| Literature DB >> 35127991 |
Juliana Conkright-Fincham1, Chieri Tomomori-Sato1, Rich McGhee1, Ella M Leslie1, Carolyn J Beucher1, Lauren E Weems1, Shigeo Sato1, William B Redwine1, Kyle J Weaver1, Brandon D Miller1, Kym M Delventhal1, John J Kary2, Andrew B Koebbe3, Alexander Dean1,4, Jessica L Witt1, Laura M Remy1, Tari J Parmely1, Chongbei Zhao1, Yan Wang1, Joan W Conaway5, Jay R Unruh1.
Abstract
The SARS-CoV-2 pandemic and vaccination campaign has illustrated the need for high throughput serological assays to quantitatively measure antibody levels. Here, we present a protocol for a high-throughput colorimetric ELISA assay to detect IgG antibodies against the SARS-CoV-2 spike protein. The assay robustly distinguishes positive from negative samples, while controlling for potential non-specific binding from serum samples. To further eliminate background contributions, we demonstrate a computational pipeline for fitting ELISA titration curves, that produces an extremely sensitive antibody signal metric for quantitative comparisons across samples and time.Entities:
Keywords: COVID-19; ELISA; SARS-CoV-2; Serology; Spike protein
Year: 2022 PMID： 35127991 PMCID： PMC8799681 DOI： 10.21769/BioProtoc.4301
Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325