Surface Characteristics and In-Vitro Studies of TiO2 Coatings by Plasma Electrolytic Oxidation in Potassium-Phosphate Electrolyte.

Plasma electrolytic oxidation (PEO) was used to produce titanium oxide (TiO2) coatings on Ti surface in potassium - phosphate electrolyte. The morphology, wettability, phase, and chemical compositions were studied as a function of processing parameters. The bioactivity of the coating was assessed by the ability to form biomimetic apatite in-vitro using cell culture medium. In-vitro studies using human mesenchymal stem cells were also conducted to evaluate cells' proliferation and viability of the treated Ti. The results revealed that the produced TiO2 coatings comprised pore features with the pore size increasing with applied current density and treatment duration due to high energy discharge channels at higher potential. The PEO treated Ti exhibited superhydrophilic characteristics with a contact angle <1°. The findings indicated that the large actual surface area produced by the PEO treatment and the presence of negatively charge P O 4 3 - are the key factors for the superhydrophilic behavior. The in-vitro studies revealed that the PEO treated groups had higher amount of biomimetic apatite formation compared to the as-polished Ti. The PEO treatment significantly enhanced the cells' adhesion and growth after 24 and 72 hrs compared to the untreated Ti. A significant difference in the bioactivity was not observed between anatase and rutile.