| Literature DB >> 35094330 |
Alejandro Stark1, Alexander Trick2, Thomas R Pisanic3, Tza-Huei Wang4.
Abstract
Early cancer detection requires identification of cellular changes resulting from oncogenesis. Abnormal DNA methylation patterns occurring early in tumor development have been widely identified as early biomarkers for multiple types of cancer tumors. Methylation-Specific PCR (MSP) has permitted highly sensitive detection of these methylation changes at known biomarker locations. MSP requires multiple sample preparation steps including protein digestion, DNA isolation, and bisulfite conversion prior to detection. In this work, we present a streamlined assay platform and instrumentation for integration of all sample processing steps required to obtain quantitative MSP signal from raw biological samples through the use of droplet magnetofluidic principles. In conjunction with this platform, we present a streamlined protocol for solid-phase DNA extraction from cells and bisulfite conversion of genomic DNA, minimizing the processing steps and reagent volume for implementation on a compact assay platform.Entities:
Keywords: Biomarkers; Epigenetics; Lab-on-chip; Magnetofluidics; Methylation; Methylation-specific PCR; qPCR
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Year: 2022 PMID： 35094330 DOI： 10.1007/978-1-0716-1811-0_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745