Literature DB >> 350837

Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase.

B K Duncan, P A Rockstroh, H R Warner.   

Abstract

A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA.

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Year:  1978        PMID： 350837      PMCID： PMC222353          DOI： 10.1128/jb.134.3.1039-1045.1978

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Mutants of Escherichia coli requiring methionine or vitamin B12.

Authors:  B D DAVIS; E S MINGIOLI
Journal:  J Bacteriol       Date:  1950-07       Impact factor: 3.490

Review 2.  Recalibrated linkage map of Escherichia coli K-12.

Authors:  B J Bachmann; K B Low; A L Taylor
Journal:  Bacteriol Rev       Date:  1976-03

3.  Mass screening for mutants with altered DNases by microassay techniques.

Authors:  B Weiss; C Milcarek
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

4.  Heat-induced deamination of cytosine residues in deoxyribonucleic acid.

Authors:  T Lindahl; B Nyberg
Journal:  Biochemistry       Date:  1974-07-30       Impact factor: 3.162

Review 5.  Pedigrees of some mutant strains of Escherichia coli K-12.

Authors:  B J Bachmann
Journal:  Bacteriol Rev       Date:  1972-12

6.  Mutants of Escherichia coli with altered deoxyribonucleases. I. Isolation and characterization of mutants for exonuclease 3.

Authors:  C Milcarek; B Weiss
Journal:  J Mol Biol       Date:  1972-07-21       Impact factor: 5.469

7.  New class of enzymes acting on damaged DNA.

Authors:  T Lindahl
Journal:  Nature       Date:  1976 Jan 1-8       Impact factor: 49.962

8.  Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis.

Authors:  J Duncan; L Hamilton; E C Friedberg
Journal:  J Virol       Date:  1976-08       Impact factor: 5.103

9.  N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2.

Authors:  E C Friedberg; A K Ganesan; K Minton
Journal:  J Virol       Date:  1975-08       Impact factor: 5.103

10.  An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues.

Authors:  T Lindahl
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
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  56 in total

1.  Context effects in the formation of deletions in Escherichia coli.

Authors:  T Kazic; D E Berg
Journal:  Genetics       Date:  1990-09       Impact factor: 4.562

2.  Genetic analysis of the LexA repressor: isolation and characterization of LexA(Def) mutant proteins.

Authors:  P Oertel-Buchheit; R M Lamerichs; M Schnarr; M Granger-Schnarr
Journal:  Mol Gen Genet       Date:  1990-08

3.  Uracil-DNA glycosylase causes 5-bromodeoxyuridine photosensitization in Escherichia coli K-12.

Authors:  Y Yamamoto; Y Fujiwara
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

4.  Escherichia coli strains with multiple DNA repair defects are hyperinduced for the SOS response.

Authors:  P L Foster
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

5.  Strand-biased cytosine deamination at the replication fork causes cytosine to thymine mutations in Escherichia coli.

Authors:  Ashok S Bhagwat; Weilong Hao; Jesse P Townes; Heewook Lee; Haixu Tang; Patricia L Foster
Journal:  Proc Natl Acad Sci U S A       Date:  2016-02-02       Impact factor: 11.205

6.  Uracil-DNA glycosylase affects mismatch repair efficiency in transformation and bisulfite-induced mutagenesis in Streptococcus pneumoniae.

Authors:  V Méjean; J C Devedjian; I Rives; G Alloing; J P Claverys
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

7.  Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli.

Authors:  B K Duncan; B Weiss
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

8.  Activation of Toxin-Antitoxin System Toxins Suppresses Lethality Caused by the Loss of σE in Escherichia coli.

Authors:  Yasushi Daimon; Shin-ichiro Narita; Yoshinori Akiyama
Journal:  J Bacteriol       Date:  2015-04-27       Impact factor: 3.490

9.  Isolation and partial characterization of a bacteriophage T5 mutant unable to induce thymidylate synthetase and its use in studying the effect of uracil incorporation into DNA on early gene expression.

Authors:  W J Swart; H R Warner
Journal:  J Virol       Date:  1985-04       Impact factor: 5.103

10.  Enzymatic degradation of uracil-containing deoxyribonucleic acid. V. Survival of Escherichia coli and coliphages treated with sodium bisulfite.

Authors:  R R Simmons; E C Friedberg
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490
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