Literature DB >> 3512768

Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin ELISA more sensitive than the fluorescent antibody technique.

A Hornsleth, B Friis, P A Krasilnikof.   

Abstract

The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IF) technique and by a biotin/avidin (BA) ELISA in 156 samples of nasopharyngeal secretions (NPS) obtained from infants and small children with acute respiratory disease. Of 70 RSV-IF-positive NPS, 68 were positive by BA-ELISA. Of 86 RSV-IF-negative NPS, eight were positive by BA-ELISA. BA-ELISA could detect 0.5-1.0 ng RSV-protein.

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Year:  1986        PMID： 3512768     DOI： 10.1002/jmv.1890180203

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  12 in total

Review 1.  Pertussis: the disease and new diagnostic methods.

Authors:  R L Friedman
Journal:  Clin Microbiol Rev       Date:  1988-10       Impact factor: 26.132

2.  Evaluation of the Becton Dickinson Directigen test for respiratory syncytial virus in nasopharyngeal aspirates.

Authors:  T Kok; K Barancek; C J Burrell
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

3.  A rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion.

Authors:  A Hornsleth
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-05       Impact factor: 3.267

4.  Time-resolved fluoroimmunoassay compared with virus isolation for rapid detection of respiratory syncytial virus in nasopharyngeal aspirates.

Authors:  M Waris; P Halonen; T Ziegler; S Nikkari; G Obert
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

5.  Time-resolved fluoroimmunoassays with monoclonal antibodies for rapid identification of parainfluenza type 4 and mumps viruses.

Authors:  J C Hierholzer; P G Bingham; E Castells; R A Coombs
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

6.  Comparison of monoclonal antibody time-resolved fluoroimmunoassay with monoclonal antibody capture-biotinylated detector enzyme immunoassay for respiratory syncytial virus and parainfluenza virus antigen detection.

Authors:  J C Hierholzer; P G Bingham; R A Coombs; K H Johansson; L J Anderson; P E Halonen
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

7.  Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques.

Authors:  F Freymuth; M Quibriac; J Petitjean; M L Amiel; P Pothier; A Denis; J F Duhamel
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

8.  Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.

Authors:  J H Hughes; D R Mann; V V Hamparian
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948

9.  Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay.

Authors:  G Ahluwalia; J Embree; P McNicol; B Law; G W Hammond
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948

10.  Detection of respiratory syncytial virus and rotavirus by enhanced chemiluminescence enzyme-linked immunosorbent assay.

Authors:  A Hornsleth; K Aaen; M Gundestrup
Journal:  J Clin Microbiol       Date:  1988-04       Impact factor: 5.948
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