| Literature DB >> 35103976 |
Timothy M Johanson1,2, Rhys S Allan3,4.
Abstract
In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation sequenced to determine their linear genomic position, but also their three-dimensional relationship. Here, we describe these steps in detail.Entities:
Keywords: Chromatin architecture; Chromosome conformation capture; Genome organization; In situ HiC
Mesh：
Substances：
Year: 2022 PMID： 35103976 DOI： 10.1007/978-1-0716-2140-0_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745