequency amplitude index (FAI) was calculated based on the spectrum of the original radiofrequency signals reflected from the tissue-vertebra interface. The correlation between FAI and reflection coefficient was validated using decalcified bovine bone samples in vitro, and the FAIs of scoliotic subjects were investigated in vivo with reference to body mass index, Cobb angles and curve progression status. The results revealed that the intra-rater measures were highly reliable between different trials (intra-class correlation coefficient = 0.997). The FAI value was strongly correlated with the reflection coefficient of bone tissue (R2 = 0.824), and the lower FAI indicated the higher risk of curve progression for the non-mild scoliosis cases. This preliminary study found that the FAI method can provide a feasible and robust approach to assessment of the bone quality of spine and may be a promising factor in monitoring curve progression of patients who have adolescent idiopathic scoliosis.