Literature DB >> 35089573

Characterization of the Conformational Properties of Soluble and Insoluble Proteins by Fourier Transform Infrared Spectroscopy.

Diletta Ami1, Antonino Natalello2.   

Abstract

The FTIR (micro-)spectroscopy method applied to the study of the structural properties of different soluble and insoluble proteins will be illustrated. In particular, we will discuss the procedure to analyze proteins in form of hydrated films and in solution by means of attenuated total reflection (ATR) measurements. Moreover, we will describe the procedure to characterize bacterial inclusion bodies (IBs) and amyloid deposits within human tissues by means of FTIR microspectroscopy.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Amyloid; Attenuated total reflection (ATR); Fourier transform infrared (FTIR) spectroscopy; Hydrated film; Inclusion bodies (IBs); Microspectroscopy; Protein aggregates; Protein structural properties; Secondary structures

Mesh：

Substances：

Year:  2022        PMID： 35089573     DOI： 10.1007/978-1-0716-1859-2_26

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  31 in total

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Authors:  S Seshadri; R Khurana; A L Fink
Journal:  Methods Enzymol       Date:  1999       Impact factor: 1.600

Review 2.  Structure and dynamics of membrane proteins as studied by infrared spectroscopy.

Authors:  J L Arrondo; F M Goñi
Journal:  Prog Biophys Mol Biol       Date:  1999       Impact factor: 3.667

Review 3.  The infrared absorption of amino acid side chains.

Authors:  A Barth
Journal:  Prog Biophys Mol Biol       Date:  2000       Impact factor: 3.667

Review 4.  Infrared spectroscopy of proteins and peptides in lipid bilayers.

Authors:  L K Tamm; S A Tatulian
Journal:  Q Rev Biophys       Date:  1997-11       Impact factor: 5.318

5.  Protein secondary structures in water from second-derivative amide I infrared spectra.

Authors:  A Dong; P Huang; W S Caughey
Journal:  Biochemistry       Date:  1990-04-03       Impact factor: 3.162

Review 6.  Insoluble protein assemblies characterized by fourier transform infrared spectroscopy.

Authors:  Antonino Natalello; Silvia M Doglia
Journal:  Methods Mol Biol       Date:  2015

Review 7.  What vibrations tell us about proteins.

Authors:  Andreas Barth; Christian Zscherp
Journal:  Q Rev Biophys       Date:  2002-11       Impact factor: 5.318

Review 8.  Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy.

Authors:  J L Arrondo; A Muga; J Castresana; F M Goñi
Journal:  Prog Biophys Mol Biol       Date:  1993       Impact factor: 3.667

Review 9.  Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. III. Secondary structures.

Authors:  E Goormaghtigh; V Cabiaux; J M Ruysschaert
Journal:  Subcell Biochem       Date:  1994

10.  Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy.

Authors:  Huayan Yang; Shouning Yang; Jilie Kong; Aichun Dong; Shaoning Yu
Journal:  Nat Protoc       Date:  2015-02-05       Impact factor: 13.491
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