Serum miR-27a is a biomarker for the prognosis of non-small cell lung cancer patients receiving chemotherapy.

BACKGROUND: Lung cancer has a high incidence and a 5-year survival rate of less than 15%. Non-small cell lung cancer (NSCLC) accounts for approximately 85% of lung cancer cases. Chemotherapy and immunotherapy are the most frequently used alternative treatments for patients with advanced-stage NSCLC in whom surgery failed. Previous studies have suggested that miR-27a is involved in cancer development and progression. The purpose of this study was to investigate the clinical value of miR-27a in the prognosis of NSCLC patients after chemotherapy.
METHODS: Flow cytometry was used to detect the apoptosis rate of SPC-A1 cells treated with optical cisplatin at different times. Simultaneously, the expression of miR-27a in supernatants and cells was detected. Fifty-two newly diagnosed NSCLC patients were recruited. All patients received gemcitabine and cisplatin as first-line chemotherapy and docetaxel as second-line chemotherapy. At the end of every chemotherapy cycle, a therapeutic evaluation was performed according to the RECIST criteria. The expression of serum miR-27a was detected in each cycle.
RESULTS: After treatment with 2.5 µg/mL cisplatin, the apoptosis rates of SPC-A1 cells were significantly greater than those of the paired untreated control groups at 12, 24, 48 and 72 h. The expression of miR-27a in supernatants and cells was also consistent with the apoptosis rate and changed a time-dependent manner. The chi-square test showed that an increase in miR-27a after chemotherapy was more common in patients who achieved partial response (PR) than in those who achieved no response (NR) (61.5% vs. 30.8%, P=0.026). Kaplan-Meier survival analysis indicated that patients with decreased miR-27a levels had poorer outcomes than those with increased miR-27a levels (P<0.05). Furthermore, dynamic changes in serum miR-27a with a gradual increasing trend during chemotherapy predicted a good prognosis.
CONCLUSIONS: Collectively, our results suggest that miR-27a is involved in the apoptosis of lung cancer cells and that serum miR-27a levels are related to the prognosis of NSCLC patients. The expression levels of miR-27a in the serum may be an independent predictor for the prognosis of NSCLC. 2021 Translational Cancer Research. All rights reserved.

Introduction

Lung cancer affects the respiratory system and has a high incidence and a 5-year survival rate less than 15%. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and accounts for approximately 85% of lung cancer cases (1,2). Surgery is the most effective treatment for patients with early-stage NSCLC. However, most patients with advanced-stage lung cancer do not undergo surgery due to the low sensitivity and specificity of diagnostic methods (3). Currently, the most frequently used alternative treatments for NSCLC patients are chemotherapy and immunotherapy, which can help prolong overall survival and improve quality of life (4). Although chemotherapeutic agents, including cisplatin, are able to greatly improve the life quality of patients with advanced NSCLC (5), the prognosis of patients remains unsatisfactory. Thus, it is necessary to identify the specific survival markers of advanced NSCLC, which assist to reflect the therapeutic efficacy of chemotherapeutic agents and the prognosis.

MicroRNAs (miRNAs) are RNAs that are 19–25 nucleotide in length and have been widely recognized as key mediators in the epigenetic control of gene expression at the post-transcriptional level by base pairing to complementary sites on the 3'-untranslated region (3'-UTR) of target messenger RNAs (mRNAs) (6). They play an important regulatory role in a series of biological and pathological processes, such as development, differentiation, proliferation and apoptosis (7-10). Similar to the classification of genes, miRNAs can also be divided into onco-miRNAs and tumour suppressor miRNAs.

Previously, our research identified a monoclonal antibody called NJ001, whose function is specific to NSCLC (11). In this earlier study, we found that some miRNAs, including miR-638 and miR-27a, exhibited the most significant time-dependent expression in response to NJ001 (GEO accession number: GSE51947). Subsequently, we reported that the serum miR-638 level may be considered an emerging independent predictor for the prognosis of NSCLC because it is related to the survival of NSCLC patients (12). Moreover, recent publications have demonstrated that miR-27a, as a tumour suppressor miRNA, plays a vital role in the progression of cancers and influences tumourigenesis, tumour cell proliferation, apoptosis, invasion, and migration and angiogenesis (13-16). These results suggest that miR-27a is involved in the occurrence and progression of cancer. However, previous studies have rarely reported the prognostic value of miR-27a in advanced NSCLC. In this study, we identified the prognostic potential of miR-27a, which provides a theoretical foundation for the clinical application of miR-27a in NSCLC.

We present the following article in accordance with the STROBE reporting checklist (available at http://dx.doi.org/10.21037/tcr-20-3276).

Methods

Collection of cultured supernatants and SPC-A1 cells for in vitro experiments

The human lung adenocarcinoma cell line SPC-A1 was purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. The SPC-A1 cell line was not contaminated with mycoplasma or bacteria according to mass spectrometry results from the European Cell Culture Preservation Center. SPC-A1 cells were plated into six-well plates (1.5×106 per well) and treated with complete RPMI 1640 medium alone (as the control group) or 2.5 µg/mL cisplatin and incubated at 37 °C and 5% CO2 for 12, 24, 48 or 72 h. The optical treatment concentrations for cisplatin were based on our previous research (12). Harvested cultured supernatants and SPC-A1 cells were used for miR-27a detection. The whole experiment was repeated three times, and the mean of three time points were used.

Detection of the apoptosis rates of SPC-A1 cells

SPC-A1 cells (1×106) were collected, and the apoptosis rate was determined by flow cytometry. According to the manufacturer’s instructions, SPC-A1 cells were stained with 5 µL propidium iodide and 5 µL annexin V-FITC (Annexin V-FITC Apoptosis Detection Kit, BD Biosciences, Franklin, NJ, USA). All analyses were performed on a flow cytometer (BD Biosciences).

NSCLC patients and serum specimens

Fifty-two patients diagnosed with advanced NSCLC between March 2010 and May 2012 at the First Affiliated Hospital of Nanjing Medical University were recruited [32 males and 20 females, aged 31–77 years (median age: 61 years)]. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). All patients with newly diagnosed lung adenocarcinoma were unable to undergo radical surgery and did not receive radiotherapy or chemotherapy. The diagnosis of lung cancer was confirmed by a histopathological examination. All patients received gemcitabine and cisplatin as first-line chemotherapy and docetaxel as second-line chemotherapy. At the end of every chemotherapy cycle, a therapeutic evaluation was performed according to the RECIST criteria and included complete response (CR), partial response (PR), stable disease (SD) and progressive disease (PD). SD and PD belong to the no response (NR) category. Patients were followed until March 2013. The present study was approved by the Research Ethics Committee of the First Affiliated Hospital of Nanjing Medical University (No. 2015-SRFA-065, Nanjing, China), and all patients provided written consent prior to study enrolment. After obtaining informed consent, blood was drawn before and after chemotherapy. Whole blood was centrifuged at 2,500 g for 10 min, followed by 16,000 g for 10 min to completely remove cell debris. Then, the serum was separated, aliquoted and stored at –70 °C.

RNA extraction

According to the manufacturer’s protocol, total cell supernatants and serum RNA were extracted for quantitative RT-PCR (qRT-PCR) using the miRNeasy Mini Kit (QIAgen, Düsseldorf, Germany). cel-miR-39 was added as an internal control (59-ucaccggguguaaaucagcuug-39; final concentration: 10–5 pmol/mL). Total RNA was extracted from cells using QIAzol Lysis Reagent (QIAgen, Düsseldorf, Germany) according to the manufacturer’s protocol. A UV spectrophotometer (at 260 and 280 nm) and agarose gel electrophoresis were used to validate the RNA concentration and quality.

qRT-PCR

The miR-27a expression levels in the supernatants, cells and serum were detected by qRT-PCR. According to the manufacturer’s instructions, 5.84 µL of reverse transcription (RT) reagent (Applied Biosystems, Foster City, CA, USA), including stem-loop RT primers, was mixed with 9.16 µL of RNA in each reaction. RT reactions proceeded as follows: 16 °C for 30 min, followed by 42 °C for 30 min, 85 °C for 5 min and then maintained at 4 °C. Then, PCR was performed on the ABI 7500 real-time system as follows: 10 min at 95 °C, 15 s at 95 °C with 45 cycles and 1 min at 60 °C. The expression level of miR-27a in the serum is expressed as multiple changes based on the normalized expression level of cel-miR-39. The 2–ΔCt formula was used, in which ΔCt = CtmiR-27a – CtmiR-39. The expression level of miR-27a in the supernatants is expressed as multiple changes based on the normalized expression level of cel-miR-39. The 2–ΔΔCt formula was used, in which ΔΔCt = (CtmiR-27a – CtmiR-39)treated – (CtmiR-27a – CtmiR-39)untreated. The expression level of miR-27a in cells is expressed as multiple changes based on the normalized expression level of U6 snRNA. The 2–ΔΔCt formula was used, in which ΔΔCt = (CtmiR-27a – CtU6)treated – (CtmiR-27a – CtU6)untreated.

Statistical analysis

SPSS 16.0 software (Chicago, IL, USA) and GraphPad Prism 7.0 (San Diego, CA, USA) were used for statistical analyses. The proportions of miR-27a expression levels altered before and after chemotherapy between the PR and NR (including PD and SD) populations were compared using the chi-square test. The Kaplan-Meier method was used for survival analysis. We compared survival times between groups with the log-rank test. A P value <0.05 was considered statistically significant.

Results

Apoptosis of SPC-A1 cells induced by cisplatin

SPC-A1 cells were treated with 2.5 µg/mL cisplatin at different times to evaluate the effect of cisplatin on apoptosis. Flow cytometry was used to detect the apoptosis rate at 12, 24, 48 and 72 h after treatment with or without cisplatin. Compared with the paired untreated control cells, the morphology of SPC-A1 cells was changed (). Moreover, the apoptosis rates of the SPC-A1 cells cultured for 12, 24, 48 and 72 h with cisplatin were significantly higher than those without cisplatin (). With prolonged cisplatin exposure, these apoptosis rates increased more markedly. These results suggest that cisplatin induces apoptosis in SPC-A1 cells in a time-dependent manner.

Figure 1

Morphological changes in SPC-A1 cells treated with 2.5 µg/mL cisplatin were observed under a microscope (magnification, ×200). (A) Blank control; (B) 12 h; (C) 24 h; (D) 48 h; (E) 72 h.

Figure 2

Original flow cytometry plot of SPC-A1 cells at different time points post-treatment with 2.5 µg/mL cisplatin.

Figure 3

Apoptosis rates of SPC-A1 cells at different time points post-treatment with 2.5 µg/mL cisplatin (*, P<0.05). Apoptosis rates of control groups are 2.63±0.13, 2.87±0.10, 2.53±0.27, 2.65±0.07 after 12, 24, 48 and 72 h, respectively. Apoptosis rates of treatment groups are 4.47±0.11, 5.03±0.17, 57.53±1.99, 76.29±0.79 (*, P<0.05).

miR-27a expression in the supernatant of SPC-A1 cells increased in a time-dependent manner after cisplatin treatment

qRT-PCR was used to detect the expression level of miR-27a in the supernatant of SPC-A1 cells treated with cisplatin. We found that the miR-27a levels in the SPC-A1 cultured supernatants after treatment with 2.5 µg/mL cisplatin for 72 h were more than four-fold higher than those without treatment (). miR-27a expression levels in the treated group (2.5 µg/mL cisplatin) increased gradually at 12, 24, 48 and 72 h, and the difference between any period of the two groups was significant (P<0.05). When 2.5 µg/mL cisplatin was used for treatment for different time periods (12–72 h), the levels of miR-27a in supernatants increased in a time-dependent manner ().

Figure 4

Relative levels of miR-27a expression in SPC-A1 cultured supernatants and cells treated with 2.5 µg/mL cisplatin. (A) Cultured supernatants; (B) SPC-A1 cells (*, P<0.05).

miR-27a expression in SPC-A1 cells increased in a time-dependent manner after cisplatin treatment

qRT-PCR was used to determine the expression levels of miR-27a in SPC-A1 cells treated with cisplatin. The expression levels of miR-27a in SPC-A1 cells treated with 2.5 µg/mL cisplatin for 72 h were more than 8 times higher than those without cisplatin (). The miR-27a expression levels of SPC-A1 cells in the cisplatin (2.5 µg/mL) group increased between 12 and 24 h, and the miR-27a expression levels between the two groups were significantly different (P<0.05). However, there was no significant change at 24, 48 or 72 h. This result showed that the miR-27a expression levels in the cisplatin (2.5 µg/mL) group entered a platform after 24 h ().

Correlation between prognosis and the change in miR-27a expression levels before and after chemotherapy in NSCLC patients

As demonstrated in this study, accompanying the apoptosis of SPC-A1 cells, miR-27a expression levels also increased in SPC-A1 culture supernatants in a time-dependent manner after cisplatin treatment. Therefore, it is necessary to assess the prognostic value of NSCLC patients who receive first-line chemotherapy. The miR-27a expression levels in the sera of 52 patients receiving first-line chemotherapy were monitored before and after chemotherapy. The changes in miR-27a expression levels included an increase in 24 patients and a decrease in 28 patients. The follow-up was conducted until March 2013. The outcomes of the patients included 26 patients with PR and 26 patients with NR (including 10 patients with SD and 16 patients with PD). The detailed information is shown in . The chi-square test showed that a significant elevation of miR-27a after chemotherapy was more common in patients who achieved PR than in patients who achieved NR (61.5% vs. 30.8%, P=0.026). These results showed that the increase in the serum miR-27a levels after chemotherapy was related to a good curative effect of NSCLC patients.

Table 1

Correlation between prognosis and the change in miR-27a expression levels before and after chemotherapy in lung adenocarcinoma patients (n=52)

Prognosis	miR-27a change after chemotherapy		P value
	Increasing	Decreasing
PR	16	10	0.026
NR (SD + PD)	8	18

PR, partial response; NR, no response; SD, stable disease; PD, progressive disease.

Correlation between miR-27a levels and NSCLC patient survival

At the end of follow-up, 52 patients who completed first-line chemotherapy were divided into two groups according to the increase or decrease in serum miR-27a after chemotherapy. Among them, the serum miR-27a levels of 24 patients were increased, and those of 28 patients were decreased. Kaplan-Meier survival analysis showed that the average survival time of 24 patients with increased serum miR-27a after first-line chemotherapy was 28.4 months, while that of 28 patients with decreased serum miR-27a was 9 months. The survival time in the miR-27a increased group was significantly longer than that in the miR-27a decreased group (P<0.05). As shown in , the results showed that patients with decreased miR-27a levels had poorer prognoses than those with increased miR-27a levels.

Figure 5

Kaplan-Meier survival curve for the overall survival of NSCLC patients (n=52). Kaplan-Meier analysis was performed to evaluate the overall survival of NSCLC patients according to miR-27a expression patterns after one cycle of first-line chemotherapy: 24 patients had increasing miR-27a expression levels, and 28 patients had decreasing miR-27a expression levels.

Dynamic changes in serum miR-27a levels during chemotherapy

The dynamic changes in serum miR-27a were monitored in four patients with NSCLC after different chemotherapy cycles. As shown in , patient A received three cycles of first-line chemotherapy. Computed tomography (CT) showed that the tumour was significantly reduced, while tumour markers decreased and serum miR-27a levels increased gradually. At the end of follow-up, the patient survived and achieved PR. Patient B received three cycles of first-line chemotherapy, but due to the progression of multiple metastases throughout the body, 10 months later, the relative content of serum miR-27a levels continued to decrease during chemotherapy. After one cycle of first-line chemotherapy, disease progression resulted in a switch to second-line chemotherapy in patient C. After three cycles of second-line chemotherapy, the relative content of serum miR-27a levels continued to increase. By the end of follow-up, the patient achieved PR. Patient D received two cycles of first-line drug chemotherapy, after which his/her condition became stable, and the patient was discharged. After 1 month, the disease progressed, and the relative content of serum miR-27a levels was significantly decreased. Second-line chemotherapy was used instead of first-line chemotherapy, but the patient died after 11 months. The above four patients also demonstrated that miR-27a can be used as a tumour marker to assess individualized treatment efficacy during chemotherapy.

Figure 6

Dynamic changes in miR-27a levels at different time points following chemotherapy.

Discussion

NSCLC is the most-common subtype of lung cancer, mainly including adenocarcinoma, squamous cell carcinoma and large cell lung cancer (17). Recent studies have found that some novel surgical methods like uniportal video-assisted thoracoscopic surgery and robotic-assisted thoracoscopic surgery have superb advantages over open surgery, which significantly reduces pain, complications, inflammatory response and improves postoperative life quality (18). However, a large proportion of NSCLC patients cannot benefit from these novel surgical methods. An accurate evaluation of the disease condition is conductive to prolong overall survival and effectively reduce the risk of surgical therapies of NSCLC. It is well known that the treatment of NSCLC is stage-specific. Most of patients with advanced NSCLC should be considered for chemotherapy, but their prognosis is still very poor. Therefore, reliable biomarkers are urgently needed for predicting the prognosis of patients with advanced NSCLC receiving chemotherapy.

miRNAs have been investigated for several decades and can negatively regulate gene expression by recognizing the 3'-UTR of a specific target mRNA and inhibiting translation at the post-transcriptional level (19). Recent studies have revealed that miRNAs affect the biological behaviour of lung tumours, and miRNA detection has been widely used for early diagnosis (20), prognosis (21) and therapeutic (22) evaluations, as well as chemosensitivity (23) and chemoresistance (24) evaluations.

Recently, miRNAs have been widely studied as an important means of epigenetic regulation (25). A considerable number of studies have shown that the dysregulation of miRNAs may be involved in the occurrence and development of NSCLC. Therefore, miRNAs could serve as promising molecular markers for clinical application (26). For example, Pan et al. revealed that miR-33a-5p and miR-128-3p were more sensitive/specific than traditional tumour markers such as NSE, CYFRA21-1 and CA72-4 and could serve as novel biomarkers for the early detection of lung cancer (27). Yang et al. indicated that miR-21 and miR-155 showed promising associations with the prognosis of lung cancer and could predict recurrence and poor survival in NSCLC patients (28). The miR-27 family is composed of miR-27a and miR-27b, and miR-27a plays an important role in tumour development. According to recent studies, miR-27a is significantly dysregulated in various cancers, such as liver cancer (29), NSCLC (30) and prostate cancer (31), and acts as an oncogene. miR-27a also functions as a tumour suppressor in bladder cancer (32), gastric cancer (33), and oesophageal squamous cell cancer (34). These contradictory results have prompted us to investigate the role of miR-27a in tumours and its potential clinical applications.

In a previous miRNA expression microarray analysis, we found that the expression levels of miR-27a in SPC-A1 cells treated with NJ001 increased in a time-dependent manner. To further discover the clinical application of miR-27a in NSCLC, the apoptosis rates of SPC-A1 cells treated with 2.5 µg/mL cisplatin for different periods in vitro were measured. Simultaneously, miR-27a expression levels in culture supernatants and cells were measured. Our results suggested that SPC-A1 cell apoptosis was induced by cisplatin in a time-dependent manner, consistent with the research of Shi et al. (35). Importantly, the apoptosis rates were positively associated with the increased expression of miR-27a in a time-dependent manner in both SPC-A1 cells and culture supernatants. The expression of miR-27a increased with prolonged exposure and apoptosis rates, suggesting that miR-27a plays an active role in apoptosis.

The serum miR-27a levels of 52 patients with lung adenocarcinoma were also measured before and after chemotherapy by qRT-PCR analysis. We found that the proportion of patients with elevated serum miR-27a levels was 61.5% in the PR group and 30.8% in the NR group after the first chemotherapy cycle. This result suggested that increased serum miR-27a levels after chemotherapy were correlated with the prognosis of NSCLC patients. Furthermore, Kaplan-Meier survival analysis showed that after first-line chemotherapy, the average survival times of patients with increased and decreased serum miR-27a levels were 28.4 vs. 9 months, respectively. These results suggested that patients with decreased miR-27a levels had poorer outcomes than those with increased miR-27a levels.

Previous research has shown that dynamic changes in miRNAs can be used to discriminate the type of response to antitumour therapy in lung cancer patients (36), and circulating tumour cells (CTCs) counts can be used for the dynamic monitoring and prediction of outcomes in patients with epidermal growth factor receptor (EGFR)-mutated NSCLC treated with EGFR-tyrosine kinase inhibitors (TKIs) (37). Inspired by these studies, we evaluated the dynamic changes in the expression of miR-27a in the serum of representative patients before and after chemotherapy. The results showed that the level of serum miR-27a in the PR group increased gradually (patient A and patient C), while that in the SD group and PD group decreased (patient B). Hence, these results revealed that dynamic changes in miR-27a levels in such individualized cases combined with therapeutic evaluations can be used to predict patient outcomes. A recent study has revealed that plasma level of miR-32 in NSCLC patients receiving platinum-based chemotherapy is considered as a prognostic indicator (38). Our findings showed that the elevated level of miR-27a in NSCLC patients receiving chemotherapy was able to predict the therapeutic efficacy of platinum-based chemotherapy and the prognosis. Nevertheless, a single biomarker may have limitations to predict the prognosis of NSCLC, and detecting a combination of prognostic biomarkers can be more reliable. We speculated that detecting both miR-27a miR-32 levels in patients with advanced NSCLC receiving chemotherapy is a promising approach to assist the evaluation of prognosis, which will be explored in future studies.

It is generally considered that genetic variation affects the chemotherapy response and overall survival of cancer patients. Because of gene variations, their therapeutic efficacy differs a lot in NSCLC patients receiving chemotherapy, with the chemotherapy response ranging from 26% to 60% (39). In particular, mutation subsets in NSCLC, including EGFR, anaplastic lymphoma kinase (ALK), c-ros oncogene 1 (ROS1) and v-raf murine viral oncogene homolog B1 (BRAF), are correlated with the sensitivity of chemotherapeutic agents or acquired resistance (40). Compared with these targets, miR-27a may serve as a promising marker for predicting the efficacy of chemotherapeutic agents and prognosis in NSCLC.

Liquid biopsy, which includes CTCs, circulating-free DNA (cfDNA), miRNAs, exosomes and tumour-educated platelets (TEPs), has potential use for non-invasive screening, early diagnosis, prognosis, response to treatment and real-time monitoring in NSCLC patients (41). Recently, many studies have demonstrated that a considerable number of miRNAs can be used as prognostic tools (42,43). miR-27a is a well-identified miRNA in tumours, and its diverse functions depend mainly on the cancer type, as it plays a role in either cancer promotion or tumour suppression (44). In our study, there was a positive correlation between the expression of miR-27a and the apoptosis of tumour cells. Moreover, patients with increased miR-27a levels after the first cycle of chemotherapy had better outcomes than patients with decreased miR-27a levels. These results confirm that miR-27a may play a role in tumour suppression in lung cancer progression, consistent with the results of Acunzo et al. (30) but contradictory to those of Chae et al. (45). These contradictory results need to be confirmed by further research.

Currently, drug resistance remains the main cause of chemotherapeutic treatment failure (46). Relevant studies have identified that nucleotide excision repair (NSR) gene variation plays a key role in chemotherapeutic drug resistance, which reduces the efficacy of chemotherapeutic drugs through DNA damage repair (47,48). Our findings have shown that miR27a levels can be used as a prognostic marker in NSCLC patients receiving chemotherapy. However, the mechanisms have not been fully revealed. Recent studies indicated that some miRNAs improve anti-cancer therapy by DNA damage repair (49). Therefore, we suspect that miR-27a can predict the sensitivity of chemotherapeutic drugs and the prognosis of patients by regulating NSR gene variation. For this, we will explore the relationship between miR-27a and NSR gene variation in NSCLC, which contributes to the individualized treatment of patients.

There are some limitations to be addressed in our study, such as a single-centre study with a small sample size. Therefore, the findings in this study will be investigated with multiple and large-scale samples in a wider population.

Conclusions

We found that the expression of miR-27a in the sera of NSCLC patients changed after the first chemotherapy cycle, and this change was related to the prognosis of NSCLC. More precisely, an increase in serum miR-27a is related to a good prognosis in NSCLC patients. Dynamic changes in serum miR-27a after each cycle of chemotherapy showing a gradual increase predict good clinical outcomes. Serum miR-27a may be a prognostic biomarker for NSCLC patients. However, the potential molecular mechanism of miR-27a in lung cancer and its therapeutic target for NSCLC need further study.