Daniela Fernanda Freitas1,2, David Fernando Colón3, Rangel Leal Silva3, Eloá Mangabeira Santos1, Victor Hugo Dantas Guimarães1, Guilherme Henrique Mendes Ribeiro4, Alfredo Maurício Batista de Paula1, André Luiz Sena Guimarães1, Sidnei Tavares Dos Reis4, Fernando Queiroz Cunha3, Maisa Mota Antunes5, Gustavo Batista Menezes5, Sérgio Henrique Sousa Santos6,7. 1. Laboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil. 2. Department of Pharmacy Course of College Saint Augustine, Montes Claros, Minas Gerais, Brazil. 3. Center for Research in Inflammatory Diseases (CRID), Department of Pharmacology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Brazil. 4. Institute of Agriculture Sciences, Departments of Food Engineering, Federal University of Minas Gerais (UFMG), Bairro Universitário, Montes Claros, Minas Gerais, Brazil. 5. Center for Gastrointestinal Biology, Departments of Morphology, Federal University of Minas Gerais, Minas Gerais, Brazil. 6. Laboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil. sergiosousas@hotmail.com. 7. Department of Pharmacy Course of College Saint Augustine, Montes Claros, Minas Gerais, Brazil. sergiosousas@hotmail.com.
Abstract
BACKGROUND: Neutrophil extracellular traps (NETs) are a recently discovered neutrophil defense mechanism which modulates several inflammatory conditions contributing to metabolic profile alterations. Therefore, the present study aimed to evaluate the production of NETs in obese patients and mice, verifying the possible mechanisms associated with the release of NETs by the adipose tissue. METHODS AND RESULTS: The present study investigated NETs production in human adipose tissue and also showing the neutrophils using intravital microscopy in mouse epididymal adipose tissue. Blood and white adipose tissues were obtained from eutrophic and obese individuals and from mice. Lipid, glycemic and leukocyte profiles were evaluated, as well as the levels of NETs and its markers. Bioinformatics and proteomics analyses were performed and the identified key proteins were measured. The main findings showed that the inflammatory markers interleukin-8 (IL-8), heat shock protein 90 (HSP90) and the E1 heat shock protein family (HSPE1) can be modulated by the NETs levels in obesity. Obesity has also been associated with increased cholesterol, glucose intolerance, ionic calcium and NETs. We also observed an increase in catalase and a decreased superoxide dismutase activity. Bioinformatics and proteomics analyses revealed that IL-8, HSP90 and HSPE1 were associated with obesity, inflammation and NETs release. CONCLUSIONS: In conclusion, the present study shows an increase in NETs production during obesity associated with important inflammatory markers in adipose.
BACKGROUND: Neutrophil extracellular traps (NETs) are a recently discovered neutrophil defense mechanism which modulates several inflammatory conditions contributing to metabolic profile alterations. Therefore, the present study aimed to evaluate the production of NETs in obese patients and mice, verifying the possible mechanisms associated with the release of NETs by the adipose tissue. METHODS AND RESULTS: The present study investigated NETs production in human adipose tissue and also showing the neutrophils using intravital microscopy in mouse epididymal adipose tissue. Blood and white adipose tissues were obtained from eutrophic and obese individuals and from mice. Lipid, glycemic and leukocyte profiles were evaluated, as well as the levels of NETs and its markers. Bioinformatics and proteomics analyses were performed and the identified key proteins were measured. The main findings showed that the inflammatory markers interleukin-8 (IL-8), heat shock protein 90 (HSP90) and the E1 heat shock protein family (HSPE1) can be modulated by the NETs levels in obesity. Obesity has also been associated with increased cholesterol, glucose intolerance, ionic calcium and NETs. We also observed an increase in catalase and a decreased superoxide dismutase activity. Bioinformatics and proteomics analyses revealed that IL-8, HSP90 and HSPE1 were associated with obesity, inflammation and NETs release. CONCLUSIONS: In conclusion, the present study shows an increase in NETs production during obesity associated with important inflammatory markers in adipose.
Authors: Stacy R Oliver; Jaime S Rosa; Ginger L Milne; Andria M Pontello; Holly L Borntrager; Shirin Heydari; Pietro R Galassetti Journal: Int J Pediatr Obes Date: 2010-10
Authors: Paul Poirier; Thomas D Giles; George A Bray; Yuling Hong; Judith S Stern; F Xavier Pi-Sunyer; Robert H Eckel Journal: Circulation Date: 2005-12-27 Impact factor: 29.690