Lara Stächele1,2, Daniel J Stekhoven3,4,5, Jan A Birzele6, Martin Risch7, Räto T Strebel6. 1. Departement of Urology, Kantonsspital Graubünden, Loëstrasse 170, 7000, Chur, Switzerland. lara@staechele.com. 2. Department of Neuro-Urology, Balgrist University Hospital, University of Zurich, Forchstrasse 340, 8008, Zürich, Switzerland. lara@staechele.com. 3. ETH Zurich, NEXUS Personalized Health Technologies, Wagistrasse 18, 8952, Schlieren, Switzerland. 4. SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland. 5. Clinical Bioinformatics Unit, NEXUS Personalized Health Technologies, HPL G 34.1, Otto-Stern-Weg 7, 8093, Zurich, Switzerland. 6. Departement of Urology, Kantonsspital Graubünden, Loëstrasse 170, 7000, Chur, Switzerland. 7. Central Laboratory, Kantonsspital Graubünden, Loëstrasse 170, 7000, Chur, Switzerland.
Abstract
PURPOSE: The primary objective of this preliminary study was to assess the changes in concentration of biomarkers, which indicate renal injury, after RIRS. MATERIALS AND METHODS: Within this prospective study, we included 21 patients with nephrolithiasis requiring treatment with RIRS. From each patient, blood and urine samples were taken at fixed intervals before and after RIRS. Kidney injury molecule-1 (KIM-1), monocyte chemoattractant protein 1 (MCP-1), neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), calbindin, albumin, clusterin, gluthation S-transferase-π (GST-π), beta-2-microglobulin (B2M), osteopontin, cystatin c, and trefoil-factor-3 (TFF3) were measured in urine. Creatinine, cystatin c and uric acid were analyzed in the blood samples. RESULTS: A significant increase of the biomarkers clusterin, GST-π, B2M, NGAL and cystatin c was observed after RIRS. However, the biomarkers gradually normalized during the first 14 postoperative days. The parameters surgery time, cumulative stone volume, and BMI did not significantly influence the biomarker concentrations. In the case of GST-π and NGAL a significant positive, yet minuscule effect of age was observed. CONCLUSIONS: With our study, we identified 5 out of 12 assessed renal injury biomarkers that showed a significant increase after RIRS. The increase was only temporary and all markers normalized within 14 days. Further studies are needed to determine the clinical value of these identified markers to assess the long-term impact of intrarenal pressure elevation during RIRS.
PURPOSE: The primary objective of this preliminary study was to assess the changes in concentration of biomarkers, which indicate renal injury, after RIRS. MATERIALS AND METHODS: Within this prospective study, we included 21 patients with nephrolithiasis requiring treatment with RIRS. From each patient, blood and urine samples were taken at fixed intervals before and after RIRS. Kidney injury molecule-1 (KIM-1), monocyte chemoattractant protein 1 (MCP-1), neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), calbindin, albumin, clusterin, gluthation S-transferase-π (GST-π), beta-2-microglobulin (B2M), osteopontin, cystatin c, and trefoil-factor-3 (TFF3) were measured in urine. Creatinine, cystatin c and uric acid were analyzed in the blood samples. RESULTS: A significant increase of the biomarkers clusterin, GST-π, B2M, NGAL and cystatin c was observed after RIRS. However, the biomarkers gradually normalized during the first 14 postoperative days. The parameters surgery time, cumulative stone volume, and BMI did not significantly influence the biomarker concentrations. In the case of GST-π and NGAL a significant positive, yet minuscule effect of age was observed. CONCLUSIONS: With our study, we identified 5 out of 12 assessed renal injury biomarkers that showed a significant increase after RIRS. The increase was only temporary and all markers normalized within 14 days. Further studies are needed to determine the clinical value of these identified markers to assess the long-term impact of intrarenal pressure elevation during RIRS.
Authors: Mario Sofer; James D Watterson; Timothy A Wollin; Linda Nott; Hassan Razvi; John D Denstedt Journal: J Urol Date: 2002-01 Impact factor: 7.450
Authors: Jean de la Rosette; John Denstedt; Petrisor Geavlete; Francis Keeley; Tadashi Matsuda; Margaret Pearle; Glenn Preminger; Olivier Traxer Journal: J Endourol Date: 2013-12-17 Impact factor: 2.942