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Literature DB >> 35147951

Dissection, Fixation, and Immunostaining of the Drosophila Midgut.

Abstract

The Drosophila midgut is mainly composed of highly polarized epithelial cells called enterocytes that establish their apical-basal polarity in a fundamentally different way from other Drosophila epithelia. The roles of polarity factors in the midgut can be studied by generating clones of homozygous mutant cells in the background of wild-type tissue. In this chapter, we will introduce and discuss the procedures for producing positively marked mutant clones in the midgut and describe specific protocols for dissecting, fixing, and immunostaining this tissue.

Entities: Chemical

Keywords: Dissection; Drosophila midgut; Heat fixation; Immunostaining; MARCM

Mesh：

Substances：
Drosophila Proteins

Year: 2022 PMID： 35147951 DOI： 10.1007/978-1-0716-2035-9_20

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

24 in total

Review 1. Epithelial cell polarity and cell junctions in Drosophila.

Authors: U Tepass; G Tanentzapf; R Ward; R Fehon
Journal: Annu Rev Genet Date: 2001 Impact factor: 16.830

2. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.

Authors: O Baumann
Journal: Exp Cell Res Date: 2001-11-01 Impact factor: 3.905

Dissection, Fixation, and Immunostaining of the Drosophila Midgut.

Review 1. Epithelial cell polarity and cell junctions in Drosophila.

2. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.

3. Beta1-integrin orients epithelial polarity via Rac1 and laminin.

4. Evidence that stem cells reside in the adult Drosophila midgut epithelium.

Review 5. Epithelial ultrastructure and cellular mechanisms of acid and base transport in the Drosophila midgut.

6. Altered modes of stem cell division drive adaptive intestinal growth.

Review 7. The apical plasma membrane of chitin-synthesizing epithelia.

8. The adult Drosophila posterior midgut is maintained by pluripotent stem cells.

9. An alternative mode of epithelial polarity in the Drosophila midgut.

Review 10. Anatomy and Physiology of the Digestive Tract of Drosophila melanogaster.

1. De novo apical domain formation inside the Drosophila adult midgut epithelium.