| Literature DB >> 35055314 |
Ana C Quevedo1, Laura-Jayne A Ellis1, Iseult Lynch1, Eugenia Valsami-Jones1.
Abstract
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Year: 2022 PMID: 35055314 PMCID: PMC8781391 DOI: 10.3390/nano12020225
Source DB: PubMed Journal: Nanomaterials (Basel) ISSN: 2079-4991 Impact factor: 5.076
Figure 6Early endosome induction. The percentage of EE induction (EEI) in ZF4 cells treated with 2.5, 5, and 10 µg/mL of three different AgNP sizes (10, 30, and 100 nm) after (A) 2 and (B) 24 h. The intensity results were normalised to percentage (%) relative to the untreated cells (naive) (see Materials and Methods Section 2.6 for calculation). Data represent the mean of three individual replicates and above the bars the standard deviation (Mean ± SD). Data with asterisks (*) indicate a statistical difference (* p < 0.05) between the treatments compared to the untreated control (naive) at the specific timepoint.
Figure 7Autophagy induction in ZF4 cells exposed to AgNPs as determined by confocal microscopy. ZF4 Cells were treated with 2.5, 5, and 10 µg/mL AgNPs of three different sized AgNPs (10, 30, and 100 nm) for 24 h. Images of the cells with the nucleus (blue) and autophagosome staining (green) were taken at 60× with a NIKON A1R 808 series microscope (Nikon, Tokyo, Japan). A close-up of the labelled cells is marked with yellow lines. The intensity of the labelled autophagosomes in ZF4 cells was recorded by FIJI. (A) Results of three individual replicates are expressed as mean and standard deviation (Mean ± SD). A statistical comparison between all the treatments was performed. Data with asterisks (*) indicate a statistical difference (* p < 0.05) between the treatments compared to the untreated control (naive). The symbol above the bars (#) represents a statistically significant difference (# p < 0.05) between the marked treatments. (B) Cells treated with 10 µg/mL of different AgNPs sizes and the control. Images for the remaining AgNP concentrations and sizes can be found in the Supplementary Materials (Figure S4).