Literature DB >> 3504128

A conjugate of trypsin and chymotrypsin.

Y S Rajput1, M N Gupta.   

Abstract

A heteroenzyme conjugate retaining activities of two component enzymes from trypsin and chymotrypsin was prepared using N-succinimidyl pyridyl dithiopropionate as crosslinking reagent. The conjugate bound to both trypsin and chymotrypsin affinity columns. Trypsin and chymotrypsin were linked in the ratio of 1:1 on mol basis. The conjugate, when treated with dimethyladipimidate, showed decreased autolysis of its trypsin component.

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Year:  1987        PMID： 3504128     DOI： 10.1007/BF02798367

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  9 in total

1.  Enzyme immunoassays in diagnostic medicine. Theory and practice.

Authors:  A Voller; D E Bidwell; A Bartlett
Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

2.  The preparation and properties of two new chromogenic substrates of trypsin.

Authors:  B F ERLANGER; N KOKOWSKY; W COHEN
Journal:  Arch Biochem Biophys       Date:  1961-11       Impact factor: 4.013

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

5.  Conjugation of glucose oxidase from Aspergillus niger and rabbit antibodies using N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide.

Authors:  S Yoshitake; Y Yamada; E Ishikawa; R Masseyeff
Journal:  Eur J Biochem       Date:  1979-11

6.  Lectins as carriers: preparation and purification of a concanavalin A-trypsin conjugate.

Authors:  W T Shier
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

7.  Preparation of the bifunctional enzyme ribonuclease-deoxyribonuclease by cross-linkage.

Authors:  D Wang
Journal:  Biochemistry       Date:  1979-10-02       Impact factor: 3.162

8.  Preparation of an enzymatically active cross-linked complex between brain cyclic nucleotide phosphodiesterase and 3-(2-pyridyldithio)propionyl-substituted calmodulin.

Authors:  R L Kincaid
Journal:  Biochemistry       Date:  1984-03-13       Impact factor: 3.162

9.  Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.

Authors:  J Carlsson; H Drevin; R Axén
Journal:  Biochem J       Date:  1978-09-01       Impact factor: 3.857
  9 in total

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