A Yektaseresht1, Z Hemati2, F Sabet Sarvestani1, A Hosseini1, S Vir Singh3. 1. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. 2. Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran. 3. Department of Biotechnology, Institute of Applied Sciences and Humanities, GLA University, Ajhai, Mathura, Uttar Pradesh, India.
Abstract
BACKGROUND: Mannheimia haemolytica primarily causes pneumonia leading to heavy morbidity and mortality in domestic livestock world-wide. Recently, outer membrane lipoproteins have emerged as targets for inducing protective immunity against the Pasteurella infection. AIMS: This study aimed to evaluate recombinant outer membrane lipoprotein E (PlpE) from the ovine M. haemolytica isolate, as a potential vaccine candidate. METHODS: Recombinant PlpE was constructed using pET26 (b) expression vector in Escherichia coli. Expressed recombinant PlpE was purified and injected subcutaneously to mice. The protection index of the vaccine was evaluated by challenge of mice intraperitoneally. RESULTS: Anti-PlpE antibody responses in the immunized mice was significantly increased in comparison with the control group which in turn, provided effective protection when challenged with strain of virulent M. haemolytica. CONCLUSION: Recombinant PlpE from ovine M. haemolytica isolate had the potential to be used as a vaccine candidate against M. haemolytica infection in sheep flocks.
BACKGROUND: Mannheimia haemolytica primarily causes pneumonia leading to heavy morbidity and mortality in domestic livestock world-wide. Recently, outer membrane lipoproteins have emerged as targets for inducing protective immunity against the Pasteurella infection. AIMS: This study aimed to evaluate recombinant outer membrane lipoprotein E (PlpE) from the ovine M. haemolytica isolate, as a potential vaccine candidate. METHODS: Recombinant PlpE was constructed using pET26 (b) expression vector in Escherichia coli. Expressed recombinant PlpE was purified and injected subcutaneously to mice. The protection index of the vaccine was evaluated by challenge of mice intraperitoneally. RESULTS: Anti-PlpE antibody responses in the immunized mice was significantly increased in comparison with the control group which in turn, provided effective protection when challenged with strain of virulent M. haemolytica. CONCLUSION: Recombinant PlpE from ovine M. haemolytica isolate had the potential to be used as a vaccine candidate against M. haemolytica infection in sheep flocks.
Authors: Patricia E Shewen; Chiang W Lee; Ann Perets; Douglas C Hodgins; Katherine Baldwin; Reggie Y C Lo Journal: Vaccine Date: 2003-05-16 Impact factor: 3.641
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