Literature DB >> 3511028

Cloning of genes that encode a new heat-labile enterotoxin of Escherichia coli.

C L Pickett, E M Twiddy, B W Belisle, R K Holmes.   

Abstract

The genes for a new enterotoxin were cloned from Escherichia coli SA53. The new toxin was heat labile and activated adenylate cyclase but was not neutralized by antisera against cholera toxin or E. coli heat-labile enterotoxin. Subcloning and minicell experiments indicated that the toxin is composed of two polypeptide subunits that are encoded by two genes. The two toxin subunits exhibited mobilities on polyacrylamide gels that are similar to those of cholera toxin and E. coli heat-labile enterotoxin subunits. A 0.8-kilobase DNA probe for the new enterotoxin failed to hybridize with the cloned structural genes for E. coli heat-labile enterotoxin.

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Year:  1986        PMID： 3511028      PMCID： PMC214422          DOI： 10.1128/jb.165.2.348-352.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  MINIATURE escherichia coli CELLS DEFICIENT IN DNA.

Authors:  H I Adler; W D Fisher; A Cohen; A A Hardigree
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

2.  Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.

Authors:  B Lugtenberg; J Meijers; R Peters; P van der Hoek; L van Alphen
Journal:  FEBS Lett       Date:  1975-10-15       Impact factor: 4.124

3.  Cistrons encoding Escherichia coli heat-labile toxin.

Authors:  W S Dallas; D M Gill; S Falkow
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

4.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

5.  Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants.

Authors:  J J Mekalanos; R J Collier; W R Romig
Journal:  Infect Immun       Date:  1978-05       Impact factor: 3.441

6.  The gene for type A streptococcal exotoxin (erythrogenic toxin) is located in bacteriophage T12.

Authors:  C R Weeks; J J Ferretti
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits.

Authors:  R K Holmes; E M Twiddy
Journal:  Infect Immun       Date:  1983-12       Impact factor: 3.441

9.  Effects of lincomycin and tetracycline on production and properties of enterotoxins of enterotoxigenic Escherichia coli.

Authors:  M Yoh; K Yamamoto; T Honda; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1983-11       Impact factor: 3.441

10.  Immunological nonidentity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Authors:  T Honda; T Tsuji; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1981-11       Impact factor: 3.441
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  36 in total

1.  Clonal structure and virulence factors in strains of Escherichia coli of the classic serogroup O55.

Authors:  J Rodrigues; I C Scaletsky; L C Campos; T A Gomes; T S Whittam; L R Trabulsi
Journal:  Infect Immun       Date:  1996-07       Impact factor: 3.441

2.  Virulence factors and biochemical characteristics of serotypes of Escherichia coli serogroup O29.

Authors:  B E Guth; R M Silva; M R Toledo; T M Lima; L R Trabulsi
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948

Review 3.  Animal Enterotoxigenic Escherichia coli.

Authors:  J Daniel Dubreuil; Richard E Isaacson; Dieter M Schifferli
Journal:  EcoSal Plus       Date:  2016-10

Review 4.  Resistance to ETEC F4/F18-mediated piglet diarrhoea: opening the gene black box.

Authors:  Rebeka Sinha; Nihar Ranjan Sahoo; Kush Shrivastava; Pushpendra Kumar; Salauddin Qureshi; Ujjwal Kumar De; Amit Kumar; Gandham Venkata Papa Pydi Siva Ravi Kumar; Bharat Bhushan
Journal:  Trop Anim Health Prod       Date:  2019-05-24       Impact factor: 1.559

Review 5.  Shiga and Shiga-like toxins.

Authors:  A D O'Brien; R K Holmes
Journal:  Microbiol Rev       Date:  1987-06

6.  Enterotoxin-based mucosal adjuvants alter antigen trafficking and induce inflammatory responses in the nasal tract.

Authors:  Frederik W van Ginkel; Raymond J Jackson; Naoto Yoshino; Yukari Hagiwara; Daniel J Metzger; Terry D Connell; Hong L Vu; Michael Martin; Kohtaro Fujihashi; Jerry R McGhee
Journal:  Infect Immun       Date:  2005-10       Impact factor: 3.441

7.  Virulence properties and clonal structures of strains of Escherichia coli O119 serotypes.

Authors:  A G Gonçalves; L C Campos; T A Gomes; J Rodrigues; V Sperandio; T S Whittam; L R Trabulsi
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

8.  Development of DNA probes for cytotoxin and enterotoxin genes in enteric bacteria.

Authors:  G M Brazil; C L Clayton; T Sekizaki; K N Timmis
Journal:  Experientia       Date:  1988-10-15

9.  A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka.

Authors:  A M Tokhi; J S Peiris; S M Scotland; G A Willshaw; H R Smith; T Cheasty
Journal:  Epidemiol Infect       Date:  1993-04       Impact factor: 2.451

10.  Virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of Escherichia coli.

Authors:  E Oswald; J de Rycke; P Lintermans; K van Muylem; J Mainil; G Daube; P Pohl
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948
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