Analysis of serum total bilirubin content based on dual-position joint spectrum of "M plus N" theory and the logarithmic method.

At present, in the chemical quantitative analysis of complex solutions based on spectroscopy, the accuracy of the content analysis of complex solution is difficult to meet the requirements due to the overlapping spectral lines, low signal-to-noise ratio, and scattering characteristics of various components of complex solution. In this paper, the logarithmic method is used to preprocess the spectral data in the spectral preprocessing stage, and the two-position and multi-mode joint spectral strategy of "M plus N" theory is applied to the quantitative analysis of serum components. The serum samples are illuminated by dual-position ultraviolet LED light source, and the two spectra of the vertical position and the coaxial facing position of the light source and the optical fiber are collected respectively. Then the partial least square (PLS) method was used to establish models to analyze the concentration of total bilirubin in serum by the spectrum of vertical position, the spectrum of coaxial facing position, and the spectrum of the combination of the former two. Among the experimental results, the model established by the combination of the two spectra collected by two positions has a good result. The correlation coefficient of all samples predicted by this model is 0.971223, and the root mean square error is 1.96645 μmol/L. This study shows that the method of the logarithmic, collecting spectra and analyzing the composition content of complex solutions by using the multi-location and multi-mode strategy of "M + N" theory can effectively improve the prediction accuracy of the model and has practical significance for the chemical quantitative analysis of complex solutions based on spectroscopy.