Fourier Deconvolution Ion Mobility Spectrometry.

Multiplexing the ion packet injection with advanced signal processing is an effective method to improve both the ion throughput and signal-to-noise ratio for ion mobility spectrometry. Generally used multiplexing methods include Hadamard transform ion mobility spectrometry (HT-IMS), Fourier transform ion mobility spectrometry (FT-IMS), and correlation ion mobility spectrometry (C-IMS). However, HT-IMS sometimes suffer from false peaks and further processing is needed, FT-IMS generally requires longer spectra acquisition time than the traditional signal averaging method, and C-IMS also demonstrated drawbacks such as spectra baseline distortions when using traditional on-off binary gating switches. To improve the performance of multiplexing ion mobility spectrometry, this study investigates the Fourier deconvolution to increase the resolving power and signal-to-noise ratio at the same time. This approach modulates the ion gate with a linear square wave chirp sequence and synchronizes the data acquisition and ion gate modulation and then reconstructs the ion mobility spectra based on convolution theorems. The equivalent ion injection period is decreased to microseconds scale with the signal-to-noise ratio improved by up to 13 times on average, and the resolving power is improved by up to 50% compared with traditional signal averaging methods without hardware modifications.