| Literature DB >> 3510692 |
M Geffard, S Patel, J Dulluc, A M Rock.
Abstract
Noradrenaline (NA) was conjugated to different protein carriers with glutaraldehyde. During the catecholamine conjugate synthesis, precautions were taken in order to preserve the structure of NA. Anti-NA antibodies were raised in rabbits and tested using a modified enzyme-linked immunosorbent assay and immunocytochemistry. The most immunoreactive antigen was found to be the reduced NA conjugate. The octopamine conjugate was recognized by the NA antiserum 16 X less than the reduced NA conjugate. The other conjugates, dopamine, L-dihydroxyphenylalanine and adrenaline were poorly recognized by the anti-NA antibodies. A good correlation was found between results obtained using the in vitro enzyme-linked immunosorbent assay and immunocytochemistry.Entities:
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Year: 1986 PMID： 3510692 DOI： 10.1016/0006-8993(86)91031-0
Source DB: PubMed Journal: Brain Res ISSN： 0006-8993 Impact factor: 3.252