| Literature DB >> 35037221 |
Abstract
Long noncoding RNAs (lncRNAs) are transcripts over 200 base pairs in length without discernible protein coding potential. Long intergenic noncoding RNAs (lincRNAs) constitute a subset of lncRNAs, which do not overlap protein coding genes. Here we describe a detailed pipeline for lincRNA discovery from publicly available non-stranded RNA-Seq datasets. The pipeline presented can be applied to any plant species for which RNA-Seq data and a reference genome sequence are available.Entities:
Keywords: Genome annotation; Long intergenic noncoding RNA; RNA-Seq; Transcriptome; lincRNA
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Year: 2022 PMID: 35037221 DOI: 10.1007/978-1-0716-2067-0_24
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745