Literature DB >> 35147947

Methods to Investigate Cell Polarity of Inner Ear.

Jihan Lyu1,2, Xiaoqing Qian1,2, Binjun Chen1,2, Dongdong Ren3,4.   

Abstract

Hair cells in cochlea and vestibular organs depend on the coordinated cell polarity to perform the normal auditory or balance function. The mouse inner ear is one of the ideal model to study planar cell polarity. In this chapter, we introduce a series of general experimental methods for studying planar cell polarity in the inner ear. The approaches presented here are also applicable to other organs with particular polarity phenotypes.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cochlea; Culture; Hair cell polarity; Inner ear dissection; Orientation of stereocilia bundles; Paint filling; SEM; Saccule; Utricle

Mesh：

Year:  2022        PMID： 35147947     DOI： 10.1007/978-1-0716-2035-9_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Establishment of hair bundle polarity and orientation in the developing vestibular system of the mouse.

Authors:  K Denman-Johnson; A Forge
Journal:  J Neurocytol       Date:  1999 Oct-Nov

2.  Alström Syndrome protein ALMS1 localizes to basal bodies of cochlear hair cells and regulates cilium-dependent planar cell polarity.

Authors:  Daniel Jagger; Gayle Collin; John Kelly; Emily Towers; Graham Nevill; Chantal Longo-Guess; Jennifer Benson; Karin Halsey; David Dolan; Jan Marshall; Jürgen Naggert; Andrew Forge
Journal:  Hum Mol Genet       Date:  2010-11-11       Impact factor: 6.150

3.  Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure.

Authors:  Patricia Ybot-Gonzalez; Dawn Savery; Dianne Gerrelli; Massimo Signore; Claire E Mitchell; Clare H Faux; Nicholas D E Greene; Andrew J Copp
Journal:  Development       Date:  2007-01-17       Impact factor: 6.868

4.  A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining.

Authors:  Paolo Cappella; Fabio Gasparri; Maurizio Pulici; Jürgen Moll
Journal:  Cytometry A       Date:  2008-07       Impact factor: 4.355

Review 5.  Primary cilia in planar cell polarity regulation of the inner ear.

Authors:  Chonnettia Jones; Ping Chen
Journal:  Curr Top Dev Biol       Date:  2008       Impact factor: 4.897

6.  Scanning electron microscopy: preparation and imaging for SEM.

Authors:  Chris G Jones
Journal:  Methods Mol Biol       Date:  2012

7.  Alms1-disrupted mice recapitulate human Alström syndrome.

Authors:  G B Collin; E Cyr; R Bronson; J D Marshall; E J Gifford; W Hicks; S A Murray; Q Y Zheng; R S Smith; P M Nishina; J K Naggert
Journal:  Hum Mol Genet       Date:  2005-07-06       Impact factor: 6.150

8.  Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates.

Authors:  Alison J Ross; Helen May-Simera; Erica R Eichers; Masatake Kai; Josephine Hill; Daniel J Jagger; Carmen C Leitch; J Paul Chapple; Peter M Munro; Shannon Fisher; Perciliz L Tan; Helen M Phillips; Michel R Leroux; Deborah J Henderson; Jennifer N Murdoch; Andrew J Copp; Marie-Madeleine Eliot; James R Lupski; David T Kemp; Hélène Dollfus; Masazumi Tada; Nicholas Katsanis; Andrew Forge; Philip L Beales
Journal:  Nat Genet       Date:  2005-09-18       Impact factor: 38.330

9.  Descriptive and experimental analysis of the epithelial remodellings that control semicircular canal formation in the developing mouse inner ear.

Authors:  P Martin; G J Swanson
Journal:  Dev Biol       Date:  1993-10       Impact factor: 3.582

10.  Patterning of the mammalian cochlea.

Authors:  R Cantos; L K Cole; D Acampora; A Simeone; D K Wu
Journal:  Proc Natl Acad Sci U S A       Date:  2000-10-24       Impact factor: 11.205
  10 in total

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