Literature DB >> 35089577

Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.

Romana Köszagová1,2, Eva Hrabárová1,2, Lucia Achbergerová1,2, Jozef Nahálka3,4.   

Abstract

Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (D-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase, UDP-glucose pyrophosphorylase) physiologically aggregated within inclusion bodies retaining their biological activity as immobilized biocatalysts.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Biocatalysis/biotransformation; Enzyme immobilization; Inclusion bodies; Recombinant enzymes

Mesh：

Substances：

Year:  2022        PMID： 35089577     DOI： 10.1007/978-1-0716-1859-2_30

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused.

Authors:  R B Kapust; D S Waugh
Journal:  Protein Sci       Date:  1999-08       Impact factor: 6.725

Review 2.  UDP-glucose pyrophosphorylase. An old protein with new tricks.

Authors:  Leszek A Kleczkowski; Matt Geisler; Iwona Ciereszko; Henrik Johansson
Journal:  Plant Physiol       Date:  2004-03       Impact factor: 8.340

3.  Physiological aggregation of maltodextrin phosphorylase from Pyrococcus furiosus and its application in a process of batch starch degradation to alpha-D-glucose-1-phosphate.

Authors:  Jozef Nahálka
Journal:  J Ind Microbiol Biotechnol       Date:  2007-12-18       Impact factor: 3.346

4.  A crosslinked inclusion body process for sialic acid synthesis.

Authors:  Jozef Nahálka; Alica Vikartovská; Eva Hrabárová
Journal:  J Biotechnol       Date:  2008-03-20       Impact factor: 3.307

Review 5.  Immobilised enzymes: carrier-bound or carrier-free?

Authors:  Linqiu Cao; Luuk van Langen; Roger A Sheldon
Journal:  Curr Opin Biotechnol       Date:  2003-08       Impact factor: 9.740

Review 6.  Molecular chaperones in protein folding and proteostasis.

Authors:  F Ulrich Hartl; Andreas Bracher; Manajit Hayer-Hartl
Journal:  Nature       Date:  2011-07-20       Impact factor: 49.962

Review 7.  Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology.

Authors:  Pietro Gatti-Lafranconi; Antonino Natalello; Diletta Ami; Silvia Maria Doglia; Marina Lotti
Journal:  FEBS J       Date:  2011-05-31       Impact factor: 5.542

8.  Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3).

Authors:  Jozef Nahálka; Vladimír Pätoprstý
Journal:  Org Biomol Chem       Date:  2009-03-23       Impact factor: 3.876

9.  Fusion to a pull-down domain: a novel approach of producing Trigonopsis variabilisD-amino acid oxidase as insoluble enzyme aggregates.

Authors:  Jozef Nahalka; Bernd Nidetzky
Journal:  Biotechnol Bioeng       Date:  2007-06-15       Impact factor: 4.530

10.  Encapsulation of Trigonopsis variabilis D-amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme.

Authors:  Jozef Nahalka; Iskandar Dib; Bernd Nidetzky
Journal:  Biotechnol Bioeng       Date:  2008-02-01       Impact factor: 4.530
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