Correction to: Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues.

Correction to: BMC Genomics 22, 809 (2021)

https://doi.org/10.1186/s12864-021-08132-w

Following publication of the original article [1], it was reported that there were errors in the x- and y-axes of Figs. 4, 6 and 7.

Fig. 4

Sensitivity of different terminal modification TSO library construction methods. A The number of genes detected at 5 pg and 0.5 pg RNA inputs in different terminal modification TSO. B The ratio of the detected genes in the cell marker gene database of mice at 5 pg and 0.5 pg RNA inputs in different terminal modification TSO. C Precision for detecting genes in different terminal modification TSO. D Sensitivity for detecting genes in different terminal modification TSO. E, F The median number of genes detected per sample when downsampling total read counts to the indicated depths at 5 pg and 0.5 pg RNA inputs

Fig. 6

The effect of RNA with different template structures on gene detection. A The number of genes detected in the different structure of mRNA templates. B The ratio of the detected genes in the cell marker gene database of mice at the different structures of mRNA templates. C, D Number of genes detected in different expression levels binned by standardized expression FPKM at the different structures of mRNA templates. E, F Scatter plots show the correlation between different replicates and mRNA structure for 5 pg RNA inputs. G, H Scatter plots show the correlation between different replicates and mRNA structure for 0.5 pg RNA inputs

Fig. 7

Effect of RNA with different template structures on the accuracy and sensitivity of sequencing. A Precision for detecting genes at the different structures of mRNA templates. B Sensitivity for detecting genes at the different structures of mRNA templates. C, D The median number of genes detected per sample when downsampling total read counts to the indicated depths at 5 pg and 0.5 pg RNA inputs

In Fig. 4 the x axes of panels E and F were missing ‘2’ and the y axes contained a ‘$’ and “#” symbol after the values respectively.

In Fig. 6, in panel H the y axis contained a ‘$’ symbol after the reported values.

In Fig. 7, the x and y axis of panel C erroneously contained the ‘$’ symbol and in panel D both axes contained the “#” symbol.

The correct figures are presented in this Correction and the original article [1] has been corrected.