Maryam Hamzeloo-Moghadam1, Mostafa Rezaei Tavirani2, Mohhamadreza Razzaghi3, Sina Rezaei Tavirani4, Nahid Safari-Alighiarloo5, Babak Arjmand6, Mohammad Rostami Nejad7. 1. Traditional Medicine and Material Medical Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 5. Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran. 6. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. 7. Research Institute for Gastroenterology and Liver Diseases, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
Introduction: Investigating the molecular mechanism of cellular response to light radiation has attracted many researchers' attention. In the present study, the critically affected gene by 7.5 min blue light radiation in human keratinocytes was investigated via network analysis. Methods: Gene expression profiles of human keratinocytes exposed to 7.5 min blue light radiation plus controls were extracted from Gene Expression Omnibus (GEO). The significant dysregulated genes plus 100 first neighbors were investigated by Cytoscape software and its applications. The central nodes of the network based on four centrality parameters were determined and discussed. Results: Among 6 significant dysregulated genes, 4 individuals were recognized by the STRING database. The network was constructed by using the 4 queried genes and 100 first neighbors. EGR1, STAT1, and ISG15 were identified as central nodes; however, the prominent role of EGR1 was highlighted. Conclusion: EGR1 appeared as a critically affected gene after blue light irradiation. It seems that this upregulated gene is responsible for protecting human keratinocytes against stress and cancer. Therefore, the application of blue light may be accompanied by antistress effects in the human body.
Introduction: Investigating the molecular mechanism of cellular response to light radiation has attracted many researchers' attention. In the present study, the critically affected gene by 7.5 min blue light radiation in human keratinocytes was investigated via network analysis. Methods: Gene expression profiles of human keratinocytes exposed to 7.5 min blue light radiation plus controls were extracted from Gene Expression Omnibus (GEO). The significant dysregulated genes plus 100 first neighbors were investigated by Cytoscape software and its applications. The central nodes of the network based on four centrality parameters were determined and discussed. Results: Among 6 significant dysregulated genes, 4 individuals were recognized by the STRING database. The network was constructed by using the 4 queried genes and 100 first neighbors. EGR1, STAT1, and ISG15 were identified as central nodes; however, the prominent role of EGR1 was highlighted. Conclusion: EGR1 appeared as a critically affected gene after blue light irradiation. It seems that this upregulated gene is responsible for protecting human keratinocytes against stress and cancer. Therefore, the application of blue light may be accompanied by antistress effects in the human body.