| Literature DB >> 3517176 |
R Lahesmaa-Rantala, K Granfors, A Toivanen.
Abstract
An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.Entities:
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Year: 1986 PMID: 3517176 DOI: 10.1016/0022-1759(86)90357-1
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303