Literature DB >> 3512552

Resolution of brewers' yeast pyruvate decarboxylase into two isozymes.

D J Kuo, G Dikdan, F Jordan.   

Abstract

A novel purification method was developed for brewers' yeast pyruvate decarboxylase (EC 4.1.1.1) that for the first time resolved the enzyme into two isozymes on DEAE-Sephadex chromatography. The isozymes were found to be distinct according to sodium dodecyl sulfate polyacrylamide gel electrophoresis: the first one to be eluted gave rise to one band, the second to two bands. The isozymes were virtually the same so far as specific activity, KM, inhibition kinetics and irreversible binding properties by the mechanism-based inhibitor (E)-4-(4-chlorophenyl)-2-oxo-3-butenoic acid are concerned. This finding resolves a longstanding controversy concerning the quaternary structure of this enzyme.

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Year:  1986        PMID： 3512552

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  Molecular cloning of the gene for indolepyruvate decarboxylase from Enterobacter cloacae.

Authors:  J Koga; T Adachi; H Hidaka
Journal:  Mol Gen Genet       Date:  1991-04

2.  Inactivation of pyruvate decarboxylase by 3-hydroxypyruvate.

Authors:  G Thomas; R Diefenbach; R G Duggleby
Journal:  Biochem J       Date:  1990-02-15       Impact factor: 3.857

3.  pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

Authors:  P G Seeboth; K Bohnsack; C P Hollenberg
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

Review 4.  Metabolic fuel utilization and pyruvate oxidation during the postnatal period.

Authors:  J M Medina; A Tabernero; J A Tovar; J Martín-Barrientos
Journal:  J Inherit Metab Dis       Date:  1996       Impact factor: 4.982

5.  The isolation and nucleotide sequence of the pyruvate decarboxylase gene from Kluyveromyces marxianus.

Authors:  P Holloway; R E Subden
Journal:  Curr Genet       Date:  1993-09       Impact factor: 3.886

6.  Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae.

Authors:  A P Wright; H L Png; B S Hartley
Journal:  Curr Genet       Date:  1989-03       Impact factor: 3.886

7.  Pyruvate decarboxylase from Zymomonas mobilis. Structure and re-activation of apoenzyme by the cofactors thiamin diphosphate and magnesium ion.

Authors:  R J Diefenbach; R G Duggleby
Journal:  Biochem J       Date:  1991-06-01       Impact factor: 3.857

8.  Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae.

Authors:  E Postma; C Verduyn; W A Scheffers; J P Van Dijken
Journal:  Appl Environ Microbiol       Date:  1989-02       Impact factor: 4.792

9.  A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations.

Authors:  I Schaaff; J B Green; D Gozalbo; S Hohmann
Journal:  Curr Genet       Date:  1989-02       Impact factor: 3.886

10.  Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli.

Authors:  A D Neale; R K Scopes; R E Wettenhall; N J Hoogenraad
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490
  10 in total

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