| Literature DB >> 35175468 |
O V Dolgikh1, D G Dianova2, A V Krivtsov2, I N Alikina2.
Abstract
A comparative analysis of the parameters characterizing sperm apoptosis of young (27-42 years) and middle-aged (44-51 years) men was performed by flow cytometry. Irrespective of age, activity of caspase-3 and p53-mediated controlling the transmission of apoptogenic signal transmission in gametes remained stable with the formation of germ cells with delayed (p<0.05) cell death according to the Annexin V-FITC+PI- criterion (predominantly in middle-aged men). Inhibition of the transmission of a proapoptogenic stimulus mediated by membrane cell death receptors (FAS) was also observed in this group. Comparison of indicators of sperm apoptosis showed age-related features of cell death, in particular, inhibition of membrane reception triggering FAS-dependent apoptosis, which is associated with insufficient phosphatidylserine production in middle-aged men, excessive life cycle duration, and aging of spermatozoa.Entities:
Keywords: caspase; flow cytometry; p53; receptor-mediated apoptosis; spermatozoon
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Year: 2022 PMID： 35175468 DOI： 10.1007/s10517-022-05418-1
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804