Literature DB >> 3516141

Detection of a serum class I molecule in rat with anti-rat liver beta-2-microglobulin.

K Matsumoto, T Natori, T Agui, S Tsutimoto, A Matsuhashi, D L Gasser.   

Abstract

Rat serum was fractionated on a column of Sephacryl-300 and tested with a rabbit anti-rat beta-2-microglobulin (B2m) antiserum. This antiserum was directed against B2m purified from rat liver, and its specificity was confirmed by immunoprecipitation procedures. The antiserum recognized three peaks in the fractionated rat serum: a 200- to 300-kd (kilodalton) fraction, a 40- to 70-kd component, and the free 12-kd B2m. Indirect immunoprecipitation from the 200- to 300-kd fraction led to the identification of a 43-kd polypeptide associated with B2m. A xenoantiserum against RT1 class I antigen also precipitated a similar polypeptide from the same fraction, but this molecule differed in size and antigenic specificity from the one precipitated by anti-rat B2m.

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Year:  1986        PMID： 3516141     DOI： 10.1007/bf00502981

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  20 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Induction of immunological tolerance by porcine liver allografts.

Authors:  R Y Calne; R A Sells; J R Pena; D R Davis; P R Millard; B M Herbertson; R M Binns; D A Davies
Journal:  Nature       Date:  1969-08-02       Impact factor: 49.962

3.  Isolation and partial characterization of a beta2-microglobulin-containing, H-2 antigen-like murine serum protein.

Authors:  S Kvist; P A Peterson
Journal:  Biochemistry       Date:  1978-10-31       Impact factor: 3.162

4.  Biochemical analysis of a 40,000 mol. wt mouse serum protein which binds beta 2-microglobulin and has serological cross-reactivity with H-2 antigens.

Authors:  L Ramanathan; M J Rogers; E A Robinson; V J Hearing; N Tanigaki; E Appella
Journal:  Mol Immunol       Date:  1982-09       Impact factor: 4.407

5.  Beta 2-microglobulin monitoring after renal transplantation.

Authors:  R T Schweizer; R Moore; S A Bartus; L Bow; J Hayden
Journal:  Transplant Proc       Date:  1981-09       Impact factor: 1.066

6.  Radioimmunoassay determinations of murine beta 2-microglobulin and an H-2 antigen-like serum component.

Authors:  S Kvist; P A Peterson
Journal:  Eur J Immunol       Date:  1979-12       Impact factor: 5.532

7.  Tissue-specific expression of an unusual H-2 (class I)-related gene.

Authors:  D Cosman; M Kress; G Khoury; G Jay
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205

8.  A mouse plasma substance carrying beta2-microglobulin activity and lacking in H-2 alloantigenic activity.

Authors:  T Natori; N Tanigaki; D Pressman
Journal:  J Immunogenet       Date:  1976-04

9.  Detection of a secreted form of the murine H-2 class I antigen with an antibody against its predicted carboxyl terminus.

Authors:  W L Maloy; J E Coligan; Y Barra; G Jay
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

10.  Purification and characterization of mouse beta-2 microglobulin: allelic variants from two different strains.

Authors:  L Ramanathan; G C Dubois; E A Robinson; E Appella
Journal:  Mol Immunol       Date:  1982-03       Impact factor: 4.407
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