Literature DB >> 3510966

Identification of trophoblast in chorionic villi biopsy samples.

A E Covone, P M Johnson.   

Abstract

We have investigated a test for rapid discrimination between foetal and maternal origin of chorionic villi biopsy samples. A monoclonal antibody named H315 reacting against a specific antigen present on the surface of foetal trophoblastic cells, plus a double-colour staining technique (FITC + PI), have been used for the identification of foetal cells (H315-positive) and for visualization of nucleate (PI-positive) and anucleate (PI-negative) structures of chorionic villi. This test could be useful in differentiating foetal and maternal cells in chorionic villi biopsy samples currently used for prenatal diagnostic purposes.

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Year:  1986        PMID： 3510966     DOI： 10.1007/bf00283940

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  7 in total

1.  A single-operator technique for first-trimester chorion biopsy.

Authors:  C H Rodeck; J M Morsman; K H Nicolaides; C McKenzie; C M Gosden; J R Gosden
Journal:  Lancet       Date:  1983-12-10       Impact factor: 79.321

Review 2.  First-trimester chorion biopsy.

Authors:  C H Rodeck; J M Morsman
Journal:  Br Med Bull       Date:  1983-10       Impact factor: 4.291

3.  Localization in human term placental bed and amniochorion of cells bearing trophoblast antigens identified by monoclonal antibodies.

Authors:  P M Johnson; C M Molloy
Journal:  Am J Reprod Immunol       Date:  1983 Jul-Aug       Impact factor: 3.886

4.  Efficient direct chromosome analyses and enzyme determinations from chorionic villi samples in the first trimester of pregnancy.

Authors:  G Simoni; B Brambati; C Danesino; F Rossella; G L Terzoli; M Ferrari; M Fraccaro
Journal:  Hum Genet       Date:  1983       Impact factor: 4.132

5.  Immunohistologic identification of trophoblast populations in early human pregnancy with the use of monoclonal antibodies.

Authors:  J N Bulmer; W D Billington; P M Johnson
Journal:  Am J Obstet Gynecol       Date:  1984-01-01       Impact factor: 8.661

6.  Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions. A new method for rapid isolation and straining of nuclei.

Authors:  L L Vindelov
Journal:  Virchows Arch B Cell Pathol       Date:  1977-08-10

7.  Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies.

Authors:  P J Brown; C M Molloy; P M Johnson
Journal:  J Reprod Immunol       Date:  1983-11       Impact factor: 4.054
  7 in total
  1 in total

1.  [Status of chorionic villi biopsy in West Germany].

Authors:  H Weitzel
Journal:  Arch Gynecol Obstet       Date:  1987       Impact factor: 2.344
  1 in total

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