Niyaz A Dar1, Mudasir A Mir1, Javid I Mir2, Sheikh Mansoor1, Wasia Showkat1, Tasmeen J Parihar1, Syed Anam Ul Haq1, Shabir H Wani3, Gul Zaffar4, Khalid Z Masoodi5. 1. Transcriptomics Laboratory (K-Lab), Division of Plant Biotechnology, SKUAST-Kashmir, Shalimar, Srinagar, Jammu and Kashmir, 190025, India. 2. Central Institute of Temperate Horticulture, Rangreth, Srinagar, Jammu and Kashmir, 191132, India. 3. Mountain Research Centre for Field Crops, SKUAST-Kashmir, Khudwani, Jammu and Kashmir, 192101, India. 4. Division of Plant Breeding & Genetics, SKUAST-Kashmir, Shalimar, Srinagar, Jammu and Kashmir, 190025, India. 5. Transcriptomics Laboratory (K-Lab), Division of Plant Biotechnology, SKUAST-Kashmir, Shalimar, Srinagar, Jammu and Kashmir, 190025, India. masoodikz@skuastkashmir.ac.in.
Abstract
AIM: Anthocyanin, an essential ingredient of functional foods, is present in a wide range of plants, including black carrots. The current investigation was carried out to analyse the effect of cold stress on the expression of major anthocyanins and anthocyanin biosynthetic pathway genes, MYB6 and LDOX-1. METHODS AND RESULTS: Five cultivated carrot genotypes belonging to the eastern group, having anthocyanin pigment, were used in the current study. The qRT-PCR analysis revealed that relative gene expression of transcription factor MYB-6 and LDOX1gene was highly expressed upon cold stress compared to non-stress samples. High-performance liquid chromatography-based quantification of Cyanidin 3-O-glucoside (Kuromanin chloride), Ferulic acid, 3,5-Dimethoxy-4-hydroxycinnamic acid (Sinapic acid), and Rutin revealed a significant increase in these major anthocyanins in response to cold stress when compared to control plants. CONCLUSION: We conclude that MYB6 and LDOX1 gene expression increases upon cold stress, which induces accumulation of major anthocyanins in purple black carrot and suggests a possible cross-link between cold stress and anthocyanin biosynthesis in purple black carrot.
AIM: Anthocyanin, an essential ingredient of functional foods, is present in a wide range of plants, including black carrots. The current investigation was carried out to analyse the effect of cold stress on the expression of major anthocyanins and anthocyanin biosynthetic pathway genes, MYB6 and LDOX-1. METHODS AND RESULTS: Five cultivated carrot genotypes belonging to the eastern group, having anthocyanin pigment, were used in the current study. The qRT-PCR analysis revealed that relative gene expression of transcription factor MYB-6 and LDOX1gene was highly expressed upon cold stress compared to non-stress samples. High-performance liquid chromatography-based quantification of Cyanidin 3-O-glucoside (Kuromanin chloride), Ferulic acid, 3,5-Dimethoxy-4-hydroxycinnamic acid (Sinapic acid), and Rutin revealed a significant increase in these major anthocyanins in response to cold stress when compared to control plants. CONCLUSION: We conclude that MYB6 and LDOX1 gene expression increases upon cold stress, which induces accumulation of major anthocyanins in purple black carrot and suggests a possible cross-link between cold stress and anthocyanin biosynthesis in purple black carrot.
Authors: Xianli Wu; Gary R Beecher; Joanne M Holden; David B Haytowitz; Susan E Gebhardt; Ronald L Prior Journal: J Agric Food Chem Date: 2006-05-31 Impact factor: 5.279