Literature DB >> 35167099

Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice.

Rajib Mukherjee1, Joan Sanchez-Gurmaches2,3,4.   

Abstract

Techniques to trace and isolate brown adipocyte precursor and adipocytes during development and disease are essential to fully understand brown adipose tissue development and function. Here we report several protocols using the R26R-mTmG reporter mice in thermogenic tissues based on confocal microscopy and fluorescence based flow cytometry. These techniques may be useful to understand the influence of genetic or environmental alterations in brown adipocyte precursors and adipocyte biology.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Adipocyte precursor cells; Clearing; Confocal microscopy; Development; Flow cytometry; Immunofluorescence; Lineage tracing; mTmG

Mesh：

Year:  2022        PMID： 35167099     DOI： 10.1007/978-1-0716-2087-8_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  Factors influencing brown fat activation in FDG PET/CT: a retrospective analysis of 15,000+ cases.

Authors:  Jeffrey D Steinberg; Wouter Vogel; Erik Vegt
Journal:  Br J Radiol       Date:  2017-06-07       Impact factor: 3.039

2.  Adipocyte Dynamics and Reversible Metabolic Syndrome in Mice with an Inducible Adipocyte-Specific Deletion of the Insulin Receptor.

Authors:  Masaji Sakaguchi; Shiho Fujisaka; Weikang Cai; Jonathon N Winnay; Masahiro Konishi; Brian T O'Neill; Mengyao Li; Rubén García-Martín; Hirokazu Takahashi; Jiang Hu; Rohit N Kulkarni; C Ronald Kahn
Journal:  Cell Metab       Date:  2017-01-05       Impact factor: 27.287

3.  Quantitative Analysis of the Whole-Body Metabolic Fate of Branched-Chain Amino Acids.

Authors:  Michael D Neinast; Cholsoon Jang; Sheng Hui; Danielle S Murashige; Qingwei Chu; Raphael J Morscher; Xiaoxuan Li; Le Zhan; Eileen White; Tracy G Anthony; Joshua D Rabinowitz; Zoltan Arany
Journal:  Cell Metab       Date:  2018-11-15       Impact factor: 27.287

Review 4.  Unexpected evidence for active brown adipose tissue in adult humans.

Authors:  Jan Nedergaard; Tore Bengtsson; Barbara Cannon
Journal:  Am J Physiol Endocrinol Metab       Date:  2007-05-01       Impact factor: 4.310

5.  A global double-fluorescent Cre reporter mouse.

Authors:  Mandar Deepak Muzumdar; Bosiljka Tasic; Kazunari Miyamichi; Ling Li; Liqun Luo
Journal:  Genesis       Date:  2007-09       Impact factor: 2.487

6.  Brown adipose tissue is associated with cardiometabolic health.

Authors:  Tobias Becher; Srikanth Palanisamy; Daniel J Kramer; Mahmoud Eljalby; Sarah J Marx; Andreas G Wibmer; Scott D Butler; Caroline S Jiang; Roger Vaughan; Heiko Schöder; Allyn Mark; Paul Cohen
Journal:  Nat Med       Date:  2021-01-04       Impact factor: 53.440

7.  Quantitative Fluxomics of Circulating Metabolites.

Authors:  Sheng Hui; Alexis J Cowan; Xianfeng Zeng; Lifeng Yang; Tara TeSlaa; Xiaoxuan Li; Caroline Bartman; Zhaoyue Zhang; Cholsoon Jang; Lin Wang; Wenyun Lu; Jennifer Rojas; Joseph Baur; Joshua D Rabinowitz
Journal:  Cell Metab       Date:  2020-08-12       Impact factor: 31.373

8.  Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed.

Authors:  Joan Sanchez-Gurmaches; David A Guertin
Journal:  Nat Commun       Date:  2014-06-19       Impact factor: 14.919

Review 9.  Metabolic regulation and the anti-obesity perspectives of human brown fat.

Authors:  Camilla Scheele; Søren Nielsen
Journal:  Redox Biol       Date:  2017-04-11       Impact factor: 11.799

10.  Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.

Authors:  Rachel K Zwick; Michael C Rudolph; Brett A Shook; Brandon Holtrup; Eve Roth; Vivian Lei; Alexandra Van Keymeulen; Victoria Seewaldt; Stephanie Kwei; John Wysolmerski; Matthew S Rodeheffer; Valerie Horsley
Journal:  Nat Commun       Date:  2018-09-04       Impact factor: 14.919
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