In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery.

The design of cancer immunotherapy drugs is essential for the continued investigation of novel drug regimens to improve responses and increase the survival of cancer patients. Methods to examine the interaction of effector immune cells with target cancer cells are limited by labor-intensive labeling that can be examined at specific time points. In this report, we examine an antigen-dependent model of effector cytotoxic (CD8+) T-cell-mediated cytotoxicity of target murine melanoma cells using a real-time cell impedance assay. The real-time monitoring allows measurement of viability and kinetics, allowing for a better understanding of effector/target cell interactions to support drug discovery.