Phosphoethanolamine- and fructose 1,6-diphosphate-induced calcium uptake in bone formed in vitro.

The purpose of this investigation was to determine whether biologically available organic phosphates other than beta-glycerophosphate were capable of inducing mineralization of bone in vitro. The chick periosteal osteogenesis model was used to demonstrate that endogenously available organic phosphates, fructose 1,6-diphosphate (F1,6-D) and phosphoethanolamine (PEA) induce calcium accumulation in bone formed in vitro. Alkaline phosphatase activity was inhibited in a dose-dependent manner by PEA, and the sodium salt of F1,6-D. There was an inverse correlation between alkaline phosphatase activity and organic phosphate-mediated mineralization. The data demonstrate that certain biologically available organic phosphates can induce mineralization and modulate bone metabolism in vitro.