Literature DB >> 3507261

Gas chromatography-mass spectrometry analysis of tert.-butyldimethylsilyl derivatives of 2-acetylaminofluorene and metabolites in isolated rat hepatocytes.

M A Diez Ibañez1, M Chessebeuf-Padieu, P Nordmann, P Padieu.   

Abstract

A new technique for the conversion of 2-acetylaminofluorene and several ring-hydroxylated metabolites to mono- and di-tert.-butyldimethylsilyl derivatives was developed to permit their analysis by gas chromatography-mass spectrometry in order to quantify the metabolism of 2-acetylaminofluorene incubated in freshly isolated rat hepatocytes. This new gas chromatography-mass spectrometry method allowed the separation, identification and quantitation of seven known metabolites comprising five arylhydroxylated compounds, 2-aminofluorene and N-hydroxy-2-acetylaminofluorene.

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Year:  1987        PMID： 3507261     DOI： 10.1007/bf00117869

Source DB:  PubMed          Journal:  Cell Biol Toxicol        ISSN： 0742-2091            Impact factor:   6.691


  41 in total

1.  The N- and ring-hydroxylation of 2-acetylaminofluorene and the failure to detect N-acetylation of 2-aminofluorene in the dog.

Authors:  L A POIRIER; J A MILLER; E C MILLER
Journal:  Cancer Res       Date:  1963-06       Impact factor: 12.701

2.  Biological implications of the reaction possibilities of the proximate carcinogenic compound, N-hydroxy-2-fluorenylacetamide.

Authors:  G Lhoëst; C Razzouk; M Mercier
Journal:  Biomed Mass Spectrom       Date:  1976-02

3.  Activities of several phase I and phase II xenobiotic biotransformation enzymes in cultured hepatocytes from male and female rats.

Authors:  T Croci; G M Williams
Journal:  Biochem Pharmacol       Date:  1985-09-01       Impact factor: 5.858

4.  Enzymatic deacetylation of N-hydroxy-2-acetylaminofluorene by liver microsomes.

Authors:  C C Irving
Journal:  Cancer Res       Date:  1966-07       Impact factor: 12.701

5.  Gas chromatography mass spectrometry of tert-butyldimethylsilyl ethers of phthiocerols and mycocerosic alcohols from Mycobacterium tuberculosis.

Authors:  A I Mallet; D E Minnikin; G Dobson
Journal:  Biomed Mass Spectrom       Date:  1984-02

6.  Species differences in the metabolism of 2-acetylaminofluorene by hepatocytes in primary monolayer culture.

Authors:  J A Holme; B Trygg; E Søderlund
Journal:  Cancer Res       Date:  1986-04       Impact factor: 12.701

7.  N-Substituted aryl compounds in carcinogenesis and mutagenesis.

Authors:  E K Weisburger
Journal:  Natl Cancer Inst Monogr       Date:  1981-12

8.  N-acetoxy-N-acetylaminoarenes and nitrosoarenes. One-electron non-enzymatic and enzymatic oxidation products of various carcinogenic aromatic acethydroxamic acids.

Authors:  H Bartsch; J A Miller; E C Miller
Journal:  Biochim Biophys Acta       Date:  1972-06-26

9.  Effects of harman and norharman on the metabolism and genotoxicity of 2-acetylaminofluorene in cultured rat hepatocytes.

Authors:  J A Holme; E Søderlund; T Aune
Journal:  Cell Biol Toxicol       Date:  1985-06       Impact factor: 6.691

10.  [Biosynthesis of metabolites of 2-acetylaminofluorene by isolated hepatocytes of rats: study by gas chromatography-mass spectrometry].

Authors:  M Diez Ibanez; G Mignot; F Dhainaut; M Chessebeuf-Padieu; P Padieu
Journal:  C R Seances Soc Biol Fil       Date:  1986
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  2 in total

1.  Toxic effects of 7 beta-hydroxycholesterol on rat liver primary cultures, epithelial lines and co-cultures.

Authors:  P Nordmann; M Diez-Ibanez; M Chessebeuf-Padieu; B Luu; G Mack; M Mersel
Journal:  Cell Biol Toxicol       Date:  1989-11       Impact factor: 6.691

2.  Maintenance of liver function in long term culture of hepatocytes following in vitro or in vivo Ha-rasEJ transfection.

Authors:  M Fischbach; H W Cao; M Diez Ibanez; C Tsaconas; S Alouani; F Montandon; M el Baraka; P Padieu; M Dreano; M Chessebeuf-Padieu
Journal:  Cell Biol Toxicol       Date:  1991-10       Impact factor: 6.691
  2 in total

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