Nahomi Matsumura1,2, Xianqi Li1,2,3, Eri Uchikawa-Kitaya1,2, Ni Li1,4, Hongwei Dong1, Kai Chen1,5, Michiko Yoshizawa1,2, Hideaki Kagami6,7,8. 1. Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, 399-0781, Japan. 2. Department of Oral and Maxillofacial Surgery, School of Dentistry, Matsumoto Dental University, Shiojiri, 399-0781, Japan. 3. Division of Hard Tissue Research, Institute of Oral Science, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri, 399-0781, Japan. 4. Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai, 200031, China. 5. Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. 6. Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, 399-0781, Japan. hideaki.kagami@mdu.ac.jp. 7. Division of Hard Tissue Research, Institute of Oral Science, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri, 399-0781, Japan. hideaki.kagami@mdu.ac.jp. 8. Department of General Medicine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-9639, Japan. hideaki.kagami@mdu.ac.jp.
Abstract
BACKGROUND: Although tooth transplantation is a desirable treatment option for congenital defects of permanent teeth in children, transplantation to a narrow alveolar ridge is not feasible. In this study, we investigated the possibility of bone tissue engineering simultaneously with tooth transplantation to enhance the width of the alveolar bone. METHODS: Bone marrow mononuclear cells or cortical bone-derived mesenchymal stromal cell spheroids were seeded onto atelocollagen sponge and transplanted with freshly extracted molars from mice of the same strain. New bone formation around the tooth root was evaluated using micro-computed tomography and histological analysis. Tooth alone, or tooth with scaffold but without cells, was also transplanted and served as controls. RESULTS: Micro-computed tomography showed new bone formation in the furcation area in all four groups. Remarkable bone formation outside the root was also observed in the cortical bone-derived mesenchymal stromal cell group, but was scarce in the other three groups. Histological analysis revealed that the space between the new bone and the root was filled with collagen fibers in all four groups, indicating that the periodontal ligament was maintained. CONCLUSION: This study demonstrates the potential of simultaneous alveolar bone expansion employing bone tissue engineering approach using cortical bone-derived mesenchymal stromal cell spheroids for tooth transplantation. The use of an orthotopic transplantation model may further clarify the feasibility and functional recovery of the transplanted tooth over a longer period.
BACKGROUND: Although tooth transplantation is a desirable treatment option for congenital defects of permanent teeth in children, transplantation to a narrow alveolar ridge is not feasible. In this study, we investigated the possibility of bone tissue engineering simultaneously with tooth transplantation to enhance the width of the alveolar bone. METHODS: Bone marrow mononuclear cells or cortical bone-derived mesenchymal stromal cell spheroids were seeded onto atelocollagen sponge and transplanted with freshly extracted molars from mice of the same strain. New bone formation around the tooth root was evaluated using micro-computed tomography and histological analysis. Tooth alone, or tooth with scaffold but without cells, was also transplanted and served as controls. RESULTS: Micro-computed tomography showed new bone formation in the furcation area in all four groups. Remarkable bone formation outside the root was also observed in the cortical bone-derived mesenchymal stromal cell group, but was scarce in the other three groups. Histological analysis revealed that the space between the new bone and the root was filled with collagen fibers in all four groups, indicating that the periodontal ligament was maintained. CONCLUSION: This study demonstrates the potential of simultaneous alveolar bone expansion employing bone tissue engineering approach using cortical bone-derived mesenchymal stromal cell spheroids for tooth transplantation. The use of an orthotopic transplantation model may further clarify the feasibility and functional recovery of the transplanted tooth over a longer period.
Authors: Y Zhang; X Li; T Chihara; T Mizoguchi; A Hori; N Udagawa; H Nakamura; H Hasegawa; A Taguchi; A Shinohara; H Kagami Journal: Oral Dis Date: 2015-03-30 Impact factor: 3.511
Authors: Dirk Henrich; René Verboket; Alexander Schaible; Kerstin Kontradowitz; Elsie Oppermann; Jan C Brune; Christoph Nau; Simon Meier; Halvard Bonig; Ingo Marzi; Caroline Seebach Journal: Biomed Res Int Date: 2015-02-23 Impact factor: 3.411
Authors: Jonathan Hoggatt; Khalid S Mohammad; Pratibha Singh; Amber F Hoggatt; Brahmananda R Chitteti; Jennifer M Speth; Peirong Hu; Bradley A Poteat; Kayla N Stilger; Francesca Ferraro; Lev Silberstein; Frankie K Wong; Sherif S Farag; Magdalena Czader; Ginger L Milne; Richard M Breyer; Carlos H Serezani; David T Scadden; Theresa A Guise; Edward F Srour; Louis M Pelus Journal: Nature Date: 2013-03-13 Impact factor: 49.962