Fatemeh Nezamzadeh1, Mahboobeh Asadyun2, Amir Anbiyaiee3, Mansour Sedighi4, Abed Zahedi Bialvaei5, Younes Khalili6, Hamed Ebrahimzadeh Leylabadlo7, Aylin Esmailkhani8. 1. MD, School of Medicine, Islamic Azad University, Tonekabon Branch, 5 kilometers to Tonekabon City - Vali Abad, Tonekabon, Iran. 2. MSc, Department of Biology, Science and Research Branch, Islamic Azad University, Shodada Hesarak blvd, Daneshgah Square, Tehran, Iran. 3. MD, Department of Obstetrics & Gynecology, Medical School, Jundishapur University of Medical Sciences, Golestan St., Ahvaz, Iran. 4. PhD, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Hemmat Highway, next to the Milad Tower, Tehran, Iran. 5. PhD, Microbial Biotechnology Research Center, Iran University of Medical Sciences, Hemmat Highway, next to the Milad Tower, Tehran, Iran. 6. PhD, Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Golgasht St., Tabriz, Iran. 7. PhD, Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Golgasht St., Tabriz, Iran. 8. MSc, Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Golgasht St., Tabriz, Iran.
Abstract
INTRODUCTION: The Helicobacter pylori infection and cytokine-mediated inflammatory responses play significant roles in the pathogenesis of gastric cancer (GC). This study was performed to determine the association between the risk of GC and genetic polymorphisms in interleukin (IL)-1β and tumor necrosis factor-alpha (TNF-α). METHODS: The polymorphisms of IL-1β and TNF-α genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 290 patients who underwent endoscopy. Infection with H. pylori was diagnosed by histological analysis, rapid urease test, and PCR of gastric biopsy samples. Quantitative real-time PCR was performed to determine the relative mRNA expression levels. RESULTS: No significant difference was detected in allele frequency and genotype of all studied polymorphisms between chronic gastritis (CG), GC and healthy individuals. IL-1β mRNA was down-regulated in both gastritis (relative quantification (RQ)=0.447) and the GC groups (RQ=0.151). In contrast, the expression of TNF-α was up-regulated in the GC group (RQ=2.817) compared to the gastritis group (RQ=0.861). CONCLUSIONS: The studied single-nucleotide polymorphisms are not risk factors for development of CG and GC. However, H. pylori infection causes a huge increase in the TNF-α expression in GC patients. GERMS.
INTRODUCTION: The Helicobacter pylori infection and cytokine-mediated inflammatory responses play significant roles in the pathogenesis of gastric cancer (GC). This study was performed to determine the association between the risk of GC and genetic polymorphisms in interleukin (IL)-1β and tumor necrosis factor-alpha (TNF-α). METHODS: The polymorphisms of IL-1β and TNF-α genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 290 patients who underwent endoscopy. Infection with H. pylori was diagnosed by histological analysis, rapid urease test, and PCR of gastric biopsy samples. Quantitative real-time PCR was performed to determine the relative mRNA expression levels. RESULTS: No significant difference was detected in allele frequency and genotype of all studied polymorphisms between chronic gastritis (CG), GC and healthy individuals. IL-1β mRNA was down-regulated in both gastritis (relative quantification (RQ)=0.447) and the GC groups (RQ=0.151). In contrast, the expression of TNF-α was up-regulated in the GC group (RQ=2.817) compared to the gastritis group (RQ=0.861). CONCLUSIONS: The studied single-nucleotide polymorphisms are not risk factors for development of CG and GC. However, H. pylori infection causes a huge increase in the TNF-α expression in GC patients. GERMS.
Authors: Aleksandra Sałagacka; Marta Żebrowska; Agnieszka Jeleń; Marek Mirowski; Ewa Balcerczak Journal: Gut Liver Date: 2014-11-15 Impact factor: 4.519