Pengfei Lu1, Jun Li2, Rui Mao1, Hongzhi Qi1, Liping Yang1, Qin Zhou1, Mengxiao Tian2, Wenbao Zhang3, Yongxing Bao4. 1. Department of Tumor Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 of Li Yushan Street, Xinshi District, Urumqi, 830011, China. 2. Department of Tumor Center, The First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, No. 137 of Li Yushan Street, Xinshi District, Urumqi, 830011, China. 3. Department of Tumor Center, The First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, No. 137 of Li Yushan Street, Xinshi District, Urumqi, 830011, China. wenbaozhang2013@163.com. 4. Department of Tumor Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 of Li Yushan Street, Xinshi District, Urumqi, 830011, China. Yongxing_Bao@21cn.com.
Abstract
PURPOSE: Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu lato (s.l.) is a globally distributed zoonosis. CE treatment is difficult, but radiation and 5-fluorouracil (5-FU) can be effective. However, the combination of radiation and 5-FU has not been reported. This study evaluated the effect of combination of 5-FU and radiation on E. granulosus s.l. protoscoleces (PSCs). MATERIAL AND METHODS: In this study, PSCs were collected from the liver of diseased sheep, and some were exposed to a single dose of 20 Gy 6-MV X-ray combined with (5 μg/mL or 10 μg/mL) 5-FU in vitro. Methylene blue staining was used to detect the viability of the PSCs. Transcription of EgHSP70 and Egp38 was measured by quantitative real-time PCR (qRT- PCR). RESULTS: A single dose of radiation killed 18% of the PSCs, and 5-FU showed weak parasiticidal efficacy on the first day of treatment. After 14 d, 5 μg and 10 μg/mL of 5-FU killed 40.20% and 50.02% of the PSCs, whereas 20 Gy of radiation killed 31.44%. The combination of 5-FU (10 μg/mL) with 20 Gy of radiation showed 77.55% killing efficacy. qRT-PCR showed that 5-FU inhibited Egp38 expression, whereas radiation increased its expression. EgHSP70 was highly expressed 14 days after radiation treatment. The data indicate that 5-FU has parasiticidal efficacy against the PSCs of E. granulosus s.l. CONCLUSION: The lethal efficacy of PSCs caused by a single dose of radiation exposure is related to the upregulated expression level of Egp38 and EgHSP70. The killing effect of 5-FU (10 μg/mL) with 20Gy of radiation was significantly better than that of single treatment group. This study provided a basis for the potential role of 5-FU combined with radiation in the treatment of CE.
PURPOSE: Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu lato (s.l.) is a globally distributed zoonosis. CE treatment is difficult, but radiation and 5-fluorouracil (5-FU) can be effective. However, the combination of radiation and 5-FU has not been reported. This study evaluated the effect of combination of 5-FU and radiation on E. granulosus s.l. protoscoleces (PSCs). MATERIAL AND METHODS: In this study, PSCs were collected from the liver of diseased sheep, and some were exposed to a single dose of 20 Gy 6-MV X-ray combined with (5 μg/mL or 10 μg/mL) 5-FU in vitro. Methylene blue staining was used to detect the viability of the PSCs. Transcription of EgHSP70 and Egp38 was measured by quantitative real-time PCR (qRT- PCR). RESULTS: A single dose of radiation killed 18% of the PSCs, and 5-FU showed weak parasiticidal efficacy on the first day of treatment. After 14 d, 5 μg and 10 μg/mL of 5-FU killed 40.20% and 50.02% of the PSCs, whereas 20 Gy of radiation killed 31.44%. The combination of 5-FU (10 μg/mL) with 20 Gy of radiation showed 77.55% killing efficacy. qRT-PCR showed that 5-FU inhibited Egp38 expression, whereas radiation increased its expression. EgHSP70 was highly expressed 14 days after radiation treatment. The data indicate that 5-FU has parasiticidal efficacy against the PSCs of E. granulosus s.l. CONCLUSION: The lethal efficacy of PSCs caused by a single dose of radiation exposure is related to the upregulated expression level of Egp38 and EgHSP70. The killing effect of 5-FU (10 μg/mL) with 20Gy of radiation was significantly better than that of single treatment group. This study provided a basis for the potential role of 5-FU combined with radiation in the treatment of CE.
Authors: G Cringoli; P Pepe; A Bosco; M P Maurelli; L Baldi; P Ciaramella; V Musella; M L Buonanno; F Capuano; F Corrado; D Ianniello; L C Alves; P Sarnelli; L Rinaldi Journal: Vet Parasitol Date: 2021-01-05 Impact factor: 2.738
Authors: Patricia E Pensel; Natalia Elissondo; Guillermo Gambino; Gabriela Ullio Gamboa; J P Benoit; María C Elissondo Journal: Vet Parasitol Date: 2017-08-19 Impact factor: 2.738
Authors: Rui Mao; Hongzhi Qi; Lei Pei; Jie Hao; Jian Dong; Tao Jiang; Abudula Ainiwaer; Ge Shang; Lin Xu; Xi Shou; Songan Zhang; Ge Wu; Pengfei Lu; Yongxing Bao; Haitao Li Journal: Biomed Res Int Date: 2017-08-31 Impact factor: 3.411