Literature DB >> 3504125

Molecular diagnostics using analytical immuno high performance liquid affinity chromatography.

P Caliceti1, G Fassina, I M Chaiken.   

Abstract

Analytical immuno high performance liquid affinity chromatography (analytical immuno HPLAC) was evaluated as a molecular diagnostic tool. Antibodies raised in rabbits against bovine neurophysin II were immobilized through Protein A crosslinking onto coated silica. Interaction of immobilized antibody with mobile antigen was characterized by zonal and frontal elutions of 14C-labeled bovine neurophysin II under isocratic, nondenaturing conditions. The chromatographic behavior shows that analytical immuno HPLAC with immobilized antibodies can be used to detect the number and functional nature of matrix-interacting antigens in mixtures, thus providing a quantitative chromatographic technology for "antigen mapping."

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3504125     DOI： 10.1007/BF02798361

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  12 in total

1.  Purification of bovine prothrombin by affinity chromatography.

Authors:  R Wallin; H Prydz
Journal:  FEBS Lett       Date:  1975-03-01       Impact factor: 4.124

2.  High performance liquid affinity chromatography (HPLAC) and its application to the separation of enzymes and antigens.

Authors:  S Ohlson; L Hansson; P O Larsson; K Mosbach
Journal:  FEBS Lett       Date:  1978-09-01       Impact factor: 4.124

3.  Analysis of ribonuclease-nucleotide interactions by quantitative affinity chromatography.

Authors:  I M Chaiken; H C Taylor
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

4.  Analytical high-performance affinity chromatography: evaluation by studies of neurophysin self-association and neurophysin-peptide hormone interaction using glass matrices.

Authors:  H E Swaisgood; I M Chaiken
Journal:  Biochemistry       Date:  1986-07-15       Impact factor: 3.162

5.  Quantitative high-performance affinity chromatography: evaluation of use for analyzing peptide and protein interactions.

Authors:  G Fassina; H E Swaisgood; I M Chaiken
Journal:  J Chromatogr       Date:  1986-04-11

6.  Preparative and analytical affinity chromatography of neurophysins on methionyl-tyrosyl-phenylalanyl-aminohexyl-agarose.

Authors:  I M Chaiken
Journal:  Anal Biochem       Date:  1979-09-01       Impact factor: 3.365

Review 7.  Analytical high-performance affinity chromatography.

Authors:  G Fassina; I M Chaiken
Journal:  Adv Chromatogr       Date:  1987

8.  High-performance affinity chromatography: a rapid technique for the isolation and quantitation of IgG from cerebral spinal fluid.

Authors:  T M Phillips; N S More; W D Queen; T V Holohan; N C Kramer; A M Thompson
Journal:  J Chromatogr       Date:  1984-12-28

9.  Protein A-coated glass beads. Universal support medium for high-performance immunoaffinity chromatography.

Authors:  T M Phillips; W D Queen; N S More; A M Thompson
Journal:  J Chromatogr       Date:  1985-06-26

10.  Molecular properties of the oxytocin/bovine neurophysin biosynthetic precursor. Studies using a semisynthetic precursor.

Authors:  T Kanmera; I M Chaiken
Journal:  J Biol Chem       Date:  1985-07-15       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.