Sympathetic neuronotrophic activity in the pulp of the cat canine tooth.

Extracts of these dental pulps from adult cats contained a non-dialysable agent or agents which could support neurone survival and neurite development for at least three days in neurone-enriched cultures of sympathetic ganglion cells from 11-day chick embryos. The neurone survival-promoting activity differed from nerve growth factor (NGF) in that: (1) anti-mouse NGF serum did not inhibit it; (2) nearly all ganglionic neurones survived in optimum concentrations of pulp extract, whereas only about 35 per cent were supported by NGF; and (3) cell bodies of NGF-supported neurones were markedly larger than in neurones supported by pulp extracts. The neuronotrophic activity in individual dental pulps was highly variable among different cats, but similar between mandibular canines from the same animal. Smaller pulps had higher concentrations of trophic activity than larger ones. Gingival tissue and the anterior belly of the disgastric muscle contained little neuronotrophic activity.