Literature DB >> 350869

N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli.

I K Dev, R J Harvey.   

Abstract

Glycinamide ribotide transformylase from Escherichia coli was obtained free of N5,N10-methenyltetrahydrofolate cyclohydrolase activity by DEAE-cellulose chromatography. In reaction mixtures containing this enzyme preparation in potassium maleate buffer, pH 7.2, no detectable interconversion of N5,N10-methenyltetrahydrofolate occurred. Upon addition of glycinamide ribotide, N-formylglycinamide ribotide was formed when N10-formyltetrahydrofolate was present; no formylation occurred in the presence of N5,N10-methenyltetrahydrofolate. A method for the synthesis and purification of glycinamide ribotide is presented.

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Year:  1978        PMID： 350869

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  7 in total

1.  Discovery of a potent, nonpolyglutamatable inhibitor of glycinamide ribonucleotide transformylase.

Authors:  Jessica K DeMartino; Inkyu Hwang; Lan Xu; Ian A Wilson; Dale L Boger
Journal:  J Med Chem       Date:  2006-05-18       Impact factor: 7.446

2.  Genetic and physiological characterization of a formate-dependent 5'-phosphoribosyl-1-glycinamide transformylase activity in Bacillus subtilis.

Authors:  H H Saxild; J H Jacobsen; P Nygaard
Journal:  Mol Gen Genet       Date:  1994-02

3.  Arabidopsis 10-formyl tetrahydrofolate deformylases are essential for photorespiration.

Authors:  Eva Collakova; Aymeric Goyer; Valeria Naponelli; Inga Krassovskaya; Jesse F Gregory; Andrew D Hanson; Yair Shachar-Hill
Journal:  Plant Cell       Date:  2008-07-15       Impact factor: 11.277

4.  Biological and structural evaluation of 10R- and 10S-methylthio-DDACTHF reveals a new role for sulfur in inhibition of glycinamide ribonucleotide transformylase.

Authors:  Stephen Connelly; Jessica K DeMartino; Dale L Boger; Ian A Wilson
Journal:  Biochemistry       Date:  2013-07-19       Impact factor: 3.162

5.  Asymmetric synthesis of inhibitors of glycinamide ribonucleotide transformylase.

Authors:  Jessica K DeMartino; Inkyu Hwang; Stephen Connelly; Ian A Wilson; Dale L Boger
Journal:  J Med Chem       Date:  2008-08-08       Impact factor: 7.446

6.  Heterologous expression and purification of active human phosphoribosylglycinamide formyltransferase as a single domain.

Authors:  C C Kan; M R Gehring; B R Nodes; C A Janson; R J Almassy; Z Hostomska
Journal:  J Protein Chem       Date:  1992-10

7.  Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.

Authors:  P Nygaard; J M Smith
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490
  7 in total

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