Seyedeh Sareh Hendi1, Maryam Shiri2, Banafsheh Poormoradi3, Mohammad Yousef Alikhani4, Saeid Afshar5, Abbas Farmani6. 1. Department of Endodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran. 2. General Dentist, Hamadan University of Medical Sciences, Hamadan, Iran. 3. Dental Research Center, Department of Periodontology, Dentistry Faculty, Hamadan University of Medical Sciences, Hamadan, Iran. 4. Faculty of Medicine, Microbiology Department, Hamadan University of Medical Sciences, Hamadan, Iran. 5. Department of Molecular Medicine and Genetics, School of Advanced Technologies in Medicine, Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. 6. Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract
Introduction: The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis. Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 108 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). Results: There was a significant decrease in colony counts for all groups after interventions (P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group (P value <0.004). There was a significant reduction for group 1 in comparison with other groups (P value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts (P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.
Introduction: The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis. Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 108 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). Results: There was a significant decrease in colony counts for all groups after interventions (P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group (P value <0.004). There was a significant reduction for group 1 in comparison with other groups (P value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts (P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.
Authors: Aguinaldo S Garcez; Martha S Ribeiro; George P Tegos; Silvia C Núñez; Antonio O C Jorge; Michael R Hamblin Journal: Lasers Surg Med Date: 2007-01 Impact factor: 4.025
Authors: Michelle Bernardes Fonseca; Paulo Odair Tessare Júnior; Raul Capp Pallota; Hely Ferreira Filho; Odilon Victor Porto Denardin; Abrão Rapoport; Rogério Aparecido Dedivitis; João Francisco Veronezi; Walter João Genovese; Ana Lucia Franco Ricardo Journal: Photomed Laser Surg Date: 2008-06 Impact factor: 2.796
Authors: C T Rodrigues; F B de Andrade; L R S M de Vasconcelos; R Z Midena; T C Pereira; M C Kuga; M A H Duarte; N Bernardineli Journal: Int Endod J Date: 2018-02-28 Impact factor: 5.264