Ghasem Ghasempour1,2, Asghar Mohammadi3, Fahimeh Zamani-Garmsiri4, Ali Akbar Soleimani1, Mohammad Najafi5,6,7. 1. Clinical Biochemistry Department, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. 2. Student Research Committee, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. 3. Clinical Biochemistry Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 4. Clinical Biochemistry Department, Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran. 5. Clinical Biochemistry Department, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. nbsmmsbn@iums.ac.ir. 6. Molecular and Cellular Research Center, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. nbsmmsbn@iums.ac.ir. 7. Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran. nbsmmsbn@iums.ac.ir.
Abstract
BACKGROUND: Mortality in patients with diabetes mellitus is estimated above 65% due to cardiovascular diseases. The aim of study was to investigate the effects of high-glucose conditions on TGF-β type II receptor (TGFBR2) expression levels, cell viability, and migration rate in vascular smooth muscle cells (VSMCs). METHODS: VSMCs were incubated in 30 mM and 50 mM of glucose for 24 h, 48 h, and 72 h periods. The gene and protein expression levels were investigated by Real-time qRT-PCR and western blotting techniques, respectively. The cell viability was evaluated by MTT assay. VSMC migration rate was also studied by wound healing assay. RESULTS: The TGFBR2 gene and protein expression levels were significantly upregulated in all the groups treated with glucose in 24 h, 48 h, and 72 h periods. The cell viability was not significantly affected in values of 30 mM and 50 mM of glucose. The increase of migration rate of VSMCs was not significant. CONCLUSION: The results suggested the increased expression levels of TGFBR2 in the response to high glucose conditions may modulate the cellular events through the signaling pathway network in VSMCs.
BACKGROUND: Mortality in patients with diabetes mellitus is estimated above 65% due to cardiovascular diseases. The aim of study was to investigate the effects of high-glucose conditions on TGF-β type II receptor (TGFBR2) expression levels, cell viability, and migration rate in vascular smooth muscle cells (VSMCs). METHODS: VSMCs were incubated in 30 mM and 50 mM of glucose for 24 h, 48 h, and 72 h periods. The gene and protein expression levels were investigated by Real-time qRT-PCR and western blotting techniques, respectively. The cell viability was evaluated by MTT assay. VSMC migration rate was also studied by wound healing assay. RESULTS: The TGFBR2 gene and protein expression levels were significantly upregulated in all the groups treated with glucose in 24 h, 48 h, and 72 h periods. The cell viability was not significantly affected in values of 30 mM and 50 mM of glucose. The increase of migration rate of VSMCs was not significant. CONCLUSION: The results suggested the increased expression levels of TGFBR2 in the response to high glucose conditions may modulate the cellular events through the signaling pathway network in VSMCs.
Authors: Paula S Azevedo; Bertha F Polegato; Marcos F Minicucci; Sergio A R Paiva; Leonardo A M Zornoff Journal: Arq Bras Cardiol Date: 2015-12-08 Impact factor: 2.000