Literature DB >> 3517176

Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA).

R Lahesmaa-Rantala, K Granfors, A Toivanen.   

Abstract

An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.

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Year:  1986        PMID: 3517176     DOI: 10.1016/0022-1759(86)90357-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  5 in total

1.  Investigations on the mechanism of the hypocholesterolemic action of 1-ethoxysilatrane.

Authors:  P P Mehta; T Ramasarma; C K Kurup
Journal:  Mol Cell Biochem       Date:  1990-09-03       Impact factor: 3.396

2.  Effect of a three month course of ciprofloxacin on the outcome of reactive arthritis.

Authors:  T Yli-Kerttula; R Luukkainen; U Yli-Kerttula; T Möttönen; M Hakola; M Korpela; M Sanila; J Parviainen; J Uksila; R Vainionpää; A Toivanen
Journal:  Ann Rheum Dis       Date:  2000-07       Impact factor: 19.103

3.  Yersinia specific immune complexes in the synovial fluid of patients with yersinia triggered reactive arthritis.

Authors:  R Lahesmaa-Rantala; K Granfors; H Isomäki; A Toivanen
Journal:  Ann Rheum Dis       Date:  1987-07       Impact factor: 19.103

4.  Circulating yersinia specific immune complexes after acute yersiniosis: a follow up study of patients with and without reactive arthritis.

Authors:  R Lahesmaa-Rantala; K Granfors; R Kekomäki; A Toivanen
Journal:  Ann Rheum Dis       Date:  1987-02       Impact factor: 19.103

5.  Bacterial antigens in synovial biopsy specimens in yersinia triggered reactive arthritis.

Authors:  R Merilahti-Palo; K O Söderström; R Lahesmaa-Rantala; K Granfors; A Toivanen
Journal:  Ann Rheum Dis       Date:  1991-02       Impact factor: 19.103

  5 in total

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