Literature DB >> 35175604

An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast.

Jakub Sumbal1, Thorarinn Gudjonsson2,3, Gunnhildur Asta Traustadottir4, Zuzana Koledova5.   

Abstract

Epithelial-stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  3D culture; Breast; Epithelial-stromal interactions; Fibroblast; Mammary gland; Spheroid

Mesh：

Year:  2022        PMID： 35175604     DOI： 10.1007/978-1-0716-2193-6_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties.

Authors:  Thorarinn Gudjonsson; René Villadsen; Helga Lind Nielsen; Lone Rønnov-Jessen; Mina J Bissell; Ole William Petersen
Journal:  Genes Dev       Date:  2002-03-15       Impact factor: 11.361

Review 2.  Integrated morphodynamic signalling of the mammary gland.

Authors:  Nikolce Gjorevski; Celeste M Nelson
Journal:  Nat Rev Mol Cell Biol       Date:  2011-08-10       Impact factor: 94.444

3.  SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland.

Authors:  Emilia Peuhu; Riina Kaukonen; Martina Lerche; Markku Saari; Camilo Guzmán; Pia Rantakari; Nicola De Franceschi; Anni Wärri; Maria Georgiadou; Guillaume Jacquemet; Elina Mattila; Reetta Virtakoivu; Yuming Liu; Youmna Attieh; Kathleen A Silva; Timo Betz; John P Sundberg; Marko Salmi; Marie-Ange Deugnier; Kevin W Eliceiri; Johanna Ivaska
Journal:  EMBO J       Date:  2016-12-14       Impact factor: 11.598

4.  FGF signaling in mammary gland fibroblasts regulates multiple fibroblast functions and mammary epithelial morphogenesis.

Authors:  Jakub Sumbal; Zuzana Koledova
Journal:  Development       Date:  2019-12-02       Impact factor: 6.868

Review 5.  Cancer-associated fibroblasts as key regulators of the breast cancer tumor microenvironment.

Authors:  J M Houthuijzen; J Jonkers
Journal:  Cancer Metastasis Rev       Date:  2018-12       Impact factor: 9.264

Review 6.  Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture.

Authors:  Eirikur Briem; Saevar Ingthorsson; Gunnhildur Asta Traustadottir; Bylgja Hilmarsdottir; Thorarinn Gudjonsson
Journal:  J Mammary Gland Biol Neoplasia       Date:  2019-01-25       Impact factor: 2.673

7.  ECM1 secreted by HER2-overexpressing breast cancer cells promotes formation of a vascular niche accelerating cancer cell migration and invasion.

Authors:  Sophie Sarah Steinhaeuser; Erika Morera; Zuzana Budkova; Alexander Schepsky; Qiong Wang; Ottar Rolfsson; Angela Riedel; Aileen Krueger; Bylgja Hilmarsdottir; Gunhild Mari Maelandsmo; Bryndis Valdimarsdottir; Anna Karen Sigurdardottir; Bjarni Agnar Agnarsson; Jon Gunnlaugur Jonasson; Saevar Ingthorsson; Gunnhildur Asta Traustadottir; Thordur Oskarsson; Thorarinn Gudjonsson
Journal:  Lab Invest       Date:  2020-03-18       Impact factor: 5.662

8.  Stromal PDGFR-α Activation Enhances Matrix Stiffness, Impedes Mammary Ductal Development, and Accelerates Tumor Growth.

Authors:  Anisha M Hammer; Gina M Sizemore; Vasudha C Shukla; Alex Avendano; Steven T Sizemore; Jonathan J Chang; Raleigh D Kladney; Maria C Cuitiño; Katie A Thies; Quinn Verfurth; Arnab Chakravarti; Lisa D Yee; Gustavo Leone; Jonathan W Song; Samir N Ghadiali; Michael C Ostrowski
Journal:  Neoplasia       Date:  2017-05-11       Impact factor: 5.715

9.  YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation.

Authors:  Erika Morera; Sarah Sophie Steinhäuser; Zuzana Budkova; Saevar Ingthorsson; Jennifer Kricker; Aileen Krueger; Gunnhildur Asta Traustadottir; Thorarinn Gudjonsson
Journal:  In Vitro Cell Dev Biol Anim       Date:  2019-09-03       Impact factor: 2.416

10.  SPRY1 regulates mammary epithelial morphogenesis by modulating EGFR-dependent stromal paracrine signaling and ECM remodeling.

Authors:  Zuzana Koledova; Xiaohong Zhang; Charles Streuli; Robert B Clarke; Ophir D Klein; Zena Werb; Pengfei Lu
Journal:  Proc Natl Acad Sci U S A       Date:  2016-09-12       Impact factor: 11.205
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