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Literature DB >> 351611

Reconstitution of an Escherichia coli repair endonuclease activity from the separated uvrA+ and uvrB+/uvrC+ gene products.

Abstract

An in vitro complementation assay has been used for partial purification of uvrA+, uvrB+, and uvrC+ gene products from Escherichia coli. The uvrB+ and uvrC+ products cochromatograph on DEAE-cellulose and are completely resolved from the uvrA+ product, which has been further purified by phosphocellulose chromatography of the nonadsorbed protein fraction from the DEAE-cellulose. Neither the uvrB+/uvrC+ nor the uvrA+ product shows appreciable endonuclease activity on UV-irradiated DNA when tested separately. However, these factors complement each other to yield and ATP-dependent endonuclease activity specific for UV-irradiated DNA. Gel filtration experiments with the partially purified proteins indicate that the functional uvrA+ gene product has a molecular weight of 100,000. The uvrB+ gene product has an apparent molecular weight of 70,000, but it is presently unclear if this is the size of the uvrB+ product alone or the size of a complex of the uvrB+ and uvrC+ gene products.

Entities: Chemical Species

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Year: 1978 PMID： 351611 PMCID： PMC392603 DOI： 10.1073/pnas.75.6.2569

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

31 in total

Review 1. Enzymatic repair of DNA.

Authors: L Grossman; A Braun; R Feldberg; I Mahler
Journal: Annu Rev Biochem Date: 1975 Impact factor: 23.643

2. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.

Authors: R P BOYCE; P HOWARD-FLANDERS
Journal: Proc Natl Acad Sci U S A Date: 1964-02 Impact factor: 11.205

3. The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid.

Authors: I R LEHMAN; G G ROUSSOS; E A PRATT
Journal: J Biol Chem Date: 1962-03 Impact factor: 5.157

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers.

Authors: S Riazuddin; L Grossman
Journal: J Biol Chem Date: 1977-09-25 Impact factor: 5.157

6. Incision of ultraviolet-irradiated DNA by extracts of E. coli requires three different gene products.

Authors: E Seeberg; J Nissen-Meyer; P Strike
Journal: Nature Date: 1976-10-07 Impact factor: 49.962

7. Enzymatic repair of DNA: SITES OF HYDROLYSIS BY THE Escherichia coli endonuclease specific for pyrimidine dimers (correndonuclease II).

Authors: A G Braun; M Radman; L Grossman
Journal: Biochemistry Date: 1976-09-07 Impact factor: 3.162

8. A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. I. Purification and general properties.

Authors: S Ljungquist; T Lindahl
Journal: J Biol Chem Date: 1974-03-10 Impact factor: 5.157

9. The structural gene for a T7 endonuclease essential for phage DNA synthesis.

Authors: M S Center; F W Studier; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1970-01 Impact factor: 11.205

10. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.

Authors: J F Scott; S Eisenberg; L L Bertsch; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

64 in total

1. Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites.

Authors: M Bjorâs; L Luna; B Johnsen; E Hoff; T Haug; T Rognes; E Seeberg
Journal: EMBO J Date: 1997-10-15 Impact factor: 11.598

7. Acetylaminofluorene bound to different guanines of the sequence -GGCGCC- is excised with different efficiencies by the UvrABC excision nuclease in a pattern not correlated to the potency of mutation induction.

Authors: E Seeberg; R P Fuchs
Journal: Proc Natl Acad Sci U S A Date: 1990-01 Impact factor: 11.205

Reconstitution of an Escherichia coli repair endonuclease activity from the separated uvrA+ and uvrB+/uvrC+ gene products.

Review 1. Enzymatic repair of DNA.

2. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.

3. The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid.

4. Protein measurement with the Folin phenol reagent.

5. Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers.

6. Incision of ultraviolet-irradiated DNA by extracts of E. coli requires three different gene products.

7. Enzymatic repair of DNA: SITES OF HYDROLYSIS BY THE Escherichia coli endonuclease specific for pyrimidine dimers (correndonuclease II).

8. A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. I. Purification and general properties.

9. The structural gene for a T7 endonuclease essential for phage DNA synthesis.

10. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.

1. Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites.

2. Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins.

3. Stimulation of the UvrABC enzyme-catalyzed repair reactions by the UvrD protein (DNA helicase II).

4. Generation of single-nucleotide repair patches following excision of uracil residues from DNA.

5. Pathways for repair of DNA damaged by alkylating agent in Escherichia coli.

6. Nucleotide sequence of the tag gene from Escherichia coli.

7. Acetylaminofluorene bound to different guanines of the sequence -GGCGCC- is excised with different efficiencies by the UvrABC excision nuclease in a pattern not correlated to the potency of mutation induction.

8. Purification and characterization of 3-methyladenine DNA glycosylase I from Escherichia coli.

9. Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA.

10. The genetics and specificity of the constitutive excision repair system of Bacillus subtilis.