Literature DB >> 35103961

Next-Generation Bisulfite Sequencing for Targeted DNA Methylation Analysis.

Jim Smith1, Robert C Day2, Robert J Weeks3.   

Abstract

Bisulfite sequencing is the "gold-standard" technique for DNA methylation analysis. By combining bisulfite sequencing with high-throughput, next-generation sequencing technology, we can document methylation from many thousands of individual reads (equivalent to alleles or "cells"), for multiple target regions and from many samples simultaneously. Here, we describe a next-generation bisulfite-sequencing assay for targeted DNA methylation analysis which offers scope for the simultaneous interrogation of multiple genomic loci across numerous samples.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Analysis; Bisulfite conversion; DNA methylation; Next-generation sequencing

Mesh：

Substances：

Year:  2022        PMID： 35103961     DOI： 10.1007/978-1-0716-2140-0_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

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3.  Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.

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Review 7.  Promoter DNA Hypermethylation and Paradoxical Gene Activation.

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Review 8.  The methylome: approaches for global DNA methylation profiling.

Authors:  Stephan Beck; Vardhman K Rakyan
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9.  Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms.

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Journal:  ISME J       Date:  2012-03-08       Impact factor: 10.302

10.  Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements.

Authors:  Marcel W Coolen; Aaron L Statham; Margaret Gardiner-Garden; Susan J Clark
Journal:  Nucleic Acids Res       Date:  2007-09-13       Impact factor: 16.971
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  1 in total

Review 1.  Enzyme-free targeted DNA demethylation using CRISPR-dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression.

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