Literature DB >> 35089565

Transient Gene Expression in Human Expi293 Cells.

Haroldo Cid da Silva Junior1.   

Abstract

Transient gene expression (TGE) is an important tool for generating recombinant proteins in a short period of time. The human cell line HEK293 is widely used for this purpose since it can grow in suspension to a high cell density in serum-free media. In addition, this cell line is amenable to several transfection methods and produces recombinant proteins in satisfactory quantities for functional and structural analysis. This chapter describes the methodology for TGE using the Expi293 system, which provides higher expression levels than other HEK293-based systems.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Expi293; Human cells; Recombinant protein; Transfection; Transient expression

Mesh：

Substances：

Year:  2022        PMID： 35089565     DOI： 10.1007/978-1-0716-1859-2_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Transient Gene Expression in Suspension HEK293-EBNA1 Cells.

Authors:  Denis L'Abbé; Louis Bisson; Christian Gervais; Eric Grazzini; Yves Durocher
Journal:  Methods Mol Biol       Date:  2018

Review 2.  Optimization of Protein Expression in Mammalian Cells.

Authors:  Molly Hunter; Ping Yuan; Divya Vavilala; Mark Fox
Journal:  Curr Protoc Protein Sci       Date:  2018-09-28

Review 3.  Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics.

Authors:  Sonia Gutiérrez-Granados; Laura Cervera; Amine A Kamen; Francesc Gòdia
Journal:  Crit Rev Biotechnol       Date:  2018-01-02       Impact factor: 8.429

4.  Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines.

Authors:  Javier Fuenmayor; Laura Cervera; Sonia Gutiérrez-Granados; Francesc Gòdia
Journal:  Appl Microbiol Biotechnol       Date:  2017-11-04       Impact factor: 4.813

Review 5.  HEK293 cell line: a vehicle for the expression of recombinant proteins.

Authors:  Philip Thomas; Trevor G Smart
Journal:  J Pharmacol Toxicol Methods       Date:  2005 May-Jun       Impact factor: 1.950

6.  Efficient and inexpensive transient expression of multispecific multivalent antibodies in Expi293 cells.

Authors:  Xiaotian T Fang; Dag Sehlin; Lars Lannfelt; Stina Syvänen; Greta Hultqvist
Journal:  Biol Proced Online       Date:  2017-09-15       Impact factor: 3.244

Review 7.  Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives.

Authors:  Jennifer Dumont; Don Euwart; Baisong Mei; Scott Estes; Rashmi Kshirsagar
Journal:  Crit Rev Biotechnol       Date:  2015-09-18       Impact factor: 8.429
  7 in total

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