Literature DB >> 3515617

Tissue factor activity in HeLa cells measured with a continuous chromogenic assay and ELISA reader.

S D Carson, P G Archer.   

Abstract

Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.

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Year:  1986        PMID： 3515617     DOI： 10.1016/0049-3848(86)90228-8

Source DB:  PubMed          Journal:  Thromb Res        ISSN： 0049-3848            Impact factor:   3.944


  6 in total

Review 1.  Tissue factor encryption and decryption: facts and controversies.

Authors:  L Vijaya Mohan Rao; Hema Kothari; Usha R Pendurthi
Journal:  Thromb Res       Date:  2012-03-06       Impact factor: 3.944

2.  p21-activated kinase-1 signaling regulates transcription of tissue factor and tissue factor pathway inhibitor.

Authors:  Beatriz Sánchez-Solana; Mona Motwani; Da-Qiang Li; Jeyanthy Eswaran; Rakesh Kumar
Journal:  J Biol Chem       Date:  2012-10-04       Impact factor: 5.157

3.  Heterogeneous regulation of constitutive thrombomodulin or inducible tissue-factor activities on the surface of human saphenous-vein endothelial cells in culture following stimulation by interleukin-1, tumour necrosis factor, thrombin or phorbol ester.

Authors:  G Archipoff; A Beretz; J M Freyssinet; C Klein-Soyer; C Brisson; J P Cazenave
Journal:  Biochem J       Date:  1991-02-01       Impact factor: 3.857

4.  Cellular localization and trafficking of tissue factor.

Authors:  Samir K Mandal; Usha R Pendurthi; L Vijaya Mohan Rao
Journal:  Blood       Date:  2006-02-21       Impact factor: 22.113

Review 5.  Tissue factor: mechanisms of decryption.

Authors:  L Vijaya Mohan Rao; Hema Kothari; Usha R Pendurthi
Journal:  Front Biosci (Elite Ed)       Date:  2012-01-01

6.  Functional tissue factor is entirely cell surface expressed on lipopolysaccharide-stimulated human blood monocytes and a constitutively tissue factor-producing neoplastic cell line.

Authors:  T A Drake; W Ruf; J H Morrissey; T S Edgington
Journal:  J Cell Biol       Date:  1989-07       Impact factor: 10.539
  6 in total

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