Characteristics of Essential Oils of Apiaceae Family: Their Chemical Compositions, in vitro Properties and Effects on Broiler Production.

There has been an upsurge of interest in the phytobiotics coincident with the onset of the potential ban on the use of antibiotic growth promoters (AGPs) in the broiler industry and because many kinds of nutraceuticals play an important role in improving growth performance, feed efficiency, and gut health of broilers. In the previous years, significant biological activities of essential oils (EOs) belonging to phytobiotics were observed, including anti-bacterial, antifungal, antiviral, and antioxidant properties. We found new perspectives on the roles of EOs, particularly extracts from the Apiaceae family, which is one of the largest plant families, in potential replacement of AGPs, and on the chemical composition involved in regulating microorganism activity and oxidative damage. Furthermore, the positive effects of EOs on broiler production and the possible mechanisms inducing the involvement of gut health and growth performance have been studied.

Contents

Introduction

1. Chemical compositions and in vitro properties of selected essential oils

1.1. Coriander essential oil (CEO)

1.2. Ajwain essential oil (AjEO)

1.3. Dill essential oil (DEO)

1.4. Fennel essential oil (FEO)

1.5. Anise essential oil (AnEO)

2. Effects of selected essential oils on broiler performance, carcass characteristics and serum traits

2.1. Broiler performance

2.2. Carcass characteristics

2.3. Serum traits

3. Effects of selected essential oils on intestinal microbiota and gut morphology of broilers

3.1. Intestinal microbiota

3.2. Gut morphology

Conclusions

Acknowledgements

Conflicts of interest

References

Introduction

Antibiotic growth promoters (AGPs) have been used in the broiler industry for decades to improve production performance and to minimize morbidity and mortality (Zeng ; Broom, 2018). However, the use of antibiotics in broiler production has raised problems in the human population due to bacterial resistance to the agents and transmission via the food chain (Graham ; Chowdhury ). Therefore, the use of AGPs in broilers has been prohibited in several countries. In 2006, the European Union imposed a complete ban on all AGPs. The USA is limiting AGP use and moving towards a significant reduction in general antibiotic usage (Salim ). Thereafter, many countries have announced AGP restrictions (Goutard ).

In broiler production, AGP supplementation improves body weight gain (BWG) and feed conversion ratio (FCR), indicating that the withdrawal of AGP may increase production costs (Cardinal ). This expectation has compelled nutritionists and feed manufacturers to seek the most suitable alternatives to AGPs. Since the early 2000s, researchers have explored the potential of nutraceuticals, such as probiotics, prebiotics, synbiotics, organic acids, and phytobiotics as alternatives to AGPs (Sugiharto, 2016), and the volatile extracts from plant sources have been identified as a new class of phytogenic feed additives (Zeng ).

The volatile extracts obtained from different plant parts, such as flowers, buds, seeds, leaves, twigs, bark, herbs, wood, fruits, and roots by hydro/steam distillation, are referred to as EOs. EOs have been reported to have antibacterial, antifungal, antiviral, and antioxidant properties as biological actions that depend on their chemical constituents (Al Bayati, 2008). Attention to EOs as a replacement for AGPs in poultry has increased because of their positive effects on production performance (Sugiharto, 2016). However, the mode of action of EOs is yet to be fully elucidated (Zeng ; Kikusato, 2021).

Apiaceae is one of the largest plant families (Pimenov and Leonov, 1993). Its plants have a characteristic pungent smell, whose extracts are EOs. Several constituents of EOs are believed to be the precursors of biological compounds that exert beneficial effects on gut morphology, nutrient absorption, microbiota, and oxidative status. Therefore, the EOs extracted from the Apiaceae family have been considered as a possible replacement for AGPs in broiler production (Acimovic ).

This review focuses on the characteristics of EOs, particularly the in vitro properties of EOs extracted from selected plants of the Apiaceae family, such as coriander (Coriandrum sativum), ajwain (Trachyspermum ammi), dill (Anethum graveolens), fennel (Foeniculum vulgare), and anise (Pimpinella anisum), and their effects on broiler production and possible machineries. Such an endeavor can never be truly comprehensive; however, this review aims to provide an awareness of the current state of the field for readers both inside and outside the phytobiotics community.

Chemical compositions and in vitro properties of selected essential oils

EOs are synthesized to protect the plant bodies against bacterial and fungal invasions and viruses and protect DNA and photosynthetic apparatus from the oxidative damage caused by ultraviolet radiation (Kikusato, 2021). Therefore, the EOs extracted from the plants of the Apiaceae family can perform various biological activities based on their chemical constituents. The relative concentration and overall yield of the constituents differ among plant types, parts, harvesting season, environmental conditions, soil type, storage conditions, and types of processing (Applegate ; Grashorn, 2010; Kiczorowska ; Al Yasiry and Kiczorowska, 2016). Most of the published literature describing in vitro antibacterial and antifungal properties has focused on the microbial species relevant to food pathogenesis; however, data regarding bacterial species that may influence the intestinal circumstances of broilers are lacking.

In this section, many measurement units are described as used in the literature: minimum inhibitory concentration (MIC) and/or zone of inhibition (ZOI) for antibacterial activity of the EOs. In addition, inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) assay, and Trolox equivalent antioxidant capacity (TEAC) using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), peroxide value (PV), thiobarbituric acid value (TBA), and antioxidant activity in the linoleic acid system are used for antioxidant activity.

Coriander essential oil (CEO: Table 1)

Chemical compositions

Coriander (Coriandrum sativum) is a glabrous, aromatic, and herbaceous annual plant with culinary applications and serves as a source of aroma compounds and EO. Coriander seeds contain 0.03 to 2.6% EO, with linalool being the main chemical constituent (Acimovic ; Jeya ). Table 1 shows the chemical composition, area of cultivation, extraction method, and yield of CEO from the selected studies. Linalool (Figure 1-1) was the major component of the CEO with a share of 66.3–75.3% of the total composition, whereas α-pinine, γ-terpinene, camphor, geranyl acetate, and cymene were the other major components (Baratta ; Delaquis ; Singh ; Kacaniova ). Singh reported the presence of more than 52 chemical compounds in CEO.

Table 1.

Chemical Compositions and in vitro properties of CEO (Coriander Essential Oil)

Chemical Composition	Baratta et al., 1998		Delaquis et al., 2002		Singh et al., 2006		Kacaniova et al., 2020
linalool	66.3		69.8		75.3		66.1
γ-terpinene	7.1		5.3		0.7		2.0
α-pinene	8.5		5.4		4.1
geranyl acetate	2.7				8.1		6.9
geraniol	2.0				0.8		2.6
camphor	3.8		5.2		0.1		8.3
limonene	1.9				0.6		3.0
camphene	0.9		1		0.1
myrcene	0.9		1.5		0.3		0.4
β-pinene	0.6				0.7
cymene	2.2				0.5		6.4
borneo1	0.6				0.3
terpinolene	0.4				0.2
α-terpineol	0.4				0.4		0.9
sabinene	0.3				0.2
terpinen-4-ol	0.3				0.2
β-phellandrene	0.2
trans-geraniol							2.6
1,2-oxolinalool							2.4
β-caryophyllene	0.1				0.4
2-myristynoyl pantetheine							0.4
citronellol							0.4
terpendiol							0.4
1,8-cineol					0.4
cis-linalool oxide					0.5
cuminal					0.6
α-thujene	0.1
α-terpinene	0.1
Cultivation/experimentation area	Italy		Canada		India		Slovakia
Extraction method/source	Commercial		Hydro distillation		Hydro distillation		Commercial
EO yield (%)	—		0.5		2.2		—
Antibacterial activity	Species	ZOI (mm) 10 µL/disk	Species	MIC (mL/dL)	NA		Species	ZOI (mm) 10 µL/disk
	B. subtilis	8.5					B. subtilis	10.7
	C. perfringens	4.0	L. monocytogenes	0.5			S. maltophilia	9.2
	E. coli	6.5	E. coli	0.2
	S. pullorurn	7.6	S. typhi	No inhibition observed
	Staph. aureus	16.1	Staph. aureus	0.4
Antifungal activity	Species	% Inhibition index 1 µL/mL	NA		Species	% ZOI+ 10 µL	NA
	A. niger	94.8			A. flavus	31.3 (75% by FPT1)
					A. terreus	75 (100% by FPT)
					A. niger	37.5 (100% by FPT)
Antioxidant activity	Method	Effects	NA		Method	Effects	Method	Effects
	Antioxidant index (AI%) using TBARS assay	higher than BHT at 1000 ppm			Peroxide value (PV) method	PV 248 meq/Kg of sunflower oil was reduced to 196 meq/Kg during storage at 80°C for 28days at 200 ppm dose of CEO	DPPH	CEO radical scavenging activity was 39.4 mg TEAC/L (Trolox equivalent antioxidant activity) equivalent to 51.1% of inhibition
					TBA value	TBA value 4 meq/kg of sunflower was reduced to 2.5 meq/Kg during storage at 80°C for 21days by 200 ppm dose of CEO

ZOI=zone of inhibition, MIC=minimum inhibitory concentration, FPT=food poison technique, S. maltophilia=stenotrophomonas maltophilia

Mycelial inhibition zone (%) at dose 10 µl by inverted petri plate method

Fig. 1.

Chemical structure of main compounds found in the selected essential oils.

In vitro properties

Many studies have shown that the chemical constituents present in CEO have antibacterial properties. Baratta analyzed the CEO (10 µL/disk) against 25 different bacteria, and the reported ZOI for Bacillus subtilis, Clostridium perferengens, Escherichia coli, Salmonella pullorum, and Staphylococcus (Staph.) aureus were 8.5, 4, 6.5, 7.6, and 16.1 mm, respectively. Kacaniova reported that the ZOI of CEO (10 µL/disk) against B. subtilis was 10.7 mm. Delaquis demonstrated that CEO had antibacterial activity against E. coli, Listeria monocytogenes, and Staph. aureus with MIC 0.2, 0.5, and 0.4 mL/dL (% vol/vol), respectively, except for C. perferengens. In a recent study, Jeya reported 0.64 mg/mL as the MIC of CEO against E. coli.

The CEO can effectively inhibit the growth of Aspergillus niger (inhibition index: 94.8%) at 1 µL/mL concentration (Baratta ). Singh evaluated the CEO (10 µL) against different fungi and reported good ZOI (more than 70%) against Curvularia palliscens, Fusarium moniliforme, and A. terreus. In addition, Jeya reported that the CEO showed fungicidal effects against Candida (Can.) albicans with a MIC of 0.02 mg/mL.

The CEO contains natural antioxidants that can prevent or delay the effects of oxidation processes. Baratta analyzed the antioxidant effectiveness of CEO through the modified thiobarbituric acid reactive species (TBARS) assay using two materials rich in lipids as oxidable substrates (egg yolk and rat liver). The results demonstrated that the CEO at 1000ppm in rat liver exhibited a higher antioxidant index than synthetic antioxidants, α-tocopherol, and butylated hydroxytoluene (BHT) at the same supplementation levels. Singh evaluated the antioxidant capacity of CEO by PV, TBA, and antioxidant activity in the linoleic acid system, revealing that 200 ppm CEO supplementation resulted in a 21% reduction in PV during storage at 80°C for 28 days. Kacaniova analyzed the radical scavenging activity of the CEO using the DPPH test and Trolox (vitamin E analog) as the standard, showing that 25 µL/mL CEO has 51.1% inhibition efficiency for scavenging free radicals. Moreover, Shahwar and Singh performed the radical scavenging activity of CEO at 500 µg/mL and 50 µL/mL using the DPPH test and reported 66.5% and 54.6% inhibition in DPPH-derived free radicals, respectively.

Ajwain essential oil (AjEO: Table 2)

Chemical compositions

Ajwain (Trachyspermum ammi) is an important plant with spice, aromatic, and medicinal properties. It originated in Egypt and is found worldwide. Ajwain seeds contain 2%–5% EO, with thymol (Fig. 1-2) as a major bioactive compound with a share of 39.1–67.4% of the total composition, followed by p-cymene, γ-terpinene, β-pinene, carvacrol, α-phellandrene, β-phellandrene, α-terpinene, α-pinene, and sabinene (Singh ; Vitali ; Gradinaru ). However, Patil reported that p-cymene (15.6%) was the major component in AjEO, followed by thymol (15.5%), by analyzing the peak area percentage of GC/MS results.

In vitro properties

The MIC of AjEO against Staph. aureus and E. coli were 500 µg/mL (Vitali ). However, Paul showed stronger antibacterial activity against gram-positive bacteria than against gramnegative bacteria. The MIC of AjEO against Streptococcus (Strep.) mutans, E. coli, S. typhi, S. parathyphi, P. vulgaris, and P. aeruginosa was 12.5 µL/mL (Patil ). Considering the composition of AjEO, thymol may be the main component to induce antibacterial activity. In a recent study, Gradinaru revealed that AjEO has the potential to limit the growth of respiratory pathogens (Staph. aureus, Strep. pneumoniae, P. aeruginosa) and discovered the combined effects of AjEO/thymol and conventional antibiotics against multidrug-resistant respiratory pathogens.

Singh showed that the AjEO at 6 µL dose rate is 100% fungicidal for all the tested pathogenic fungal species. In contrast, Vitali reported limited activity of AjEO against Can. albicans with a MIC of 500 µg/mL, which is 125 times higher than nystatin (reference anti-fungal drug).

According to Singh , AjEO has good antioxidant properties, as analyzed by the PV, TBA, and linoleic acid system. Patil demonstrated that AjEO is a strong antioxidant with 71.7% efficacy using the DPPH method, whereas the antioxidant activity of ascorbic acid (standard) was 20.2%. Vitali evaluated the antioxidant properties of AjEO using DPPH, ABTS, and FRAP assays. The ability of AjEO to scavenge the different radicals in all assays was compared with Trolox (vitamin E analog) and expressed as TEAC. The results revealed that the AjEO showed good antioxidant activity as the TEAC of ABTS, FRAP, and DPPH assays were 266.7 µmol TE/g, 90.6 µmol TE/g, and 72.6 µmol TE/g, respectively. The free radical scavenging activities of AjEO in all the studies mentioned above proved its potential as a natural antioxidant substance, which can be used as an efficient antioxidant agent.

Dill essential oil (DEO: Table 3)

Dill (Anethum graveolens) is one of the most useful spices with medicinal properties. It is cultivated worldwide, and its EO has flavoring and medicinal effects. Dill seeds yield 2%–4.2% EO with carvone (Fig. 1-3) as a major chemical component with a share of 47.7–73.6% in total composition, followed by limonene (Fig. 1-4), dill apiol, and α-phellendrene (Singh ; Yili ; Chahal ; Singh ). In contrast to previous studies, Kazemi (2015) reported thymol (20.1%) as the major component of DEO, followed by limonene, α-pinene, and carvacrol. He justified that his results are in contrast with those of other studies because of the genetic, environmental, chemotypes, and nutritional status of the plants. Since the chemical composition of DEO varies considerably between different studies, more comprehensive studies on chemical constituents are required.

Singh analyzed the antimicrobial activity of DEO against six pathogenic bacteria. They reported it as an effective antibacterial agent against P. aeruginosa and E. coli with ZOI 25.3 mm and 18.5 mm, respectively, although ineffective against S. typhi. DEO also showed effective antibacterial activity against Staph. aureus with MIC 0.27 mg/mL (Yili ). According to Kazemi (2015), DEO performed best against E. coli at a MIC of 5µg/mL. In contrast, the MIC for other tested bacteria (B. cereus, Enterococcus (En.) facealis, S. aureus, P. aerogenosa, and S. typhi) ranged between 10–40 µg/mL. In a recent study, DEO showed better inhibitory effects against gram-positive bacteria than gram-negative bacteria at 10 µL dose/disk (Singh ). ZOI for B. subtilis, Staph. aureus, E. coli, and P. aerogenosa were 15.6, 20.3, 7.5, and 8.9 mm, respectively.

DEO has the potential to produce antifungal effects. It has shown 100% fungicidal activity for Penicillium (Pen.) citrinum and A. niger at 6 µL concentration out of eight tested pathogenic fungi. The activity against other fungi was also considerable (Singh ). The Can. albican was also found to be very sensitive to DEO with a MIC value of 2.7 µg/mL (Yili ). Kazemi (2015) reported the significant fungicidal effects of DEO against Can. albicans and A. fumigatus at MIC 10 and 20µg/mL, respectively. Singh reported the significant antifungal activity of DEO against five tested pathogenic fungi. Among the tested fungi, A. flavus was the most sensitive (more than 80% ZOI) to DEO at 10 µL, followed by the other tested fungi. More recently, ten Candida species were examined against DEO and found very significant fungicidal effects with a MIC of 8.75 mg/mL for all tested fungi (Vieira ).

Singh evaluated the antioxidant properties of DEO by PV, TBA, and DPPH methods, revealing that 200 ppm DEO supplementation resulted in a 10.6% reduction in PV during storage at 80°C for 28 days. The TBA value of rapeseed oil was also reduced by approximately 50% during this storage period. Moreover, the radical scavenging activity of DEO by the DPPH method was 81.6% compared to butylated hydroxyanisole (BHA) (88.5%) and BHT (90.3%). Kazemi (2015) reported that the DPPH value of DEO (IC50=34.4 mg/mL) is comparable to that of Trolox (IC50=28.3 mg/mL), suggesting the antioxidant properties of this EO.

In a recent study, Singh evaluated the antioxidant activity of DEO by PV, TBA, and DPPH methods. They proved that it is a good natural antioxidant, similar to commercial antioxidant products. Briefly, 200 ppm DEO supplementation in mustard oil resulted in a 45% reduction in PV during storage at 60°C for 28 days, and the TBA value was reduced by approximately 50% and 25% on the 21st and 28th day of storage, respectively, compared to the control group. Moreover, DEO showed 75% radical scavenging activity, which was higher than that of the other tested commercial antioxidants. The conclusion of the studies mentioned above indicated the presence of carvone, limonene, and dill apiole in DEO, which may be the main reason for the antioxidant properties.

Fennel essential oil (FEO: Table 4)

Fennel (Foeniculum vulgare) is one of the oldest spice plants with considerable medicinal properties. The fennel contains 4%–6% EOs with more than 30 types of chemical constituents (Kooti ). Trans-anethole (Fig. 1-5) was identified as a major component with a share of 56.4–69.9% in total composition, whereas fenchone, estragole, and limonene were the other main components (Anwar ; Roby ; Diao ; Ilic ).

According to Anwar , FEO showed considerable antibacterial activity against B. subtilis and E. coli with ZOI of 29 mm and 14 mm, respectively. Roby demonstrated the antibacterial effects of FEO against gram-positive bacteria (B. cereus, Staph. aureus) and gram-negative (E. coli, S. typhi) bacteria with MICs ranging from 10 to 15 µg/mL. In another study, FEO showed antibacterial activity against E. coli, B. subtilis, and S. typhi at a MIC of 0.25 mg/mL; however, Staph. aureus and P. aurogenosa did not respond to it even at the highest tested concentration (10 mg/mL) (Diao ). More recently, Ilic reported that B. subtilis (MIC; 25 µg/mL) was the most sensitive bacteria to FEO, followed by Staph. aureus (50 µg/mL), E. coli (75 µg/mL), and Klebsiella pneumoniae (75 µg/mL). The authors concluded that the antibacterial activity of FEO depends on its chemical composition and the synergistic effects of the major chemical constituents.

Several studies have reported the significant antifungal properties of FEO, as shown by its activity against various fungal species such as Can. albicans, Aspergillus species, and dermatophytes (Kooti ). Anwar reported FEO as an efficient antifungal against the three tested fungi, particularly A. niger, showing the highest sensitivity with 28 mm ZOI and 80.6 mg/mL a MIC value. In another study, Can. albicans and A. flavus were sensitive to FEO at a MIC of 10 µg/mL (Roby ). More recently, Ilic reported that the Can. albicans was the most sensitive of the seven tested microorganisms in their study, with clear ZOI and 25 µg/mL MIC.

Limited data are available regarding the antioxidant properties of FEO; however, in some studies, it has been proven to be a strong antioxidant agent. Anwar evaluated the antioxidant properties of FEO using DPPH assay. They concluded that it has good radical scavenging activity with IC50=32.32 µg/mL. Moreover, FEO can replace commercial synthetic antioxidants such as BHA and BHT, which are discouraged because of their perceived carcinogenic potential and safety concerns (Anwar ).

Anise essential oil (AnEO: Table 5)

Anise (Pimpinella anisum) is an annual aromatic spice known for its medicinal and aromatic properties and is found worldwide. The fruit or seed of anise yields 2.1%–3.3% EO, and the important chemical components are trans-anethole (Fig. 1-5), methyl chavicol, and anisaldehyde (Arslan ; Sharifi ). Trans-anethole was identified as a major component with a share of 79–92.9% of the total composition, whereas estragole, 3, 4-dimethoxystyrene, α-gurjunene, and α-bisabolene were the other main components (Sharifi ; Topal ; Foroughi ; Asadollahpoor ). In contrast to the studies mentioned above, De Martino reported a slightly different chemical composition of AnEO and stated that the major chemical constituent of this EO is cis-anethole (97.1%).

Al Bayati (2008) reported the moderate antibacterial activity of AnEO against nine pathogenic bacteria with MIC ranging from 62.5–500 µg/mL, where gram-positive bacteria were more sensitive than gram-negative bacteria. In another study, a wide range of gram-positive and gram-negative bacterial species were found to be sensitive to AnEO, with MICs ranging from 25–100 mg/mL (Al Maofari, 2013). More recently, Foroughi confirmed the antibacterial effectiveness of AnEO against E. coli and Staph. aureus. The AnEO (31 mg/mL) performed better than the positive controls (kanamycin and cephalexin) in ZOI for E. coli and Staph. aureus. Moreover, the MICs for E. coli and Staph. aureus were 3 mg/mL and 7 mg/mL, respectively.

The antifungal activity of AnEO has been proven by many researchers. Elgayyar reported the antifungal potential of AnEO against A. niger with a significant zone of growth inhibition. Ozcan and Chalchat (2006) proved that AnEO is an effective antifungal agent against A. parasiticus, A. niger, and Alternaria alternate at 10–100 ppm doses. In another study, AnEO was reported as an antifungal agent against A. niger with a MIC of 2000 µL/L and EC50 of 400 µL/L (half-maximal effective concentration) (Sharifi ). In a study by Nanasombat and Wimuttigosol (2011), AnEO produced strong antifungal effects with clear ZOI against the six yeast and four mold species, and the MIC ranged from 1 mg/mL to 6 mg/mL for all the tested microbes.

Many studies have proved the antioxidant activity of AnEO and its ability to be used as a replacement for commercial antioxidants. According to Singh , 200 ppm AnEO supplementation in mustard oil resulted in a 28% reduction in PV during storage for 28 days at 70°C, which obtained a better result than commercial antioxidants. In addition, the DPPH assay proved the stronger antioxidant activity of AnEO than BHA and BHT. In a study by Topal , AnEO showed 77.5% free radical scavenging activity using DPPH assay, which was slightly lower than that of BHT (91%). In contrast, De Martino noted the least free radical scavenging activity of this EO (DPPH inhibition=19%) and speculated the reason is the low percentage of monoterpenes (1.2%) in its chemical constituents. They discussed that antioxidant activity is directly related to the monoterpene content of EOs. Nanasombat and Wimuttigosol (2011) also reported the antioxidant activity of AnEO measured by DPPH assay with IC50=86.88 mg/mL.

Thus, the chemical composition and in vitro properties of EOs are very unstable and depend on the genetic factors, environmental conditions, geographical location, harvest time, plant part used, and method of extraction. The EOs may consist of 20–60 chemical compounds with two or three major components present at high concentrations (70%–80%). The potential antibacterial, antifungal, and antioxidant activities of EOs rely entirely on their major bioactive chemical compounds, functional groups, and synergistic interactions between components (Chouhan ). Due to the variable nature of the chemical composition of EOs, it is difficult to determine the exact mechanism of action and dose rates for a specific activity (Kikusato, 2021).

Effects of selected essential oils on broiler performance, carcass characteristics and serum traits

Although several in vitro studies have shown the antimicrobial and antioxidant activities of EOs, the in vivo knowledge on the whole body of broiler health and growth performance is relatively less based on their chemical compositions and in vitro properties; however, possible mechanisms underlying the positive effects of EOs on biological actions can be generally hypothesized, including membrane disruption of pathogens, immunity-boosting activities, improvement of beneficial gut microbiota, appetite stimulation, and enhancement in the secretion of endogenous digestive enzymes (Williams, 2001; Cross ; Hong ; Sugiharto, 2016). Thus, some of this information is valuable to the application of EOs in the development of feed additives. It should also be noted that excess supplementation could decrease growth performance, possibly due to the potent nature of EOs, which negatively affects the digestive system by reducing FI and disturbing gut microflora at higher dose rates (Falaki ).

Broiler performance (Table 6)

For CEO supplementation, Ghazanfari reported a significant decrease in feed intake (FI), increase in body weight gain (BWG), and better feed conversion ratio (FCR) at 0.01, 0.02, and 0.03% of CEO in broiler diets compared to the negative control (NC: no supplementation of any EO or AGP). The highest output was noted with 0.03% supplementation, where a 9% increase in BWG and an 8% decrease in FCR were observed at the end of the experiment.

Falaki showed that the BWG of broilers increased by supplementing the AjEO up to 0.025% in the diet and started to decrease at 0.035% supplementation, although the FI was unchanged. One possible reason why growth performance was reduced by the overdose may be involved in thujone in AjEO, considering that this chemical component in sage oil is responsible for renal and liver dysfunction (Traesel ). In contrast, Chowdhury reported neither positive nor negative effects of 0.04% AjEO supplementation on growth performance compared to NC, although this supplementation level (0.04 %) was even higher than the level at which Falaki have negative effects on performance. The AjEO used in their study was not extracted by themselves; however, it was procured from a commercial company and was not chemically analyzed to check the purity and chemical composition. This suggests that the purity and chemical composition of EOs should be clarified to determine their effects on growth performance and other parameters in broilers.

Supplementation with FEO improved the BWG by up to 9% and reduced the FCR by up to 6% with 0.025% supplementation in broiler diets (Gharehsheikhlou ). In contrast, Stef reported the non-significant positive effects of FEO on the growth performance supplemented with 0.0125% and 0.025% in broiler diets. In both studies, the authors did not mention the purity and chemical composition of the FEO examined. The discrepancies in the results might be due to differences in the chemical composition and purity of the EOs. More detailed studies are required to clarify the reasons for these differences.

Several studies on AnEO supplementation have been conducted. In the 2000s, 0.04% AnEO-supplemented feed exhibited significantly improved body weight gain (Ciftci ; Simsek ). These observations were confirmed by Bhandari and Yadav (2013) and Eltazi (2014) using 0.04% AnEO. However, the effects of AnEO on feed intake are controversial: Bhadrai and Yadav (2013), Eltazi (2014), and Stef showed no changes, increases, or decreases in feed intake containing AnEO at 0.02-0.025% of diet, respectively.

Thus, many researchers have reported the positive effects of selected EOs obtained from the Apiaceae family on the growth performance of broilers. Therefore, detailed data from animal experiments should be carefully understood considering the dose rate and purity of each EO examined and their active compounds, including phenolics, terpenoids, glycosides, and alkaloids present as secondary plant metabolites.

Carcass characteristics (Table 7)

Although the CEO having linalool as a major chemical component has been proved a potent antimicrobial and growth promoter in broilers (Çabuk ; Ghazanfari ), there is a lack of published data regarding its effects on carcass characteristics of broilers according to the authors' knowledge. No positive effects of AjEO and FEO supplementation have been observed on the carcass characteristics of broilers, as well (Falaki ; Chowdhury ).

Simsek reported improved hot and cold carcass yields by supplementing broiler diets with 0.04% AnEO. This observation was confirmed by the results of Eltazi (2014) using the same level of supplementation. Moreover, the relative percentages of breast, thigh, and drumstick and the weight of the liver and gizzard were also improved by supplementing broiler diets with 0.04% AnEO (Simsek ; Eltazi, 2014). The highest FI was noted with 0.04% AnEO supplementation in the study by Eltazi (2014), which may be a possible reason for the improved liver and gizzard weight. The positive effects on carcass characteristics may be related to the effects of anethol, a major bioactive compound in AnEO, on the digestive system and liver metabolism of broilers.

Serum traits (Table 8)

Supplementation of CEO at 0.01% to 0.03% in broiler diets did not lead to significant changes in serum traits in broilers, including total cholesterol, triglycerides, glucose, high-density lipoproteins (HDL), low-density lipoproteins (LDL), and very low-density lipoproteins (VLDL) (Ghazanfari ). Chowdhury reported a reduced blood total cholesterol level of up to 19% in comparison to NC by the diet supplemented with 0.04% AjEO. The concentrations of triglycerides, glucose, and total proteins, however, remained unaffected in their study. The decrease in total cholesterol levels may be due to thymol, a major component of AjEO, which can act as an inhibitor of hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity, which is a key regulatory enzyme in cholesterol synthesis (Lee ).

Effects of selected essential oils on intestinal microbiota and gut morphology of broilers

The status of intestinal microbiota and gut morphology are important factors for evaluating gut health, including different aspects of the gastrointestinal tract (GIT), such as effective digestion of feed, absence of GIT ailment, normal and stable intestinal microbiota, and effective immune status (Bischoff, 2011). Intestinal microbiota play a crucial role in maintaining the health of broilers by altering several physiological functions, including digestion, metabolism, and immune responses (Carrasco ). Broilers are vulnerable to potentially harmful bacteria such as E. coli, Salmonella species, and C. perfringens, which compete with the host in GIT for nutrients, ultimately leading to poor growth performance and greater risk of disease incidence (Gunal ). The EO supplements can probably control intestinal microbiota, as these phytochemicals perform beneficial functions in the intestine, similar to prebiotics, even remaining less absorbed in the small intestine (Martel ). It should be noted that the absorption of phytobiotics, including EOs, is very low in the small intestine, as only 2%–15% of the compounds can be absorbed. This fact has been supported by recent studies revealing that phytochemicals may not need to be absorbed in the body to perform beneficial functions (Kikusato, 2021). Iqbal claimed that the intestinal microbiota would convert the phytochemicals into simpler metabolites to some extent to make them absorbable compounds, which may increase their bioavailability and improve the health-promoting effects in the intestine and inside the body. Furthermore, along with microbial community structure, EO supplementation could also be related to the microbial metabolites that improve the nutritional status of birds as well as GIT function and health (Ghazanfari ). Thus, the way of phytochemicals where to work on the host or microbiota, and what substrates are decomposed from it to absorb in the intestinal tract might be an important factor when the mechanism underlying their effect on either the host or microbiota is discussed.

Intestinal microbiota (Table 9)

Decreased numbers of pathogenic bacteria and an increased number of beneficial bacteria in the gut may improve the ability of epithelial cells to regenerate villi and thus enhance intestinal absorptive capacity (Mourao ). Considering the properties of phytobiotics, it is reasonable to expect such an effect by EOs due to their well-documented inhibitory effects against pathogenic microbiota. However, to the best of our knowledge, studies regarding the effects of selected EOs on the gut microbiota are limited.

CEO supplementation with 0.03% in broiler diets reduced the concentration of E. coli (log cfu/g) in caecum content by 4% in comparison to NC; however, the concentration of Lactobacillus (log cfu/g) remained unchanged (Ghazanfari ). Previously, it was observed that linalool, a major bioactive compound of CEO, inhibits the pathogenic microorganisms in the digestive system, which is possibly related to the reduction in the concentration of E. coli in the gut (Çabuk ; Lee ). Chowdhury reported no significant reduction in the concentration of E. coli, Clostridium, and Lactobacillus bacteria in precaecal digesta by supplementing broiler diets with 0.04% AjEO. They suggested that the low dose rate (0.04%) might be the reason for the unaffected concentration of E. coli and Clostridium bacteria; otherwise, thymol, a major bioactive compound of AjEO, is a potent antibacterial agent for these bacteria.

Gut morphology (Table 10)

The intestinal mucosal status and its microscopic structure may be a good indicator of the response of the GIT to active substances present in feed and the intestinal content (Viveros ). This mucosa is one of the main barriers in the intestine that prevents the invasion of pathogens and toxins in the GIT; therefore, these barriers can be destroyed by environmental, dietary, and oxidative stress, which results in systemic and intestinal inflammation (Kikusato, 2021). According to Huang and Lee (2018), phytobiotics, including EOs, have the potential to modulate inflammation-inducing factors in the intestine and can alleviate the inflammation cascade (For detail: Kikusato, 2021) and support gut health. Regarding changes in mucosal microscopic structure with EOs, the increased VH was reported to be related to enhanced digestive and absorptive functions of the intestine due to larger absorptive surface area and higher expression of brush border enzymes and nutrient transport systems (Pluske ).

Supplementation of CEO in broiler diets significantly affected VH, CD, and the VH/CD ratio in the duodenum, jejunum, and ileum parts of the intestine (Ghazanfari ). VH and CD increased significantly, whereas the VH/CD ratio decreased with CEO supplementation compared to NC. Çabuk demonstrated that linalool, a major component of CEO, can enhance VH in the intestine of broilers, and the activity of digestive enzymes, possibly improving digestibility and absorption of nutrients. Moreover, amylase concentration in the broiler intestine increases after dietary supplementation with CEO, which induces the villi to grow longer. According to Chowdhury , AjEO supplementation at 0.04% of the diet in broilers increased the VH and VH/CD ratio in the ileum by up to 27% and 24%, respectively. However, the morphology of the duodenum and jejunum remained unaffected.

How do EOs, such as CEO or AjEO, work on the mucosal structure? Windisch suggested that EOs increase VH due to their antioxidant properties. EOs can exhibit antioxidant effects through several mechanisms. These compounds contribute to the elimination of the reactive oxygen species (ROS) produced due to oxidative stress, not only by direct antioxidant action, but also by inducing the expression of antioxidant enzymes, such as catalase and superoxide dismutase (Windisch ; Kikusato, 2021). These antioxidant enzymes neutralize the ROS released during digestive processes, which can cause damage to the intestinal mucosa and ultimately shorten the villi. The EOs may protect the villi from oxidative damage by stimulating the activity of the antioxidant enzymes, and the phenolic group of the EOs may act as hydrogen donors showing antioxidant activity (Windisch ). The involvement of antioxidants was confirmed by Valenzuela-Grijalva , who speculated that the supplemented EOs can enhance the production performance not only by better FI, possibly due to improved flavor and palatability of diet, better intestinal functions, and activation of the endocrine system, but also by anti-oxidative defense mechanisms.

Based on the discussed literature, it is clear that all the EOs are not equally effective in the antimicrobial, antioxidant, and growth-promoting effects inside the body of broilers. The benefits of EOs in terms of growth performance may depend on their biological activities. Moreover, it is difficult to determine the precise and invariant effects of each EO, as they constitute variable percentages of mixtures in EOs for each plant. In addition to effectiveness, EOs are safe to be used as growth promoters for broilers and for the user (feed manufacturers/farm managers) and the consumers of the meat products compared to AGPs. In any case, as long as antimicrobial resistance will never emerge in response to their usage, EOs can be supplemented to the broiler diet throughout the rearing period without following the withdrawal period to guarantee food safety.

Conclusions

The potential ban on the use of AGPs in the broiler industry has highlighted the development of alternatives to supplement in broiler diets to support gut health and growth performance. We have endeavored to demonstrate several key themes.

1. The published data suggest that the chemical composition and yield of EOs from selected members of the Apiaceae family are quite variable depending on the geographical origin, environmental conditions, sowing/harvesting time of the plants, and the extraction method.

2. The in vitro antibacterial, antifungal, and antioxidant properties vary between the same EO of different origins. The relative percentage of bioactive compounds in EOs determines the extent and type of biological activity.

3. The results of the literature regarding supplementation of selected EOs in broiler diets are arbitrary and suggest ambiguous results regarding growth performance and feed efficiency.

4. The EOs extracted from the plant parts of the Apiaceae family have the potential to be utilized as a replacement for AGPs in broiler production.

5. Although these EOs have proven beneficial effects in broilers, the literature is so limited that further investigations regarding dose rate, combination of different EOs, and possible mechanisms of action are required.

Table 2.

Chemical Compositions and in vitro properties of AjEO (Ajwain Essential Oil)

Chemical Composition	Singh et al., 2004		Patil et al., 2016			Vitali et al., 2016			Gradinaru et al., 2018
thymol	39.1		15.5			67.4			50.8
γ-terpinene	23.2		9.3			11.3			26.0
π-cymene	30.8		15.6			17.9			18.3
α-phellandrene			8.7
α-pinene	0.2		4.7			0.1			0.2
carvacol	0.3		10.7			0.9
sabinene			4.2
β-phellandrene	0.6		7.6			0.3
β-pinene	1.7		10.6			0.7			2.3
α-terpinene	0.2		6.7			0.3			0.3
α-thujene	0.2					0.2			0.4
α-pinene	0.2
myrcene	0.4					0.2			0.5
terpinolene	0.2
trans-sabinene hydrate	0.1					0.1
linalool	0.1
terpenen-4-ol	0.8					0.2			0.1
α-terpineol	0.1					0.1
β-selinene	0.1
Cultivation/experimentation area	India		India			Iran			India (Romania)
Extraction method/source	Hydrodistillation		Hydrodistillation			Hydrodistillation			Hydrodistillation
EO yield (%)	2.2		5.1			2.7			7.4
Antibacterial activity	NA		Species	ZOI (mm) 20 µL/disk	MIC (µL/mL)	Species	ZOI (mm) 10 µL/disk	MIC (µg/mL)	Species	MIC (mg/mL)
			S. paratyphi A	52	12.5	Staph. aureus	34.7	500	Staph. aureus	4
			S. typhi	54	12.5	E.coli	29.3	500
			E. coli	66	12.5
Antifungal activity	Species	ZOI (%) 6 µL/disk	NA			Species	ZOI (mm) 10 µL/disk	MIC (µg/mL)	NA
	A. flavus	100				Can. albicans	54.3	500
	A. niger	100
Antioxidant activity	Method	Effects	Method	Effects		Method	Effects		NA
	PV method	PV 248 meq/Kg of linseed oil was reduced to 150 meq/Kg at 80°C during storage of 28days by 200 ppm addition of EO	DPPH	Strongest antioxidant activity (71.68%) noted at 1000 mg/L concentration and was three times greater than the effect produced by standard; ascorbic acid (20.24%)		ABTS	AjEO showed strong antioxidant activity with 1C50=22.4 µg/mL and TEAC=266.7 µmol TE/g
	TBA method	TBA value 3.8 and 5 meq/kg of linseed oil was reduced to 3.0 and 3.8 meq/Kg at 80°C during storage for 21 and 28 days, respectively, by 200 ppm addition of EO				FRAP	AjEO showed antioxidant activity with TEAC=90.6 µmol TE/g
						DPPH	AjEO showed weak antioxidant activity with aIC50=239.3 µg/mL and bTEAC=72.6 µmol TE/g

ZOI=zone of inhibition, MIC=minimum inhibitory concentration, TEAC=Trolox equivalent antioxidant concentration

Concentration of compound that affords a 50% reduction in the assay

Table 3.

Chemical Compositions and in vitro properties of DEO (Dill Essential Oil)

Chemical Composition	Singh et al., 2005		Yili et al., 2009		Kazemi, 2015		Singh et al., 2017
carvone	55.2		73.6				47.7
limonene	16.6		14.7		16.3		12.4
thymol					20.1
carvacrol					8.3
dill ether	0.2				3.1
dill apiole	14.4						32.7
α-pinene	0.1				8.7
linalool	3.7
trans-dihydrocarvone	2.8						2.7
cis-dihydrocarvone	2.6		5.9				2.1
α-phellandrene			0.03		2.4		1.3
sabinene	0.1				1.0
β-pinene	0.1
myrcene	0.1				0.7
γ-terpenene	0.3
terpinen-4-ol	0.1
iso-dihydrocarveol	0.1
cis-dihydrocraveol			0.2
trans-dihydrocarveol	0.1
geranyl acetate	0.3
β-caryophylene	0.6
β-bisabolene	0.3
δ-cadinene	0.1
trans-isocroweacin	0.8
1,2-diethoxyethane			1.4
dihydrocarvone			1.4
diplaniol			2.2
α-thujene					0.1
neophtadiene					1.4
n-nonadecane					1.0
n-eicosane					0.9
n-heneicosane					0.7
n-docosane					1.0
n-tricosane					1.0
n-tetracosane					1.5
π-cymenene							0.2
menthol							0.7
myristicin							0.9
Cultivation/experimentation area	India		Uzbekistan		Iran		India
Extraction method/source	Hydrodistillation		Hydrodistillation		Hydrodistillation		Hydrodistillation
EO yield (%)	2.6		4.2		3.2		2.4
Antibacterial activity	Species	ZOI (mm) 6 µL/disk	Species	MIC (mg/mL)	Species	MIC (µg/mL)	Species	ZOI (mm) 10 µL/disk
	B. subtilis	16.2					B. subtilis	15.6
	Staph. aureus	13.2	Staph. aureus	0.27	Staph. aureus	20	Staph. aureus	20.3
	S. typhi	No ZOI			S. typhi	40
	E. coli	18.5			E. coli	5	E. coli	7.5
	P. aeruginosa	25.3					P. aeruginosa	8.9
Antifungal activity	Species	% ZOI 6 µL/disk	Species	MIC (µg/mL)	Species	MIC (µg/mL)	Species	% ZOI (FPT1) 10 µL
	A. niger	100	Can. albican	2.7	A. fumigates	20	A.niger	63.9
	A. flavus	82.5			Can. albicans	10	A. flavus	89.7
	Pen. citrinum	100					Pen. viridicatum	17.6
Antioxidant activity	Method	Effects	Method	Effects	Method	Effects	Method	Effects
	PV	PV 239.2 meq/Kg of rapeseed oil was reduced to 213.9 meq/Kg during storage at 80°C for 28days by 200 ppm addition of EO	NA		FRAP	DEO=Antioxidant activity301 µmol Fe2+/g EO, Trolox (standard)= 321 µmol Fe2+/g EO	PV	PV 181.8 meq/Kg of mustard oil was reduced to 100 meq/Kg during storage at 60°C for 28days by 200 ppm addition of EO
	TBA value	TBA value 6.9 meq/kg of rapeseed oil was reduced to 3.4 meq/Kg during storage at 80°C for 28 days by 200 ppm addition of EO			DPPH	DEO scavenging activity 1C50=34.41 mg/mL, Trolox (standard) IC50=28.32 mg/mL	TBA value	TBA value 0.18 and 0.21 meq/kg of mustard oil was reduced to 0.092 and 0.16 meq/Kg during storage at 60°C for 21 and 28 days, respectively, by 200 ppm addition of EO
	DPPH	DEO showed 81.6% radical scavenging activity in comparison to BHA (88.5%) and BHT (90.3%)					DPPH	DEO showed 75% radical scavenging activity

ZOI=zone of inhibition, MIC=minimum inhibitory concentration, FPT=food poison technique

Concentration of compound that affords a 50% reduction in the assay

Table 4.

Chemical Compositions and in vitro properties of FEO (Fennel Essential Oil)

Chemical Composition	Anwar et al., 2009			Roby et al., 2013			Diao et al., 2014			Ilic et al., 2019
trans-anethole	69.9			56.4			68.5			64.9
estragole	5.5			5.2			10.4			2.6
limonene	5.1			4.2			6.2			2.3
fenchone	10.2			8.3			5.5			23.1
δ-3-carene							1.2
o-cymene							0.6
α-pinene	0.6			1.6			0.4			2.0
methyl chavicol				5.2
β-farnesene				3.0
γ-terpinene	0.2			1.4						0.7
camphene	0.1									0.2
sabinene	0.2									0.1
β-pinene	0.1						0.4
β-myrcene	0.9			0.6			0.2			1.0
α-phellandrene	0.2						0.1			0.4
β-ocimene	0.6
1,8-cineol	0.2			0.9
fenchyl alcohol	0.4
fenchyl acetate	0.5						0.1
cis-anethol	0.3						0.5			0.1
π-anisaldehyde	0.2						0.3			0.1
β-caryophyllene	0.3
germacrene	0.1						0.2
α-terpinin				0.6
terpin-4-ol				2.8
myrcenol				1.0
bergamoil				0.6
2,5-diethyl phenol				0.8
β-farnesene				3.0
α-farnesene				1.3
camphor							0.2			0.5
Cultivation/experimentation area	Pakistan			Egypt			China			Serbia
Extraction method/source	Hydrodistillation			Hydrodistillation			Hydrodistillation			Hydrodistillation
EO yield (%)	2.8			2.0			1.7			4.0
Antibacterial activity	Species	ZOI (mm) 15 µL/disk	MIC (mg/mL)	Species	ZOI (mm) 20 µg/disk	MIC (µg/mL)	Species	ZOI (mm) 100 µL in DMSO	MIC (mg/mL)	Species	ZOI (mm) 60 µL/disk	MIC (µg/mL)
	B. subtilis	29	62.6				B. subtilis	15.8	0.25	B. subtilis	32	25
	E. coli	14	259.3	E. coli	17	12.5	E. coli	19.1	0.25	Staph. aureus	23	50
				S. tyhpi	18	15	S. typhi	20.2	0.25	E. coli	20	75
				Staph. aureus	19	10	Staph. aureus	11.5	>10	k. pneumoniae	21	75
							P. aeruginosa	12.3	>10
Antifungal activity	Species	ZOI (mm) 15 µL/disk	MIC (mg/mL)	Species	ZOI (mm) 20 µg/disk	MIC (µg/mL)	NA			Species	ZOI (mm) dose rate: 60 µL/disk	MIC (µg/mL)
	A. niger	28	80.6	Can. albicans	22	10				Can. albicans	100%	25
				A. flavus	20	10
Antioxidant activity	Method	Effects		NA			NA			NA
	DPPH	FEO scavenging activity 1IC50=32.32 µg/mL, BHT (standard) IC50=19.00 µg/mL

ZOI=zone of inhibition, MIC=minimum inhibitory concentration

Concentration of compound that affords a 50% reduction in the assay

Table 5.

Chemical Compositions and in vitro properties of AnEO (Anise Essential Oil)

Chemical Composition	Sharifi et al., 2008		Topal et al., 2008		De Martino et al., 2009		Foroughi et al., 2016
trans-anethole	92.9		79.0				89.7
cis-anethole	0.1				97.1		0.4
estragole	2.2		3.6
α-pinene					0.3		0.1
anisaldehyde	0.1		0.7				0.4
α-himachalene			0.5
carvone	0.8
α-bisabolene	1.8
zingiberene	0.4
methyl-chavicol							2.2
3,4-dimethoxystyrene			5.2
α-gurjunene			4.0
limonene	0.8						0.8
fenchone					0.2		4.6
linalool			0.3		0.4
π-allyanisole	2.2
cis-dihydrocarvone	0.1
δ-element	0.1
aromadendrene	0.1
ar-curcumene	0.2
β-bisabolene	0.2
β-sesaquiphellandrene	0.1
α-terpinene			0.2
ylangene			0.2
elemene			0.2
β-caryophyllene			0.2
α-cis-himachalene			0.5
α-ethyl-π-anisyl alcohol			0.3
1-methylguanine			0.1
spathulenol			0.2
3-hydroxycarbofuran			0.8
ethyl oleate			0.9
methyl 1-phenylallyl ether			1.7
α-phellandrene					0.1		0.01
Δ3-carene					0.1
o-cymene					0.1
π-cymene							0.1
1,8-cineole							0.1
camphor							0.2
β-monopalmitate			0.2
di-α-furylmethane			0.2
Cultivation/experimentation area	Iran		Turkey		Italy		Iran
Extraction method/source	Hydrodistillation		Hydrodistillation		Commercial		Hydrodistillation
EO yield (%)	3.3		Not given		—		Not given
Antibacterial activity	NA		NA		Species	ZOI (mm) 490 µg/disk	Species	ZOI (mm) 31 mg/mL	MIC (mg/mL)
					B. cereus	6
					E. coli	0	E. coli	22	3
					Staph. aureus	0	Staph. aureus	22	7
Antifungal activity	Species	MIC (µL/L)	NA		NA		NA
	A. niger	2000 (1EC50=400 µL/L)
Antioxidant activity	NA		Method	Effects	Method	Effects	NA
			DPPH	AnEO showed 77.5% free radical scavenging activity, BHT (Standard) showed 91.4%	DPPH	AnEO showed 19% free radical scavenging activity

ZOI=one of inhibition; MIC=minimum inhibitory concentration

Half maximal effective concentration

Table 6.

Effects of selected essential oils on broiler performance

EO	Actual data				Percent increase (+) or decrease (−) VS NC			Age	References
	Dose rate (%)*	FI (g)	BWG (g)	FCR	FI	BWG	FCR
CEO	0 (control) PC1 0.01 0.02 0.03	4082ab 4125a 3973c 4013bc 4082ab	2122b 2311a 2169b 2219ab 2309a	1.92a 1.78b 1.83b 1.8b 1.76b	1.05 −2.67 −1.69 0	8.91 2.21 4.57 8.81	−7.29 −4.69 −6.25 −8.33	0–42	Ghazanfari et al., 2015
AjEO	0 (control) PC2 0.015 0.025 0.035	4317 4243 4170 4312 4323	2277b 2305ab 2329a 2288ab 2268b	1.89 1.84 1.79 1.88 1.9	−1.71 −3.41 −0.12 0.14	1.23 2.28 0.48 −0.4	−2.65 −5.29 −0.53 0.53	0–42	Falaki et al., 2016
AjEO	0 (control) PC3 0.04	3678 3721 3650	2188b 2304a 2164b	1.72a 1.65b 1.73a	1.17 −0.76	5.3 −1.10	−4.07 0.58	0–39	Chowdhury et al., 2018a
FEO	0 (control) 0.015 0.025	4773 4809 4896	2418 2484 2633	1.61 1.58 1.51	0.75 2.58	2.73 8.89	−1.86 −6.21	0–42	Gharehsheikhlou et al., 2018
FEO	0 (control) 0.0125 0.025	4633 4437 4517	2606 2537 2578	1.78 1.75 1.75	−4.23 −2.50	−2.65 −1.07	−1.69 −1.69	0–42	Stef et al., 2018
AnEO	0 (control) PC4 0.01 0.02 0.04	3450 3457 3433 3449 3470	2146c 2304b 2190c 2186c 2462a	1.61a 1.50b 1.57a 1.58a 1.41c	0.20 −0.49 −0.03 0.58	7.36 2.07 1.91 14.76	−6.83 −2.48 −1.86 −12.42	0–35	Ciftci et al., 2005
AnEO	0 (control) PC4 0.01 0.02 0.04	NA	2256c 2414b 2300c 2296c 2572a	NA		7.00 1.95 1.77 14.04	NA	0–40	Simsek et al., 2007
AnEO	0 (control) 0.0125 0.025	4633a 4326b 4302b	2606 2690 2672	1.78a 1.61b 1.61b	−6.63 −7.14	3.22 2.53	−9.55 −9.55	0–42	Stef et al., 2018
AnEO	0 (control) PC5 0.015 0.025 0.04	3479d 3719b 3491c 3545c 3852a	1697d 1937b 1828c 1846c 2105a	2.05a 1.92b 1.91b 1.92b 1.83c	6.90 0.34 1.90 10.72	14.14 7.72 8.78 23.98	−6.34 −6.83 −6.34 −10.73	0–42	Eltazi, 2014
AnEO	0 (control) PC6 0.01 0.02 0.04 0.06	3394 3437 3408 3412 3471 3450	1796d 1835bc 1809cd 1825bcd 1883a 1847b	1.97 1.95 1.96 1.95 1.92 1.94	1.27 0.41 0.53 2.27 1.65	2.17 0.72 1.61 4.84 2.84	−1.02 −0.51 −1.02 −2.54 −1.52	0–35	Bhandari and Yadav, 2013

PC=positive control

All values are in percentage except PC.

Flavophospholipol, 600 mg/kg

Virginiamycin, 200 mg/kg

Bacitracin methylene disalicylate, 500 mg/kg

Avilamycin, 1000 mg/kg

Neomycin sulfate, 1000 mg/kg

Cholotetracycline, 5 mg/kg

Mean values sharing a common superscript letter are not statistically different at P<0.05.

Table 7.

Effects of selected essential oils on carcass characteristics

EO	dietary dose %*	Hot dressing	Breast	Thigh	Wing	Gizzard	Liver	Heart	Abdominal fat	Spleen	Bursa	Age	Reference
		% of slaughter body weight
AjEO	0 (control) PC1 0.04	65.5 66.0 66.6	22.2 21.0 22.4	8.73 8.99 9.65	5.30 5.61 5.79	2.27 2.24 2.22	1.71 1.78 1.77	0.48 0.48 0.48	1.71 2.11 2.15	0.11ab 0.13a 0.10b	0.11 0.08 0.07	0–39	Chowdhury et al., 2018a b
AnEO	0 (control) PC4 0.015 0.025 0.040	67.5c 68.8b 68.7b 68.8b 69.1a	24.6c 25.8b 25.0b 25.5b 26.5a	15.0c 15.8b 15.8b 15.9b 16.8a	NA							0–42	Eltazi, 2014
AjEO	dietary dose %*				% of live body weight
	0 (control) PC2 0.015 0.025 0.035	63.8 65.3 66.0 64.6 64.0	19.9 21.8 21.2 21.4 20.0	17.4 18.1 18.2 17.6 17.8	NA	1.95 1.86 1.86 1.88 1.89	2.61 2.57 2.61 2.47 2.44	0.65 0.59 0.58 0.6 0.69	1.78 1.68 1.61 1.45 1.52	0.11 0.13 0.15 0.11 0.1	0.21 0.24 0.20 0.18 0.22	0–42	Falaki et al., 2016
AnEO	0 (control) PC3 0.01 0.02 0.04	73.7ab 72.9b 74.5ab 73.1ab 74.6a	28.5 29.0 28.8 28.7 29.5	22.2 21.31 21.36 21.11 21.46	10.8ab 10.7ab 11.3a 10.6ab 9.8b	2.06b 2.12bc 2.48ac 2.36abc 2.53a	2.4ab 2.27b 2.43ab 2.42ab 2.67a	0.51 0.51 0.49 0.47 0.41	2.34 2.45 2.44 2.62 2.75	0.13 0.14 0.14 0.13 0.12	NA	0–40	Simsek et al., 2007

PC=positive control

All values are in percentage except PC.

Bacitracin methylene disalicylate, 500 mg/kg

Neomycin sulfate, 1000 mg/kg

Virginiamycin, 200 mg/kg

Avilamycin, 1000 mg/kg

Mean values sharing a common superscript letter are not statistically different at P<0.05.

Table 8.

Effects of selected essential oils on serum traits

EO	Dietary dose %*	Cholesterol (mg/dl)	Triglyceride (mg/dl)	glucose (mg/dl)	HDL (mg/dl)	LDL (mg/dl)	VLDL (mg/dl)	Total Protein (mg/dl)	Age	Reference
CEO	0 (control) PC2 0.01 0.02 0.03	129 114 111 130 121	138 82 112 119 114	280 240 237 229 235	55 52 49 53 54	47 46 41 54 44	27 16 22 23 23	NA	0–42	Ghazanfari et al., 2015
AjEO	0 (control) PC1 0.04	184a 194a 148b	91 90 100	216 220 238	NA			2780 2660 2810	0–39	Chowdhury et al., 2018b

PC=positive control

All values are in percentage except PC.

Flavophospholipol, 600 mg/kg

Bacitracin methylene disalicylate, 500 mg/kg

Table 9.

Effects of selected essential oils on intestinal microbiota

EO	Intestine part	Dietary dose %*	Lactobacillus (log cfu/g)	E. coli (log cfu/g)	Clostridium (log cfu/g)	Age	Reference
CEO	Caecum content	0 (control) PC2 0.01 0.02 0.03	4.46 4.47 4.47 4.46 4.51	4.44a 4.23b 4.36ab 4.29b 4.25b	NA	0–42	Ghazanfari et al., 2015
AjEO	Pre-caecal digesta	0 (control) PC1 0.04	7.77 4.40 7.74	7.91a 7.29b 7.97a	7.27a 6.63b 7.26a	0–39	Chowdhury et al., 2018b

PC=positive control

All values are in percentage except PC.

Flavophospholipol, 600 mg/kg

Bacitracin methylene disalicylate, 500 mg/kg

Mean values sharing a common superscript letter are not statistically different at P<0.05.

Table 10.

Effects of selected essential oils on gut morphology

EO	Intestinal site	Dietary dose %*	VH		CD		VH/CD		Age	Reference
			(µm)	% change VS NC	(µm)	% change VS NC	Ratio	% change VS NC
CEO	Doudenum	0 (control) PC2 0.01 0.02 0.03	1759c 1912a 1798bc 1810b 1805b	8.7 2.2 2.9 2.6	147.8c 157.6ab 150.8bc 157.2ab 161.6a	6.6 2.0 6.4 9.3	11.91a 12.15a 11.94a 11.53ab 11.18b	2.0 0.3 −3.2 −6.1	0–42	Ghazanfari et al., 2015
	Jejunum	0 (control) PC2 0.01 0.02 0.03	849d 877a 858cd 866bc 872ab	3.3 1.1 2.0 2.7	107.6d 133.4a 109.4cd 114.2bc 116.0b	24.0 1.7 6.1 7.8	7.9a 6.58c 7.85ab 7.59ab 7.53b	−16.7 −0.6 −3.9 −4.7
	Ileum	0 (control) PC2 0.01 0.02 0.03	757c 829a 770bc 799ab 783bc	9.5 1.7 5.5 3.4	97d 129.8a 106.4c 116.2b 118.4b	33.8 9.7 19.8 22.0	7.88a 6.4c 7.26ab 6.89bc 6.61bc	−18.8 −7.9 −12.6 −16.1
AjEO	Doudenum	0 (control) PC1 0.04	1307. 1426 1230	9.1 −5.9	70.6 64.8 64.1	−8.2 −9.2	19.5b 22.9a 19.4b	17.4 −0.5	0–39	Chowdhury et al., 2018b
	Jejunum	0 (control) PC1 0.04	1070b 1261a 1036b	17.9 −3.2	63.7 67.5 69.0	6.0 8.3	17.0b 18.7a 15.9b	10 −6.5
	Ileum	0 (control) PC1 0.04	865b 1012a 959a	17.0 10.9	62.1b 68.4a 54.8b	10.1 −11.8	14.3b 14.5b 17.7a	1.4 23.8

PC=positive control

All values are in percentage except PC.

Flavophospholipol, (600 mg/kg)

Bacitracin methylene disalicylate, 500 mg/kg

Mean values sharing a common superscript letter are not statistically different at P<0.05.